Abstract Dengue fever represents a major global health challenge and it is one of the most rapidly spreading mosquito-borne viral infections throughout tropical and sub-tropical regions of the world. The study aimed: To assess the awareness and practice of health team about dengue fever at Al-Hodeidah city. Subjects and methods: Descriptive research design was used in the study. Convenient sample was used. The current study included 370 divided into: - 124 physicians, 183 nurses, 33 lab technicians, and 30 midwives. Results: The study revealed that 57% of health team had fair awareness level about dengue fever toward dengue fever. Moreover, 60.1% of nurses & midwives had poor practices, 46.0% of physicians had fair practices, and 63.6% of lab- technicians had good practices. Conclusion: the study concluded that all health team had fair awareness toward dengue fever. While the physicians had fair practices the nurses and midwives had poor practices but the lab technician's practices were good. Recommendations: The current study recommended education programs should be organized for nurses and midwives regarding management and prevention of dengue fever.