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Echocardiographic characterization of cardiac chambers and vasculatures in buffaloes (Bubalus bubalis) during diastolic and systolic phases

Research Abstract

Abstract
Background:
Ultrasonography had diagnostic importance in the evaluation of different diseases in buffaloes, including cardiovascular diseases.
Aim:
The current work describes the normal echocardiographic findings in healthy buffaloes, along with establishing reference values for echocardiographic dimensions for both sides of the heart i.e. left and right ones.
Methods:
About 30 healthy adult buffaloes that belonged to private farms in Assiut, Egypt, were included in this study. Each animal underwent a complete clinical evaluation as well as haematological analyses, lipid profile indices, liver functions, cardio-thoracic radiography and echocardiography to confirm no diseased conditions were detected. The study was conducted on healthy buffaloes (n = 30) in Assiut Governorate, Egypt.
Results:
The obtained results reported healthy buffaloes with normal clinical findings as well as indices of blood pictures and serum biochemicals that were within the reference intervals. Radiography revealed free reticulum and a well-defined diaphragm. The heart was seen as a typical radio-opaque organ. Ultrasonographically, using gray scale B-mode and M-mode, the heart was commonly imaged from the left 4th intercostal space. Different echocardiographic views were described, including the 4 chamber view, i.e., right atrium (RA), right ventricle (RV), tricuspid valve (TCV), left atrium (LA), left ventricle (LV), mitral valve (MV), and interventricular septum (IVS), and the right ventricular outflow tract (RVOT), i.e., Right atrium (RA), tricuspid valve (TCV), right ventricle (RV), pulmonary artery (PA), and pulmonary valve (PV). Cross sections in each of the apex and base of the heart were described. Echocardiographic dimensions during cardiac diastole and systole included diameters and wall thickness of each of atria and ventricles were demonstrated. Interventricular septal thickness wall thickness as well as diameters of aorta and pulmonary artery were stated.
Conclusion:
The work tried to put reference values on the normal echocardiographic dimensions using two-dimensional B mode gray scale ultrasonography in healthy adult buffaloes. These echocardiographic reference dimensions with normal echocardiographic imaging will be very helpful in enhancing the diagnostic efficacy of ultrasounds for recognizing the abnormal findings related to cardiac disorders.

Research Authors
Arafat Khalphallah, Enas Elmeligy, Khaled A. Khesruf, Haitham H. Mohammed H. Mohammed, Abdulrahman Abdulkarim, Ashraf M. Abu-Seida, Al-lethie A. Al-lethie
Research Date
Research Department
Research Journal
Open Veterinary Journal
Research Pages
1239-1250
Research Publisher
https://www.openveterinaryjournal.com/
Research Rank
q2
Research Vol
13 (10)
Research Website
https://www.openveterinaryjournal.com/?mno=154347
Research Year
2023

Congratulations - The Faculty volleyball team won third place at the Assiut University level

تحت رعاية السيدة الأستاذ الدكتور / مديحه درويش عميد الكلية والمشرف العام علي الانشطه الطلابيه بالجامعة
والسيد الاستاذ الدكتور / مؤمن عبد العظيم وكيل الكلية لشئون التعليم والطلاب 
فوز فريق الكلية للكرة الطائرة بالمركز الثالث علي مستوي جامعة أسيوط بعد فوزه علي كلية الصيدليه

مشرف الفرق ا. محمد حسن

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An integrated calibration strategy for the development and validation of an LC-MS/MS method for accurate quantification of egg allergens (Gal d 1–6) in foods

Research Abstract

Accurate determination of egg allergens in food is vital for allergen management and labeling. However, quantifying egg allergens with mass spectrometry poses challenges and lacks validation methods. Here, we developed and validated an LC-MS/MS method for quantifying egg allergens (Gal d 1–6) in foods. Sample extraction, enzymatic digestion, purification, proteins/peptides selection, and calibration curves were optimized. VMVLC[+57]NR (Gal d 1) and GTDVQAWIR (Gal d 5) exhibited outstanding sensitivity and stability, serving as quantitation markers for egg white and yolk. Using a matrix-matched calibration curve with allergen ingredients as calibrants and labeled peptides as standards, we achieved highly accurate quantitation. Validation involved spiking egg protein into egg-free foods, showing excellent sensitivity (LOQ: 1–5 mg/kg), accuracy (62.4 %–88.5 %), and reproducibility (intra-/inter-day precision: 3.5 %–14.2 %/8.2 %–14.6 %). Additionally, we successfully applied this method to commercial food analysis. These findings demonstrate optimal allergen selection, peptides, and calibration strategy are crucial parameters for food allergen quantification via MS-based methods.

Research Authors
Shupeng Yang , Jingjing Chen, Mohamed F. Abdallah, Haopeng Lin, Peijie Yang, Jianxun Li, Rong Zhang, Qianqian Li, Peng Lu, Suyan Liu, Yi Li
Research Date
Research Department
Research File
Research Journal
Food Chemistry
Research Member
Research Pages
137922
Research Publisher
Elsevier
Research Rank
Q1
Research Website
https://www.sciencedirect.com/science/article/pii/S0308814623025402
Research Year
2023

Immunopathogenesis of the Canadian Delmarva (DMV/1639) infectious bronchitis virus (IBV): Impact on the reproductive tract in layers

Research Authors
Mohamed SH Hassan, Shahnas M Najimudeen, Ahmed Ali, Danah Altakrouni, Dayna Goldsmith, Carla S Coffin, Susan C Cork, Frank van der Meer, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Microbial Pathogenesis
Research Pages
105513
Research Publisher
Academic Press
Research Vol
166
Research Website
https://sciencedirect.com/science/article/pii/S0882401022001267
Research Year
2022

Replication of infectious bronchitis virus (IBV) Delmarva (DMV)/1639 variant in primary and secondary lymphoid organs leads to immunosuppression in chickens

Research Authors
Shahnas M Najimudeen, Reham M Abd-Elsalam, Hiruni A Ranaweera, Ishara M Isham, Mohamed SH Hassan, Muhammad Farooq, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Virology
Research Pages
109852
Research Publisher
Academic Press
Research Vol
587
Research Website
https://www.sciencedirect.com/science/article/pii/S0042682223001654
Research Year
2023

Impact of Maternal Antibodies on Infectious Bronchitis Virus (IBV) Infection in Primary and Secondary Lymphoid Organs of Chickens

Research Authors
Ishara M Isham, Mohamed SH Hassan, Reham M Abd-Elsalam, Hiruni A Ranaweera, Motamed E Mahmoud, Shahnas M Najimudeen, Awais Ghaffar, Susan C Cork, Ashish Gupta, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Vaccines
Research Pages
1216
Research Publisher
MDPI
Research Vol
11 (7)
Research Website
https://www.mdpi.com/2076-393X/11/7/1216
Research Year
2023

The impact of the experimental route of challenge on the host responses and pathogenesis of the Canadian Delmarva (DMV/1639) infectious bronchitis virus infection in laying chickens

Research Authors
Mohamed SH Hassan, Reham M Abd-Elsalam, Natalya Ratcliff, Heshanthi Herath-Mudiyanselage, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Veterinary Immunology and Immunopathology
Research Pages
110623
Research Publisher
Elsevier
Research Vol
261
Research Website
https://www.sciencedirect.com/science/article/abs/pii/S0165242723000776
Research Year
2023
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