In this paper, a design of multi-band microstrip antenna working as a transceiver in 0.5 ~ 3.8 GHz frequency range enhancing an imaging system of breast interior is presented. The slot loading technique is used to obtain multiple operating bands in the desired frequency range. The design procedure depends on parametric studies to choose best slots locations, widths and inter-separations for best performance. The aim is to obtain as many resonances as possible in the band of breast imaging. Having the most optimum design obtained, the antenna is fabricated and its characteristics are illustrated with a discussion of its potential use in a whole breast imaging system.