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Human Urogenital Myiasis Caused by Psychoda Species Larvae: Report of Five Cases and Morphological Studies

Research Abstract
Myiasis is the infestation of body tissues or organs by dipterous fly species and is often associated with poor hygiene. Although infestation by fly larvae is much more prevalent in animals, it occurs relatively frequent in humans in rural, tropical and subtropical regions of Africa and America. Urogenital myiasis is one of accidental myiasis that may be seen in humans. Urogenital myiasis is commonly associated with poor personal and environmental hygiene, low educational level and urogenital troubles. The current study presented five cases of urogenital myiasis. Patients were residing in Assuit and Qena Governorates (Upper Egypt). Some patients complained of intermittent passage of small, motile, greyish black wormiform objects in their urine and some were discovered accidentally. Larvae were collected and studied microscopically and detailed structures were described using scanning electron microscope (SEM). The larvae were morphologically identified as Psychoda spp. larvae. Special attention was given to cephalic region, vestiture, setae distribution and caudal extremity. It is worth mentioning that the sensillary necklace–like structure at the junction of the head with the first thoracic segment and the hollow appearance of setae were also clarified. It was concluded that despite the fact that urinary myiasis is very rare in humans; it should be considered in patients with urinary complaints.
Research Authors
Doaa A Yones1*, Hanaa Y Bakir1, Diaa A Hameed2
Research Department
Research Journal
The Journal of Advances in Parasitology
Research Pages
12-20
Research Publisher
Nexus® academic publishers
Research Rank
1
Research Vol
1-2
Research Website
http://dx.doi.org/10.14737/journal.jap/2014/1.2.12.20
Research Year
2014

Human Urogenital Myiasis Caused by Psychoda Species Larvae: Report of Five Cases and Morphological Studies

Research Abstract
Myiasis is the infestation of body tissues or organs by dipterous fly species and is often associated with poor hygiene. Although infestation by fly larvae is much more prevalent in animals, it occurs relatively frequent in humans in rural, tropical and subtropical regions of Africa and America. Urogenital myiasis is one of accidental myiasis that may be seen in humans. Urogenital myiasis is commonly associated with poor personal and environmental hygiene, low educational level and urogenital troubles. The current study presented five cases of urogenital myiasis. Patients were residing in Assuit and Qena Governorates (Upper Egypt). Some patients complained of intermittent passage of small, motile, greyish black wormiform objects in their urine and some were discovered accidentally. Larvae were collected and studied microscopically and detailed structures were described using scanning electron microscope (SEM). The larvae were morphologically identified as Psychoda spp. larvae. Special attention was given to cephalic region, vestiture, setae distribution and caudal extremity. It is worth mentioning that the sensillary necklace–like structure at the junction of the head with the first thoracic segment and the hollow appearance of setae were also clarified. It was concluded that despite the fact that urinary myiasis is very rare in humans; it should be considered in patients with urinary complaints.
Research Authors
Doaa A Yones1*, Hanaa Y Bakir1, Diaa A Hameed2
Research Department
Research Journal
The Journal of Advances in Parasitology
Research Pages
12-20
Research Publisher
Nexus® academic publishers
Research Rank
1
Research Vol
1-2
Research Website
http://dx.doi.org/10.14737/journal.jap/2014/1.2.12.20
Research Year
2014

Comparative Evaluation of Anthelmintic Activity of
Edible and Ornamental Pomegranate Ethanolic Extracts against
Schistosoma mansoni

Research Abstract
Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500
Research Authors
Doaa A. Yones,1 DaliaM. Badary,2 Hesham M. B. Sayed,3 Soad A. H. Bayoumi,3
Azza A. Khalifa,3 and AhmedM. El-Moghazy3
Research Journal
BioMed Research International
Research Pages
1-15
Research Publisher
Hindawi Publishing Corporation
Research Rank
1
Research Vol
2016
Research Website
Pomegranate- Schistosomicidial activity - In vitro - In vivo
Research Year
2016

Comparative Evaluation of Anthelmintic Activity of
Edible and Ornamental Pomegranate Ethanolic Extracts against
Schistosoma mansoni

Research Abstract
Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500
Research Authors
Doaa A. Yones,1 DaliaM. Badary,2 Hesham M. B. Sayed,3 Soad A. H. Bayoumi,3
Azza A. Khalifa,3 and AhmedM. El-Moghazy3
Research Journal
BioMed Research International
Research Pages
1-15
Research Publisher
Hindawi Publishing Corporation
Research Rank
1
Research Vol
2016
Research Website
Pomegranate- Schistosomicidial activity - In vitro - In vivo
Research Year
2016

Comparative Evaluation of Anthelmintic Activity of
Edible and Ornamental Pomegranate Ethanolic Extracts against
Schistosoma mansoni

Research Abstract
Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500
Research Authors
Doaa A. Yones,1 DaliaM. Badary,2 Hesham M. B. Sayed,3 Soad A. H. Bayoumi,3
Azza A. Khalifa,3 and AhmedM. El-Moghazy3
Research Journal
BioMed Research International
Research Pages
1-15
Research Publisher
Hindawi Publishing Corporation
Research Rank
1
Research Vol
2016
Research Website
Pomegranate- Schistosomicidial activity - In vitro - In vivo
Research Year
2016

Comparative Evaluation of Anthelmintic Activity of
Edible and Ornamental Pomegranate Ethanolic Extracts against
Schistosoma mansoni

Research Abstract
Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500
Research Authors
Doaa A. Yones,1 DaliaM. Badary,2 Hesham M. B. Sayed,3 Soad A. H. Bayoumi,3
Azza A. Khalifa,3 and AhmedM. El-Moghazy3
Research Journal
BioMed Research International
Research Pages
1-15
Research Publisher
Hindawi Publishing Corporation
Research Rank
1
Research Vol
2016
Research Website
Pomegranate- Schistosomicidial activity - In vitro - In vivo
Research Year
2016

Comparative Evaluation of Anthelmintic Activity of
Edible and Ornamental Pomegranate Ethanolic Extracts against
Schistosoma mansoni

Research Abstract
Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500
Research Authors
Doaa A. Yones,1 DaliaM. Badary,2 Hesham M. B. Sayed,3 Soad A. H. Bayoumi,3
Azza A. Khalifa,3 and AhmedM. El-Moghazy3
Research Department
Research Journal
BioMed Research International
Research Pages
1-15
Research Publisher
Hindawi Publishing Corporation
Research Rank
1
Research Vol
2016
Research Website
Pomegranate- Schistosomicidial activity - In vitro - In vivo
Research Year
2016

Comparative Evaluation of Anthelmintic Activity of
Edible and Ornamental Pomegranate Ethanolic Extracts against
Schistosoma mansoni

Research Abstract
Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500
Research Authors
Doaa A. Yones,1 DaliaM. Badary,2 Hesham M. B. Sayed,3 Soad A. H. Bayoumi,3
Azza A. Khalifa,3 and AhmedM. El-Moghazy3
Research Department
Research Journal
BioMed Research International
Research Pages
1-15
Research Publisher
Hindawi Publishing Corporation
Research Rank
1
Research Vol
2016
Research Website
Pomegranate- Schistosomicidial activity - In vitro - In vivo
Research Year
2016

Molecular and Conventional Detection of Zoonotic Giardia and Cryptosporidium in Children and Calves in Upper Egypt

Research Abstract
Giardia and Cryptosporidium are wide spread pathogens of human and many species of mammals. This study aimed to investigate the potential direct transmission of Giardia and Cryptosporidium between cattle calves and the surrounding living children and to improve the knowledge of zoonotic Giardiasis and cryptosporidiosis situation in Assiut Governorate, Egypt. Faecal samples from 70 diarrheic children (above 5 years) and 62 diarrheic calves (1-3 months) were collected from the same villages at Assiut Governorate, Egypt. Samples were subjected to conventional microscopic examination for Giardia and Cryptosporidium. Positive samples were subjected to molecular identification. By conventional microscopic examination, Giardia cysts were detected in 27 out of 70 (38.57%) child’s stool samples, while Cryptosporidium oocyst were detected in 12 samples (17.14%). Mixed infection of Giardia and Cryptosporidium were detected in all Cryptosporidium positive samples. In children, 81.48% of Giardia samples were G. intestinalis assemblage B meanwhile, all samples were negative by PCR for C. parvum. In calves 29.03% of faecal samples were positive for Giardia by microscopic examination, while only 12.90% of those samples were positive for Cryptosporidium oocysts. 21 out of 26 (80.76%) Giardia positive samples were positive to G. intestinalis assemblage B and 22 out of 26 Cryptosporidium positive samples (84.61%) were positive for C. parvum. High prevalence of G. intestinalis assemblage B infection detected among children was significantly associated with contact with calves explaining the existence of its zoonotic transmission and more studies are needed to investigate the zoonotic potential of Cryptosporidium in the study area.
Research Authors
Sylvia O. Ahmed1, Maha I. Hamed2,*, Doaa A. Yones3
Research Journal
American Journal of Infectious Diseases and Microbiology
Research Pages
91-94
Research Publisher
Science and Education Publishing
Research Rank
1
Research Vol
(4-4)
Research Website
DOI:10.12691/ajidm-4-4-4
Research Year
2016

Molecular and Conventional Detection of Zoonotic Giardia and Cryptosporidium in Children and Calves in Upper Egypt

Research Abstract
Giardia and Cryptosporidium are wide spread pathogens of human and many species of mammals. This study aimed to investigate the potential direct transmission of Giardia and Cryptosporidium between cattle calves and the surrounding living children and to improve the knowledge of zoonotic Giardiasis and cryptosporidiosis situation in Assiut Governorate, Egypt. Faecal samples from 70 diarrheic children (above 5 years) and 62 diarrheic calves (1-3 months) were collected from the same villages at Assiut Governorate, Egypt. Samples were subjected to conventional microscopic examination for Giardia and Cryptosporidium. Positive samples were subjected to molecular identification. By conventional microscopic examination, Giardia cysts were detected in 27 out of 70 (38.57%) child’s stool samples, while Cryptosporidium oocyst were detected in 12 samples (17.14%). Mixed infection of Giardia and Cryptosporidium were detected in all Cryptosporidium positive samples. In children, 81.48% of Giardia samples were G. intestinalis assemblage B meanwhile, all samples were negative by PCR for C. parvum. In calves 29.03% of faecal samples were positive for Giardia by microscopic examination, while only 12.90% of those samples were positive for Cryptosporidium oocysts. 21 out of 26 (80.76%) Giardia positive samples were positive to G. intestinalis assemblage B and 22 out of 26 Cryptosporidium positive samples (84.61%) were positive for C. parvum. High prevalence of G. intestinalis assemblage B infection detected among children was significantly associated with contact with calves explaining the existence of its zoonotic transmission and more studies are needed to investigate the zoonotic potential of Cryptosporidium in the study area.
Research Authors
Sylvia O. Ahmed1, Maha I. Hamed2,*, Doaa A. Yones3
Research Journal
American Journal of Infectious Diseases and Microbiology
Research Pages
91-94
Research Publisher
Science and Education Publishing
Research Rank
1
Research Vol
(4-4)
Research Website
DOI:10.12691/ajidm-4-4-4
Research Year
2016
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