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Efficacy of n-alkyl dimethyl benzyl ammonium chloride and Bacillus subtilis for Control of Cercospora Leaf Spot Disease of Sugar Beet: In vitro and In vivo Studies.

Research Abstract

Cercospora leaf spot (CLS) is a serious sugar beet disease caused by the fungus Cercospora beticola that affects plants all over the world and causes significant yield losses in all cultivation areas. The main purpose of this study was to evaluate the antifungal activity of n-alkyl dimethyl benzyl ammonium chloride (n-ADBAC) and Bacillus subtilis against C. beticola, a pathogen causing Cercospora leaf spot of sugar beet, both in vitro and in vivo. Antifungal activity of n-ADBAC was tested in laboratory experiments against the pathogen’s growth at different concentrations, including 0.25, 0.50, 1.00, 1.50 and 2.00 g/L of water. At all concentrations examined, n-ADBAC was able to stop or limit the pathogen’s mycelial development. While the concentration of 2.00 g/L had a strong effect on pathogen growth inhibition (68.15%), it also had antifungal activity. Moreover, the results of antagonist assay of B. subtilis showed that it inhibits the growth of the causal pathogen, with an inhibition rate of up to 84.62%. Data also showed that application of n-ADBAC and B. subtilis as foliar treatments decreased the disease severity of Cercospora leaf spot under greenhouse and field conditions compared to untreated plants. The results revealed that n-ADBAC was the only treatment that consistently improved sugar beet agronomic characteristics, such as root yield and sugar content in roots compared to control plants. Data also showed that the tested treatments reduced the percentage of non-sugar substances, i.e. sodium, potassium and alpha-amino nitrogen in the roots.

 
Research Authors
Mansour M. El-Fawy, Abo-Elyousr KAM, Sherif A. Ahmed, Reda A. A. Korrat and Ayman S. Saeed
Research Date
Research Department
Research Journal
Gesunde Pflanzen
Research Pages
pages 2247–2256 -- https://doi.org/10.1007/s10343-023-00921-5
Research Publisher
Springer Berlin Heidelberg
Research Vol
75
Research Website
https://link.springer.com/article/10.1007/s10343-023-00921-5
Research Year
2023

Exploring the mechanisms of endophytic bacteria for suppressing early blight disease in tomato (Solanum lycopersicum L.)

Research Authors
Nashwa M. A. Sallam, Heba-Alla S Abdelatah, Hadeel M M Khalil Bagy, Ameer Elfarash, Kamal A.M. Abo-Elyousr, and Ahmed Sallam.
Research Date
Research Department
Research Pages
https://doi.org/ 10.3389/fmicb.2023.1184343
Research Publisher
Front. Microbiol. - Food Microbiology Front. 14
Research Year
2023

Effectiveness of Bacillus cereus in Controlling Potato Bacterial Wilt Caused by Ralstonia solanacearum: Greenhouse and Field Studies with Insights into Resistance-Related Enzymes in Potatoes

Research Authors
Mohamed A. A. Seleim, Mohamed F.F. Bereika, Omer H.M. Ibrahim, Ahmed I. Alqubaie and Abo-Elyousr KAM
Research Date
Research Department
Research Publisher
Plant Diseases and Protection
Research Year
2023

Synergistic effect of Bacillus subtilis and Benzothiadiazole (Bion®) on the suppression of Fusarium oxysporum and the enhancement of disease resistance in Capsicum annuum.

Research Abstract
  1. (2023).
    Plant Pathology Journal
Research Authors
Abo-Elyousr KAM, Nashwa M.A. Sallam , Magdy A. A. Mousa, Muhammad Imran and Ismail R. Abdel-Rahim
Research Date
Research Department
Research Publisher
Plant Pathology Journal
Research Year
2023

The mitigating effect of Rhodotorula mucilaginosa on Fusarium wilt-induced stress in tomato plants under different irrigation levels

Research Authors
Mohamed Hassan Abd El-Wahed, Najeeb M. Almasoudi, Abo-Elyousr KAM and Hadeel M. Khalil Bagy
Research Date
Research Department
Research Pages
https://doi.org10.1007/s42161-023-01528-5
Research Publisher
Plant Pathology Journal
Research Year
2023

Effects of saline water on soil properties and red radish growth in saline soil as a function of co-applying wood chips biochar with chemical fertilizers

Research Abstract

Background Currently, using unconventional water sources in agriculture has become necessary to face overpopulation worldwide. Therefore, a pot experiment was carried out to evaluate the effects of irrigation with saline water in the presence of co-applied wood chips biochar (WCB) with chemical fertilizers on physicochemical properties and nutrient availability as well as growth parameters, and yield of red radish (Raphanus sativus L.) grown in the saline sandy soil.

 

Methods The WCB was added to the saline sandy soil at levels of 0 (control), 2.5, and 5% w/w. Then, this soil was cultivated by red radish plants and irrigated with saline water (5 dS m- 1). This experiment was performed in a randomized complete block design with three replicates.

 

Results Compared with the control treatment, WCB treatments increased significantly soil water holding capacity by 34.8% and 73.2% for levels of 2.5 and 5%, respectively. Soil pH decreased significantly in all WCB treatments. The relative increase in the total available nitrogen over the control was 30.1 and 103.5% for 2.5 and 5% wood chips biochar, respectively. Compared to the control, applying WCB at 2.5% led to an increase in the fresh root weight of red radish plants by 142.7%, while 5% caused a decrease in the fresh root weight of red radish plants by 29.4%. Conclusion Recently, WCB represents an interesting approach to the rehabilitation of saline soils and the management of using saline water sources. It is recommended that combined application of WCB at a level of 2.5% with chemical fertilizers in order to improve red radish growth and nutrient retention in the saline sandy soil which preserves the ecosystem as well as increases productivity leading to the reduction of costs.

 

Research Authors
Abu El-Eyuoon Abu Zied Amin
Research Date
Research Department
Research Journal
BMC Plant Biology
Research Publisher
Springer Nature
Research Website
https://doi.org/10.1186/s12870-023-04397-3
Research Year
2023

Characterization of aroma profiles of chinese four most famous traditional red-cooked chickens using GC–MS, GC-IMS, and E-nose

Research Abstract

The aroma profile of the four most popular types of red-cooked chickens in China was analyzed using a combination of gas chromatography-mass spectrometry (GC–MS), gas chromatography-ion mobility spectrometry (GC-IMS), and electronic nose (E-nose). Principal component analysis (PCA) demonstrated that the E-nose could successfully distinguish between the four types of red-cooked chickens. Additionally, a fingerprint was created using GC-IMS to examine the variations in volatile organic compounds (VOCs) distribution in the four chicken types. A total number of 84 and 62 VOCs were identified in the four types of red-cooked chickens using GC–MS and GC-IMS, respectively. Odor activity value (OAV) showed that 1-octen-3-ol, heptanal, hexanal, nonanal, octanal, eugenol, dimethyl trisulfide, anethole, anisaldehyde, estragole, and eucalyptol were the key volatile components in all samples. Furthermore, partial least squares-discriminant analysis (PLS-DA) demonstrated that (E, E)-2,4-decadienal, dimethyl trisulfide, octanal, eugenol, hexanal, (E)-2-nonenal, 1-octen-3-ol, butanal, ethyl acetate, ethyl acetate (D), nonanal, and heptanal could be used as markers to distinguish aroma of the four types of red-cooked chickens. Also, it is worth noting that levels of VOCs varied between chicken breast muscle and skin. The obtained results offer theoretical and technological support for flavor identification and control in red-cooked chickens to enhance their quality and encourage consumer consumption, which will be advantageous for the red-cooked chicken production chain. 

Research Authors
Xiangxiang Sun, Yumei Yu, Ahmed SM Saleh, Xinyu Yang, Jiale Ma, Ziwu Gao, Dequan Zhang, Wenhao Li, Zhenyu Wang
Research Date
Research Journal
Food Research International
Research Member
Research Publisher
Elsevier
Research Vol
173
Research Website
https://www.sciencedirect.com/science/article/abs/pii/S0963996923008803
Research Year
2023

Exploring the interaction between myofibrillar proteins and pyrazine compounds: Based on molecular docking, molecular dynamics simulation, and multi-spectroscopy techniques

Research Abstract

Flavor is one of the most important factors that affect consumers' preference for processed meat products. This study aimed to investigate effects of heating on interaction between myofibrillar proteins (MPs) and pyrazine compounds and understand the underlying mechanisms. A combination of multispectral, molecular docking, and molecular dynamics technologies was used to achieve study's aim. Results demonstrated that MPs underwent structural reconstruction and expansion during heating, which significantly altered surface hydrophobicity and SH content. MPs' zeta potential reduced from −7.29 to −10.47 when a short heating time. Additionally, a positive correlation was found between β-sheet content and ability of MPs to adsorb pyrazine compounds. Molecular docking analysis revealed 13 binding sites for pyrazines and MPs. Furthermore, amino acid residues and pyrazine compounds were found to interact by four different forms of forces, primarily van der Waals forces, carbon‑hydrogen bonds, alkyl groups, and π-alkyl groups. Obtained results demonstrated that adequate or optimized heat treatment could expose more binding sites, hence enhancing the binding of MPs to pyrazine compounds. This study may be used to better understand how structural changes in MPs during processing affect MPs' capacity to bind flavor substances, which can help improve flavor of processed meats to encourage their consumption.

Research Authors
Yumei Yu, Ahmed SM Saleh, Xiangxiang Sun, Zhenyu Wang, Yang Lu, Dequan Zhang, Chunjiang Zhang
Research Date
Research Journal
International Journal of Biological Macromolecules
Research Member
Research Publisher
Elsevier
Research Vol
253
Research Website
https://www.sciencedirect.com/science/article/abs/pii/S0141813023037418
Research Year
2023

Plant phenolic extracts for the quality protection of frying oil during deep frying: Sources, effects, and mechanisms

Research Abstract

Protection of frying oil from deterioration by adding plant phenolic extracts to guarantee the quality of fried foods becomes the primary approach to promote the sustainable development of deep frying. Therefore, sources, antioxidant effects, and mechanisms of plant phenolic extracts recently applied in the quality protection of frying oil as well as challenges for the actual use of these extracts are comprehensively reviewed in this study. Spices, herbs, berries, tea leaves, and fruit and vegetable wastes are common sources for preparing phenolic extracts showing comparative antioxidant capacity referring to the synthetic antioxidants. The general effect of using these natural antioxidants is the improvement of thermal stability to extend the shelf life of frying oil and thus the modification of edible quality of fried foods. Specifically, the increases in common quality attributes and amount of hazardous products and the oxidative reduction of unsaturated triacylglycerols without negatively influencing the sensory quality are inhibited when suitable plant extracts are applied. The incorporation of plant phenolic extracts other than synthetic counterparts in frying oil has been demonstrated as a potential method to improve the frying performance of oils. However, challenges for the scale application of plant phenolic extracts, such as the purity, thermal stability, and antioxidant timing, are still needed to be further investigated.

Research Authors
Fa Wang, Yixi Sun, Shanshan Li, Jing Yan, Wen Qin, Ahmed SM Saleh, Qing Zhang
Research Date
Research Journal
Grain & Oil Science and Technology
Research Member
Research Publisher
Elsevier
Research Website
https://www.sciencedirect.com/science/article/pii/S2590259823000195
Research Year
2023

Structural changes induced by ultrasound improve the ability of the myofibrillar protein to bind flavor compounds from spices

Research Abstract

Effects of ultrasound (UT) treatments on the structural, physicochemical, and functional properties of myofibrillar proteins (MPs), as well as their ability to bind to flavor compounds from spices, were investigated. The results demonstrated that UT treatment enhanced surface hydrophobicity, SH content, and absolute ζ-potential value of the MPs. Atomic force microscopy analysis displayed formation of MPs aggregates with small particle size in the UT-treated MPs samples. Meanwhile, UT treatment could improve the emulsifying properties and physical stability of MPs’ emulsion. Additionally, the MPs gel network structure and stability significantly improved following UT treatment. Changes in the structural, physicochemical, and functional properties enhanced the ability of MPs to bind to flavor substances from spices depending on the duration of UT treatment. Furthermore, correlation analysis showed that the ability of myristicin, anethole, and estragole to bind to MPs was highly correlated with surface hydrophobicity, ζ-potential value, and α-helix content of MPs. The results of this study may help in understanding the relationship between the changes in MPs properties during the processing of meat products and their ability to bind to flavors from spices, thereby improving flavors retention and taste of processed meat products. 

Research Authors
Xiangxiang Sun, Yumei Yu, Ahmed SM Saleh, Xinyu Yang, Jiale Ma, Ziwu Gao, Wenhao Li, Zhenyu Wang, Dequan Zhang
Research Date
Research Journal
Ultrasonics Sonochemistry
Research Member
Research Pages
106510
Research Publisher
Elsevier
Research Vol
98
Research Website
https://www.sciencedirect.com/science/article/pii/S1350417723002225
Research Year
2023
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