CD10 is widely expressed in various tumor types and their surrounding cancer associated fibroblasts; and correlate withpoor prognosis. In present study CD10 immunohistochemical expression in stromal fibroblasts surrounding urothelialcarcinoma and its relationship with clinicopathological parameters were investigated.
Patients and Methods
Formalin-fixed paraffin embedded tissue sections from 106 urothelial cancer specimens werestained with CD10 antibody using immunohistochemistry technique. Expression of CD10 in the stromal cells was thenanalysed to evaluate its association with different clinicopathological variables.
Results
Stromal CD10 expression was significantly associated with each of high grade (P<0.001), invasive tumor,advanced stage (P<0.001), squamous differentiation of tumor cells (P=0.02) and papillary architecture (P<0.001). Therewas no significant association between stromal CD10 expression and, and patient age, sex and tumor size.
Conclusion
Increased expression of CD10 in cancer associated fibroblasts was strongly correlated with tumorprogression and invasion
CD44 is an epithelial-mesenchymal transition (EMT) surface receptor that regulates the interactivity between the cells and the extracellular matrix, thereby promoting cell migration. The epidermal growth factor receptor (EGFR) family is a trans-membrane kinase-related protein. It regulates cell adhesion proteins, which may promote cell proliferation and invasiveness. Mesenchymal epithelial transition (MET) is another EMT receptor that stimulates cell proliferation, invasion, survival, and angiogenesis. This study aimed to evaluate the prognostic impact of CD44, EGFR expressions, and MET gene amplification in epithelial ovarian cancer (EOC).
This is a retrospective cohort study, including 85 cases of EOC. CD44 and EGFR expressions were evaluated in both epithelial and stromal cells by immunohistochemistry. Tumor cells also underwent a cytogenetic analysis using fluorescent in situ hybridization (FISH) to detect MET gene amplification.
High CD44 expression in tumors was significantly associated with serous subtypes (P=0.001), peritoneal deposits (P=0.002), and advanced stage (P=0.002). EGFR high tumor expression demonstrated a significant association with lymph node metastasis (P=0.038) and the advanced stage of EOC (P=0.016). Increased copy number of the MET gene was significantly associated with partial therapy response (P=0.030). CD44 and EGFR tumor high expression was associated with poor overall survival (OS). In addition, MET gene gain in tumors was associated with a shorter OS (P=0.000).
EMT biomarkers (CD44 and MET) and EGFR expression in EOC are independent prognostic factors for OS. MET gene increase copy number was detected in cases of serous neoplasm and associated with poor survival and minimal therapy response.
CD10 is expressed in urothelial carcinoma cells and cancer associated fibroblasts (CAF). In the current study, CD10 immunohistochemical staining (IHC) and CD10 mRNA expression in urothelial carcinoma of bladder (UCB) were assessed, and its relationship with tumor progression and prognosis was investigated.
In this study, 106 formalin fixed paraffin-embedded (FFPE) tissue of UCB, obtained through radical cystectomy specimen, and 10 matched normal tissue samples were included.CD10 expression was evaluated by immunohistochemistry and real time PCR techniques.
CD10 expression in tumor cells and associated stromal fibroblasts was significantly associated with high tumor grade and advanced stage. Significant correlation was found between CD10 tumor expression and lymphovascular invasion (LVI) (P<0.001) as well as perineural invasion (PNI). CD10 expression in stromal fibroblasts was significantly associated with squamous differentiation of tumor cells, lymph node metastasis (LNM), and tumor necrosis. Positive CD10 expression in both tumor cells and associated stromal fibroblasts was associated with shorter OS . CD10 mRNA was overexpressed in tumors in comparison with the matched normal tissues. CD10 mRNA was significantly higher in invasive tumor, advanced stage tumor, and high grade tumor. There was significant correlation between CD10 mRNA tumor expression and LVI, PNI, and tumor recurrence.
Increased expression of CD10 in the tumor and CAF was strongly correlated with tumor progression, invasion, metastasis, shorter OS, and RFS in urothelial carcinoma patients. CD10 mRNA showed significantly higher expression in tumor tissue than in matched normal tissue. CD10 mRNA was associated with depth of invasion, TNM stage, tumor grade, vascular tumor invasion, and tumor recurrence.
Background and objectives: Pediatric lymphoma is known to be associated with Epstein–Barr virus (EBV) infection. The objectives were to detect the frequency of EBV infection in pediatric lymphomas in our locality and to study its relationship with the clinicopathological characteristics of the patients.
Methods: In this descriptive cross-sectional study, we examined 78 consecutive cases of pediatric lymphomas for the presence of EBV in tumor cells by realtime Polymerase Chain Reaction on paraffin blocks for latent membrane protein-1 (LMP-1) over 3 years (January 2012 –January 2015). We collected data of the patients with pathology-proven primary lymphoma including age, sex, histologic subtype, and risk stratification.
Results: The most common subtype of pediatric lymphomas was NHL (52/78, 66.5%), while HL was diagnosed in 33.5%. EBV LMP-1 gene detection was found in 38.5% of HL, and 28.8% of NHL cases. EBV infection was significantly related to age, gender, and histological subtype in NHL cases; however, it was related to age only in those with HL. The overall difference in EBV LMP-1 gene detection was statistically significant regarding the age at presentation (P < 0.001).
Conclusion: The frequency of EBV infection in pediatric lymphomas in our locality is higher compared to Western countries, but it is lower than in endemic areas. Younger age at diagnosis was the most significant factor associated with EBV infection.
Background
Serine protease inhibitor kazal type 1 (SPINK1) was highly expressed in many cancer types and in colon,SPINK1 was involved in inflammation-induced colon cancer, proliferation and carcinogenesis. In this study, SPINK1 proteinexpression in colorectal cancer specimens was evaluated and correlated its expression with clinicopathological variables.
Material and methods
Formalin-fixed paraffin embedded tissue sections from 87 colorectal cancer specimens werestained with SPINK1 antibody using immunohistochemistry technique. Expression of SPINK1 was then analysed to evaluate itsassociation with clinicopathological variables.
Results
High SPINK1 protein expression was detected in 75 (86.2%) of CRC specimens and was significantly associated withlymph-vascular invasion (p < 0.001), and lymph node metastasis (p = 0.005), and advanced clinical stage (p= 0.003).
Conclusion
High SPINK1 protein expression is associated with lymph-vascular invasion and lymph node metastasis incolorectal cancer suggesting its role in invasion and metastases
The present work focused on the detection of immunoglobulins IgG, IgA, IgM and C3 complement in a wide variety of thyroid diseases as well as in normal thyroid tissue to evaluate the immunologic nature of these lesions. The study was encountered on 111 thyroid specimens selected from the biopsies received at the Pathology Laboratory over a period of 5 years. Formalin fixed paraffin embedded sections were prepared and stained by H and E and immunoperoxidase technique. IgG, IgA and IgM were detected in varying degrees in all types of thyroid lesions and also in normal thyroid tissue. While, positivity for C3 complement was detected in [6/10] cases of toxic nodular goiter, all cases of cretinism with goiter 8, in 11 out of 13 cases of Graves disease, in 14 out of 19 cases of thyroiditis and in 10 out of 16 cases of papillary carcinoma of the thyroid gland. However, no C3 complement could be detected in normal thyroid tissue, in cases of simple goiter, in all cases of thyroid adenoma and in all cases of follicular, anaplastic and medullary carcinoma examined. Results indicated that immunoglobulins are not predictors for diagnosis of autoimmunity in thyroid diseases, but are best considered as indicators for the gland activity. However, C3 and immunoglobulins may be used as useful markers for the diagnosis of papillary carcinoma and its follicular variant from follicular carcinoma
This work was done to study the variant histopathological pictures of the endometrium of postmenopausal bleeding [PMB] patients. 150 endometrial specimens of PMB cases were received at the Gynecological Laboratory of Assiut University Hospital. They were routinely processed, stained by hematoxylin and eosin and examined. PMB causes were classified into organic and non-organic. The former was 41% while, the later was 59% of the cases. The most common organic cause of bleeding was endometrial hyperplasia [36%]. Proliferative endometrium was the commonest cause of non-organic PMB [31%]. Endometrial carcinoma was found in 4% of the cases. The majority of PMB patients were in age ranging from 45 to 50 years [55%]
Do you have any questions? 
