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Differential diagnosis of bovine intestinal diseases and their sequelae regarding ultrasonography and other diagnostic tools

Research Abstract

Background and Aim: Intestinal disorders represented a large proportion of abdominal emergencies in bovine practice, and their definite diagnosis was a big challenge. The study described different intestinal disorders in cattle either in the small intestine (SI) or large intestine with their sequelae and peritonitis between SI loops.
Materials and Methods: This study involved healthy (n=20) and diseased (n=40) cattle with intestinal disorders. All animals were undergoing clinical examination, laboratory analyses, and ultrasonographic examination.
Results: Diseased cattle had monocytic leukocytosis as well as hypoproteinemia and hypoalbuminemia. The SI ileus of either proximal (n=12) or distal (n=15), intussusception (n=3), cecal, and/or colonal dilatation (n=10) were diagnosed by ultrasound and other diagnostic tools. Duodenum intussusception was imaged in cross-section as bull’s eyes lesions. Animals with SI obstructions showed complete cessation (ileus with complete obstruction) or partial reduction of the peristaltic SI movement (ileus with partial obstruction), dilated duodenum (6.5-9.9  cm), and dilated jejunum and/or ileum (4.4-6.8  cm). Ultrasonography diagnosed SI ileus, due to either intestinal obstruction or peritonitis, and detected the ileus site, either proximal or distal. Cecal/colonal dilatation was detected using ultrasonography in which SI was not imaged and the peristaltic movements were completely reduced. The recorded intestinal disorders were associated with other disorders (e.g.,   liver cirrhosis or peritonitis).
Conclusion: Ultrasonography played an important role in the differential diagnosis of intestinal disorders in cattle. Peritonitis between SI loops and cecal and/or colonal dilatation was also diagnosed.

Research Authors
Arafat Khalphallah, Hanan K. Elsayed, Enas Elmeligy, Sara A. Bayomi, Mohamed A. Hamed, Doaa Salman, Ashraf M. Abu-Seida and Sabry A. Mousa
Research Date
Research Department
Research Journal
Veterinary World, EISSN: 2231-0916
Research Pages
1537-1547
Research Publisher
Veterinary World, EISSN: 2231-0916
Research Rank
q2: https://www.scimagojr.com/journalsearch.php?q=21100201717&tip=sid&clean=0
Research Vol
https://www.veterinaryworld.org/
Research Website
https://www.veterinaryworld.org/
Research Year
2021

ENTERIC ROTA AND CORONA VIRUSES INFECTION IN NEONATAL CALVES

Research Abstract

 

Calf diarrhea is a common syndrome causing colossal economic losses.Rotavirus and Coronavirus are major pathogens of calf diarrhea. During the period of investigation (13 consecutive months), a total number of 140 neonatal calves were carefully examined and thereafter subjected to serological and molecular diagnosis for the diagnosis of Rota and Corona viruses' infection. The serological and molecular diagnosis by using LAT and RT-PCR indicated that positive samples of Rotavirus infection were 21.43% (30/140) and 8% (4/50), respectively. ELISA and RT-PCR were used in diagnosing of BCoV infection and found that positive samples were 2.13% (2/94) and 4% (2/50), respectively. The Prevalence of Rotavirus and Coronavirus infection was 21.43 % (30/140) and 2.13 % (2/94) of the examined calves, respectively. It was found that there was a strong negative correlation between age of examined calves and Rotavirus infection. Coronavirus infection was found in calves at >4-7 weeks. There were no significance difference in Rota and Corona viruses' infection and sex, breed and species of examined calves. The most positive cases of Rotavirus and Coronavirus infection were reported in cold months. The lower infection rate of Rotavirus was recorded in hot months.It is concluded that Rota and Corona viruses play an outstanding role in causing enteritis in neonatal calves in different localities of Assiut governorate.

Research Authors
AHMED MA ZAITOUN, OSAMA ABDEL-HAKIM, ZAINAB MA YOUSSEF
Research Date
Research Department

Antioxidant and cytoprotective effects of Nigella sativa L. seeds on the testis of monosodium glutamate challenged rats

Research Abstract

Monosodium glutamate (MSG) is one of the most widely spread food additives that might cause male
infertility. However,
Nigella sativa L. seeds (NSS) could provide a solution. This study was designed
to investigate the potential effects of NSS on rats ingesting MSG. To achieve this aim, adult male
albino rats were randomly equally assigned into three groups for 21 days: control group received no
treatment, MSG group received MSG as 30 g/kg feed, and MSG + NSS group received MSG as 30 g/kg
and NSS as 30 g/kg feed. Testis histomorphometry showed marked deterioration by MSG as atrophic
seminiferous tubules with degeneration of their lining cells, damaged Leydig cells and decreased
germ cells number. Periodic Acid Schiff stain indicated irregular interrupted basement membranes.
Glutathione reductase, superoxide dismutase 2 (SOD2), and caspase‑3 immuno‑expressions increased
in testicular cells. Testosterone levels were significantly decreased in MSG challenged rats along with
significant increase in luteinizing hormone levels, whereas NSS normalized this hormonal profile.
MSG exposure also caused significantly increased lipid peroxides (LPO), glutathione‑
S‑transferase,
and total antioxidant capacity (TAC) whereas nitric oxide and SOD2 were significantly decreased.
NSS succeeded in rebalance LPO and TAC and ameliorated the histoarchitectural disturbances. NSS
mitigated MSG‑induced testicular impairment by its antioxidant and cytoprotective activities.

 

Research Authors
Mahmoud Abd‑Elkareem, Mokhless A. M. Abd El‑Rahman, Nasser S. Abou Khalil & Ayman S. Amer
Research Date
Research File
Research Journal
Scientific reports
Research Member
Research Pages
16
Research Publisher
Nature publishing group
Research Rank
Q1
Research Vol
11
Research Website
https://www.nature.com/articles/s41598-021-92977-4
Research Year
2021
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