Salinomycin (SAL) is widely used as an anticoccidial agent in poultry production; however, its residue accumulation in edible tissues raises significant food safety concerns. Therefore, accurate and sensitive determination of SAL levels in chicken tissues is essential for consumer protection and regulatory compliance. In this study, a novel dual-emission fluorometric method was developed for the detection of SAL, utilizing a ratiometric sensing platform based on rhodamine 6G (Rh6G) and red-emitting carbon dots (R/CDs). Under a single excitation wavelength of 530 nm, Rh6G exhibited a fluorescence emission at 550 nm, while R/CDs emitted at 610 nm. SAL selectively enhanced the Rh6G signal without affecting the R/CDs emission, enabling a robust and reliable ratiometric response for quantification. The developed system demonstrated a wide linear response range, both in standard solutions (1.0–60.0 ng/mL) and in spiked tissue matrices (10.0–600.0 μg/kg), confirming its suitability for real-sample analysis. Extraction efficiencies were high across tested tissues, ranging from 94.51 to 97.50 % in liver, 95.54 to 98.90 % in kidney, and 98.83 to 100.20 % in skin/fat samples. Moreover, the method was successfully applied to monitor SAL residue depletion in edible tissues over time, allowing for the accurate determination of the appropriate slaughtering time to ensure food safety. These results highlight the method's precision, reliability, and potential application in routine monitoring of SAL residues in poultry tissues. Overall, the proposed approach offers a valuable analytical tool for food safety laboratories and supports ongoing efforts to ensure compliance with international residue regulations.