Aim: To assess the efficacy of Computer-guided open sinus lifting with simultaneous implant placement using a surgical guide versus the conventional technique.

Materials and Methods: Study was conducted as randomized control trail on 20 of patients were selected from the outpatient clinic of the Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Ain Shams University, and Assiut University. They are medically free patients seeking implant rehabilitation of posterior maxilla with pneumatization. These sinuses were randomly allocated into study group (A) 10 sinuses of computer-guided open sinus lift with simultaneous implant placement while control group (B) conventional technique. They have residual sub-antral bone height = 3 – 5 mm. Assessment of clinical parameters; intraoperative complications as Schneiderian membrane tear and bleeding, implant insertion torque, and surgical time. Assessment of radiographic parameters; preoperative, Postoperative, and gained vertical bone height measured by CBCT after 6 months.

Results: There was a statistically significant decrease in intraoperative complications and 50% of surgical time in computer guided group (A). While there was no statistically significant difference between the studied groups A and B as regards implant insertion torque and gained crestal bone height.

Conclusion: Computer-guided open sinus lifting technique is a double edge tool need to precise design and well-trained clinician to achieve accurate location of lateral windows and implants to avoid intraoperative complications with localized membrane elevation for a limited area in a short surgical time.

Keywords: Guided surgery, open sinus lift, Schneiderian perforation

Abstract

Aim: The objective of this study is to investigate the effect of fennel extract on tongue squamous cell carcinoma cell line and to assess the expression of the Bcl-2 gene in tongue squamous cell carcinoma cells treated with fennel extract.

Materials and Methods: The HNO-97 cell line of tongue squamous cell carcinoma was acquired from the Cell Culture Department NAWAH-Scientific in Cairo, Egypt. Fennel seed extraction was performed at NAWAHScientific in Cairo, Egypt. Cells' cytotoxicity was measured using the MTT assay. The expression of the Bcl-2 gene was assessed by western blotting technique. Cell death mode was identified through microscopic examination as well as image morphometric analysis. The outcomes underwent statistical testing through analysis of variance (ANOVA) test and Post Hoc test (Bonferroni).

Results: The MTT test indicated that the HNO-97 cells displayed sensitivity to fennel extract, showing an IC₅₀ of 2.80 mg/ml. Bcl-2 gene expression first decreased and then increased at post-IC₅₀ concentration, as determined by western blotting. The mean values of NAF decreased initially and then increased at post-IC₅₀ concentration, according to image morphometric analysis. The P-value was less than 0.05 so, the results were considered significant.

Conclusion: IC₅₀ concentration of fennel extract has the best cytotoxic effect on the HNO-97 cell line of tongue squamous cell carcinoma.