Ultrasound guided pleural brushing: anew method for obtaining pleural specimen in malignant effusion

Research Abstract
Chest Department,AssiutUniversity,Assiut,Egypt Abstract Purpose: Encouraging positive diagnosticyieldsin malignantpleuraleffusion couldbeobtainedbypleuralbrushingperformedthroughtwotechniques,thefirstwasclosedandthesecondwasthoracoscopic.Untilnowtheultrasoundguidedpleuralbrushingisnotincludedwithinthesetechniquesanditsdiagnosticyield therefore is notevaluated.Sotheaim of thisstudywasto evaluatethe diagnosticyield of thisprocedureanditscontributionsasatechniquenotusedpreviouslyintheinterventionalpulmonologypracticetoobtainpleuralspecimenforcytologicalexaminationinmalignantpleuraleffusion.Methods:ThisprospectiveinterventionalstudywasconductedintheChestDepartment–AssiutUniversityHospitalduringtheperiod from July2014to September2015.Patientswhohadhighlysuspiciousmalignantpleural effusion(clinical,radiological,andlaboratory)werehospitalizedandenrolledinthisstudy.Patientswithbleedingtendencyorcoagulationprofileabnormalitieswereexcludedfromthestudy.Patientswerealsoexcludedfromthisstudyiftheetiologyofeffusionwasprovedtobebenign.Informedwrittenconsentwasobtainedfromallpatients.Theequipmentusedinourstudywereultrasoundapparatus(ALOKA–Prosound–SSD–3500SV),biopsyforceps(KARL–STORZ–Germany10329L–BS),thebronchoscopiccleaningbrush(PENTAX CS6002SN) trocar andcannula of Cope’sneedle and the semirigidthoracoscope(LTF; Olympus;Tokyo,Japan).Thoracentesis,pleuralbrushingandbiopsyforcepsofthepleurawereperformedforallenrolledpatientsintheultrasoundunitoftheChestDepartmentwhilethoracoscopywasdoneintheendoscopyunitonlyforpatientsinwhomthediagnosiscouldnotbeachievedbytheseprocedures.Results:Among22patientswhowerefinallydocumentedtohavemalignancy,theultrasoundguidedpleuralbrushingprovideddiagnosisin9(41%)/22cases,itwasexclusivelydiagnosticin3patients.Interestingly,theyieldof thisprocedurehad its contributionsregardingthefinalpathologicaldiagnosisof ourcases,itcouldaugmentthepositiveyieldtobe55%insteadof41%(forpleuralfluidcytologyalone),82%insteadof68%(forbiopsyforcepsalone)and86%insteadof72%(forbothfluidcytologyandforcepsbiopsy).Therecordedcomplicationsinourstudywereminimalandnotassociatedwithanymortality.Conclusions:Ultrasound-guidedpleuralbrushingisanewmethodforobtainingpleuralspecimens.Itisasimpleandrelativelysafeprocedure.Thistechniqueprovidesadditionaldiagnosticyieldin malignantpleuraleffusion.Werecommend itbesideothersin our diagnosticpracticeforsuspiciousmalignanteffusionespeciallywhenthoracoscopy is notavailable.
Research Authors
GamalAgmy
,YousefAhmed
,AlaaTHassan
Research Department
Department of Chest Diseases
Research Journal
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Member
Alaa El-Din Thabet Hasan Abdel-mabboud
Research Pages
NULL
Research Publisher
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Rank
1
Research Vol
NULL
Research Website
NULL
Research Year
2016

The impact of smoking on inflammatory biomarkers in patients with chronic obstructive pulmonary disease.
accepted April 2016 EgyptianJournalofChestDiseasesandTuberculosis

Research Abstract
ABSTRACT Background:Chronicobstructivepulmonarydisease(COPD)isachronicprogressiveinflammatorydiseasecharacterizedbylimitationsairflowthatisnotfullyreversible(42).ThepathophysiologyofCOPDisnotcompletelyunderstood. Cigarettesmoking is amajorriskfactorforchronicobstructivepulmonarydisease(COPD).ElevatedCRPhasbeenincreasinglyusedasasurrogatemarkerofsystemicinflammationindiverseconditions.TNF-α,apowerfulpro-inflammatorycytokineprimarilyproducedbyactivatedmacrophages,isthoughttoacriticalroleinthepathogenesisofCOPD[13,14]. Theaimofthework:toevaluatetheimpactofsmokingoninflamatorybiomarkersandrelationsbetweenthesebiomarkersandthedeclineoflungfunctioninCOPDpatients. Methods:Thiscase–controlobservationalprospectivestudywasconductedonfifty-eightclinicallystableCOPDpatients(26non-smokersand32current smokers;at differentstagesrangedfrommildtoverysevere),theirmeanage53.1±14.25and53.9±5.95yearsrespectively),recruitedfromChestDepartment,AssiutUniversityHospitals.AllpatientsmettheGlobalInitiativeforObstructiveLungDisease(GOLD)[4].Allparticipantsweresubjectedtothoroughhistorytaking,fullclinicalexamination,anthropometricmeasurementswithspirometryandchest X-ray.Peripheralhemogram,liverfunctiontests,kidneyfunctiontests,highsensitivityC-reactiveprotein(hsCRP)andserumlevelofTNF-αweremeasuredforbothpatientsandcontrols. Results:Theconcentrationsofcirculatinghs-CRPandTNF-α,werehighlysignificantlyelevatedinpatientswithCOPDincomparisontothecontrolgroup(3.74±0.2vs.1.30±0.14forhs-CRP;33.88±5.97vs.8.79±0.57forTNF-αwithp0.0001foreach)andthelevelsofmeasuredTNF-αweresignificantlyincreasedwiththeincreaseddegreeandseverityofCOPDandincreasedseverityofsmokingstatus.RegardingthesmokingstatusofCOPDpatients,therewasahighlysignificantlydifferencefor the measuredTNF-α(53.74±9.52versus12.73±1.20withp0.0001)withnosignificantdifferenceforthemeasuredhs-CRP(3.87±0.29versus 3.58±0.27withp˃0.05).Interestingly,therewere significant negativecorrelationsbetweenthelevelsofTNF-αand hs-CRP,andFEV1instagesII,III,IVofCOPD. Conclusions:Thecirculatinglevelsoftheinflammatorymarkershs-CRPandTNF-alphaaresignificantlyelevatedinpatientswithstableCOPDandthesebiomarkerscouldbeusedaspredictorfactorsforseverityofinflammationinCOPDpatients..Longitudinalstudiesevaluatingtheeffectsofsmokingcessationonbronchialandsystemicinflammationareneededtoallowbetterunderstandingoftheserelationshipsandtheirconsequences. Keywords:COPD,FEV1,inflammatorymarkers;hs-CRPandTNF-alpha.
Research Authors
YasserA.Korani
¹,AlaaT.Hassan¹
,EffatA.E.Tony²,
MadleenAdelA.Abdou
Research Department
Internal Medicine
Research Journal
EgyptianJournalofChestDiseasesandTuberculosis
Research Member
Effat Abdel-Hady Tony Ahmed
Research Pages
NULL
Research Publisher
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Rank
1
Research Vol
NULL
Research Website
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Year
2016

The impact of smoking on inflammatory biomarkers in patients with chronic obstructive pulmonary disease.
accepted April 2016 EgyptianJournalofChestDiseasesandTuberculosis

Research Abstract
ABSTRACT Background:Chronicobstructivepulmonarydisease(COPD)isachronicprogressiveinflammatorydiseasecharacterizedbylimitationsairflowthatisnotfullyreversible(42).ThepathophysiologyofCOPDisnotcompletelyunderstood. Cigarettesmoking is amajorriskfactorforchronicobstructivepulmonarydisease(COPD).ElevatedCRPhasbeenincreasinglyusedasasurrogatemarkerofsystemicinflammationindiverseconditions.TNF-α,apowerfulpro-inflammatorycytokineprimarilyproducedbyactivatedmacrophages,isthoughttoacriticalroleinthepathogenesisofCOPD[13,14]. Theaimofthework:toevaluatetheimpactofsmokingoninflamatorybiomarkersandrelationsbetweenthesebiomarkersandthedeclineoflungfunctioninCOPDpatients. Methods:Thiscase–controlobservationalprospectivestudywasconductedonfifty-eightclinicallystableCOPDpatients(26non-smokersand32current smokers;at differentstagesrangedfrommildtoverysevere),theirmeanage53.1±14.25and53.9±5.95yearsrespectively),recruitedfromChestDepartment,AssiutUniversityHospitals.AllpatientsmettheGlobalInitiativeforObstructiveLungDisease(GOLD)[4].Allparticipantsweresubjectedtothoroughhistorytaking,fullclinicalexamination,anthropometricmeasurementswithspirometryandchest X-ray.Peripheralhemogram,liverfunctiontests,kidneyfunctiontests,highsensitivityC-reactiveprotein(hsCRP)andserumlevelofTNF-αweremeasuredforbothpatientsandcontrols. Results:Theconcentrationsofcirculatinghs-CRPandTNF-α,werehighlysignificantlyelevatedinpatientswithCOPDincomparisontothecontrolgroup(3.74±0.2vs.1.30±0.14forhs-CRP;33.88±5.97vs.8.79±0.57forTNF-αwithp0.0001foreach)andthelevelsofmeasuredTNF-αweresignificantlyincreasedwiththeincreaseddegreeandseverityofCOPDandincreasedseverityofsmokingstatus.RegardingthesmokingstatusofCOPDpatients,therewasahighlysignificantlydifferencefor the measuredTNF-α(53.74±9.52versus12.73±1.20withp0.0001)withnosignificantdifferenceforthemeasuredhs-CRP(3.87±0.29versus 3.58±0.27withp˃0.05).Interestingly,therewere significant negativecorrelationsbetweenthelevelsofTNF-αand hs-CRP,andFEV1instagesII,III,IVofCOPD. Conclusions:Thecirculatinglevelsoftheinflammatorymarkershs-CRPandTNF-alphaaresignificantlyelevatedinpatientswithstableCOPDandthesebiomarkerscouldbeusedaspredictorfactorsforseverityofinflammationinCOPDpatients..Longitudinalstudiesevaluatingtheeffectsofsmokingcessationonbronchialandsystemicinflammationareneededtoallowbetterunderstandingoftheserelationshipsandtheirconsequences. Keywords:COPD,FEV1,inflammatorymarkers;hs-CRPandTNF-alpha.
Research Authors
YasserA.Korani
¹,AlaaT.Hassan¹
,EffatA.E.Tony²,
MadleenAdelA.Abdou
Research Department
Department of Clinical Pathology
Research Journal
EgyptianJournalofChestDiseasesandTuberculosis
Research Member
Madleen Adel Attia Abdou
Research Pages
NULL
Research Publisher
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Rank
1
Research Vol
NULL
Research Website
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Year
2016

The impact of smoking on inflammatory biomarkers in patients with chronic obstructive pulmonary disease.
accepted April 2016 EgyptianJournalofChestDiseasesandTuberculosis

Research Abstract
ABSTRACT Background:Chronicobstructivepulmonarydisease(COPD)isachronicprogressiveinflammatorydiseasecharacterizedbylimitationsairflowthatisnotfullyreversible(42).ThepathophysiologyofCOPDisnotcompletelyunderstood. Cigarettesmoking is amajorriskfactorforchronicobstructivepulmonarydisease(COPD).ElevatedCRPhasbeenincreasinglyusedasasurrogatemarkerofsystemicinflammationindiverseconditions.TNF-α,apowerfulpro-inflammatorycytokineprimarilyproducedbyactivatedmacrophages,isthoughttoacriticalroleinthepathogenesisofCOPD[13,14]. Theaimofthework:toevaluatetheimpactofsmokingoninflamatorybiomarkersandrelationsbetweenthesebiomarkersandthedeclineoflungfunctioninCOPDpatients. Methods:Thiscase–controlobservationalprospectivestudywasconductedonfifty-eightclinicallystableCOPDpatients(26non-smokersand32current smokers;at differentstagesrangedfrommildtoverysevere),theirmeanage53.1±14.25and53.9±5.95yearsrespectively),recruitedfromChestDepartment,AssiutUniversityHospitals.AllpatientsmettheGlobalInitiativeforObstructiveLungDisease(GOLD)[4].Allparticipantsweresubjectedtothoroughhistorytaking,fullclinicalexamination,anthropometricmeasurementswithspirometryandchest X-ray.Peripheralhemogram,liverfunctiontests,kidneyfunctiontests,highsensitivityC-reactiveprotein(hsCRP)andserumlevelofTNF-αweremeasuredforbothpatientsandcontrols. Results:Theconcentrationsofcirculatinghs-CRPandTNF-α,werehighlysignificantlyelevatedinpatientswithCOPDincomparisontothecontrolgroup(3.74±0.2vs.1.30±0.14forhs-CRP;33.88±5.97vs.8.79±0.57forTNF-αwithp0.0001foreach)andthelevelsofmeasuredTNF-αweresignificantlyincreasedwiththeincreaseddegreeandseverityofCOPDandincreasedseverityofsmokingstatus.RegardingthesmokingstatusofCOPDpatients,therewasahighlysignificantlydifferencefor the measuredTNF-α(53.74±9.52versus12.73±1.20withp0.0001)withnosignificantdifferenceforthemeasuredhs-CRP(3.87±0.29versus 3.58±0.27withp˃0.05).Interestingly,therewere significant negativecorrelationsbetweenthelevelsofTNF-αand hs-CRP,andFEV1instagesII,III,IVofCOPD. Conclusions:Thecirculatinglevelsoftheinflammatorymarkershs-CRPandTNF-alphaaresignificantlyelevatedinpatientswithstableCOPDandthesebiomarkerscouldbeusedaspredictorfactorsforseverityofinflammationinCOPDpatients..Longitudinalstudiesevaluatingtheeffectsofsmokingcessationonbronchialandsystemicinflammationareneededtoallowbetterunderstandingoftheserelationshipsandtheirconsequences. Keywords:COPD,FEV1,inflammatorymarkers;hs-CRPandTNF-alpha.
Research Authors
YasserA.Korani
¹,AlaaT.Hassan¹
,EffatA.E.Tony²,
MadleenAdelA.Abdou
Research Department
Department of Chest Diseases
Research Journal
EgyptianJournalofChestDiseasesandTuberculosis
Research Member
Yasser Ahmed Mohamed Mohamed
Research Pages
NULL
Research Publisher
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Rank
1
Research Vol
NULL
Research Website
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Year
2016

The impact of smoking on inflammatory biomarkers in patients with chronic obstructive pulmonary disease.
accepted April 2016 EgyptianJournalofChestDiseasesandTuberculosis

Research Abstract
ABSTRACT Background:Chronicobstructivepulmonarydisease(COPD)isachronicprogressiveinflammatorydiseasecharacterizedbylimitationsairflowthatisnotfullyreversible(42).ThepathophysiologyofCOPDisnotcompletelyunderstood. Cigarettesmoking is amajorriskfactorforchronicobstructivepulmonarydisease(COPD).ElevatedCRPhasbeenincreasinglyusedasasurrogatemarkerofsystemicinflammationindiverseconditions.TNF-α,apowerfulpro-inflammatorycytokineprimarilyproducedbyactivatedmacrophages,isthoughttoacriticalroleinthepathogenesisofCOPD[13,14]. Theaimofthework:toevaluatetheimpactofsmokingoninflamatorybiomarkersandrelationsbetweenthesebiomarkersandthedeclineoflungfunctioninCOPDpatients. Methods:Thiscase–controlobservationalprospectivestudywasconductedonfifty-eightclinicallystableCOPDpatients(26non-smokersand32current smokers;at differentstagesrangedfrommildtoverysevere),theirmeanage53.1±14.25and53.9±5.95yearsrespectively),recruitedfromChestDepartment,AssiutUniversityHospitals.AllpatientsmettheGlobalInitiativeforObstructiveLungDisease(GOLD)[4].Allparticipantsweresubjectedtothoroughhistorytaking,fullclinicalexamination,anthropometricmeasurementswithspirometryandchest X-ray.Peripheralhemogram,liverfunctiontests,kidneyfunctiontests,highsensitivityC-reactiveprotein(hsCRP)andserumlevelofTNF-αweremeasuredforbothpatientsandcontrols. Results:Theconcentrationsofcirculatinghs-CRPandTNF-α,werehighlysignificantlyelevatedinpatientswithCOPDincomparisontothecontrolgroup(3.74±0.2vs.1.30±0.14forhs-CRP;33.88±5.97vs.8.79±0.57forTNF-αwithp0.0001foreach)andthelevelsofmeasuredTNF-αweresignificantlyincreasedwiththeincreaseddegreeandseverityofCOPDandincreasedseverityofsmokingstatus.RegardingthesmokingstatusofCOPDpatients,therewasahighlysignificantlydifferencefor the measuredTNF-α(53.74±9.52versus12.73±1.20withp0.0001)withnosignificantdifferenceforthemeasuredhs-CRP(3.87±0.29versus 3.58±0.27withp˃0.05).Interestingly,therewere significant negativecorrelationsbetweenthelevelsofTNF-αand hs-CRP,andFEV1instagesII,III,IVofCOPD. Conclusions:Thecirculatinglevelsoftheinflammatorymarkershs-CRPandTNF-alphaaresignificantlyelevatedinpatientswithstableCOPDandthesebiomarkerscouldbeusedaspredictorfactorsforseverityofinflammationinCOPDpatients..Longitudinalstudiesevaluatingtheeffectsofsmokingcessationonbronchialandsystemicinflammationareneededtoallowbetterunderstandingoftheserelationshipsandtheirconsequences. Keywords:COPD,FEV1,inflammatorymarkers;hs-CRPandTNF-alpha.
Research Authors
YasserA.Korani
¹,AlaaT.Hassan¹
,EffatA.E.Tony²,
MadleenAdelA.Abdou
Research Department
Department of Chest Diseases
Research Journal
EgyptianJournalofChestDiseasesandTuberculosis
Research Member
Alaa El-Din Thabet Hasan Abdel-mabboud
Research Pages
NULL
Research Publisher
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Rank
1
Research Vol
NULL
Research Website
Egyptian Journal of Chest Diseases and Tuberculosis www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Year
2016

Role of continuous positive airway pressure in patients with combined sleep apnea syndrome without congestive heart failure

Research Abstract
AbstractContinuouspositiveairwaypressure(CPAP)isastandardtreatmentofmoderateandsevereobstructive sleepapnea syndrome.However, itseffect inpatients with coexistingobstructiveandcentralapneasiscontroversial. Objectives:TodeterminetheimmediateresponsetoCPAPincombinedobstructiveandcentralsleepapneapatientswithoutheartfailure. Methods:Thirty sevenconsecutivepatients with moderateandsevere coexisting obstructive andcentralapneas(combinedgroup)wereprospectivelyenrolledinthiscrosssectionalanalyticstudy.Allpatientsunderwentafullnight-attendedandafullnightCPAPtitrationpolysomnography.TitrationwasconsideredsuccessfulifAHI 10andthetitrationstudyincludedatleast15 min inREMstage. Results:OnCPAPtitration,thecombinedgroupshowedsignificantimprovementinsleepandrespiratorypolysomnographicparameters.MeanAHIwasreducedfrom71.9±30.3to 8.39±5.15(P=0.000).WhereasCPAPsignificantlyreducedthecentralapneaindexfrom 12.8±6.67to3.1±2.86(P=0.000),theresponsetocentraleventswasvariable(rangedfrom20%to100%).Overallresults,25(67.6%)hadsuccessfultitrationwithsignificantbetterresponseinfemalesthanmalestoCPAPthanmales(88.9%vs.60.7%,P=0.019). Conclusion:CPAPcanbeeffectiveincombinedobstructiveandcentralapneapatientswithoutheartfailurewithconsiderationofindividualvariability.AtrialofCPAPtitrationshouldbedoneinthosepatients. 2016TheEgyptianSocietyof ChestDiseasesandTuberculosis.Productionandhostingby ElsevierB.V.Thisisanopenaccessarticle under theCCBY-NC-ND license (http://creativecommons.org/licenses/by-nc-
Research Authors
ShereenFarghaly1,*,AlaaThabet2Chest
Research Department
Department of Chest Diseases
Research Journal
TheEgyptianSocietyofChestDiseasesandTuberculosis
EgyptianJournalofChestDiseasesandTuberculosis
Research Member
Alaa El-Din Thabet Hasan Abdel-mabboud
Research Pages
1-7
Research Publisher
www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Rank
1
Research Vol
NULL
Research Website
TheEgyptianSocietyofChestDiseasesandTuberculosis
Research Year
2016

Role of continuous positive airway pressure in patients with combined sleep apnea syndrome without congestive heart failure

Research Abstract
AbstractContinuouspositiveairwaypressure(CPAP)isastandardtreatmentofmoderateandsevereobstructive sleepapnea syndrome.However, itseffect inpatients with coexistingobstructiveandcentralapneasiscontroversial. Objectives:TodeterminetheimmediateresponsetoCPAPincombinedobstructiveandcentralsleepapneapatientswithoutheartfailure. Methods:Thirty sevenconsecutivepatients with moderateandsevere coexisting obstructive andcentralapneas(combinedgroup)wereprospectivelyenrolledinthiscrosssectionalanalyticstudy.Allpatientsunderwentafullnight-attendedandafullnightCPAPtitrationpolysomnography.TitrationwasconsideredsuccessfulifAHI 10andthetitrationstudyincludedatleast15 min inREMstage. Results:OnCPAPtitration,thecombinedgroupshowedsignificantimprovementinsleepandrespiratorypolysomnographicparameters.MeanAHIwasreducedfrom71.9±30.3to 8.39±5.15(P=0.000).WhereasCPAPsignificantlyreducedthecentralapneaindexfrom 12.8±6.67to3.1±2.86(P=0.000),theresponsetocentraleventswasvariable(rangedfrom20%to100%).Overallresults,25(67.6%)hadsuccessfultitrationwithsignificantbetterresponseinfemalesthanmalestoCPAPthanmales(88.9%vs.60.7%,P=0.019). Conclusion:CPAPcanbeeffectiveincombinedobstructiveandcentralapneapatientswithoutheartfailurewithconsiderationofindividualvariability.AtrialofCPAPtitrationshouldbedoneinthosepatients. 2016TheEgyptianSocietyof ChestDiseasesandTuberculosis.Productionandhostingby ElsevierB.V.Thisisanopenaccessarticle under theCCBY-NC-ND license (http://creativecommons.org/licenses/by-nc-
Research Authors
ShereenFarghaly1,*,AlaaThabet2Chest
Research Department
Department of Chest Diseases
Research Journal
TheEgyptianSocietyofChestDiseasesandTuberculosis
EgyptianJournalofChestDiseasesandTuberculosis
Research Member
Shereen Farghaly Gad Ahmed
Research Pages
1-7
Research Publisher
www.elsevier.com/locate/ejcdtwww.sciencedirect.com
Research Rank
1
Research Vol
NULL
Research Website
TheEgyptianSocietyofChestDiseasesandTuberculosis
Research Year
2016

Immunomodulatory Effects of Levofloxacin on Patients with Pneumonia in Assiut University Hospitals. THE EGYPTIAN JOURNAL OF IMMUNOLOGY
Vol. 22 (1), 2015 Page: 85-91

Research Abstract
Theimmunomodulatory effectsofantibioticscouldinfluencethedegreeofsystemicandlocalresponsestoinfection,soinvestigationoftheirintrinsicinfluenceonthehost’sinflammatoryresponseappearstobeessential.Fluoroquinolonesareknowntoexertmodulatoryactivityonimmuneresponsestomicrobialinfection.Howeverthemechanismofthisimmunmodulationhasnotbeenwellelucidated.Theaimofthework,istoassesstheimmunomodulatoryeffectsofalevofloxacin,throughexaminingitseffectontheconcentrationsoftumornecrosisfactorα(TNF-α)andInterleukin–10(IL-10)inserumofpneumonicpatients.Afterfollowinglocalresearchethicscommitteeapprovalandinformedconsent.Thisstudyincluded 40patientswithdifferenttypesofpneumonia,admittedtodepartmentofChestDiseases,FacultyofMedicine,AssiutUniversityHospitals,Egypt.Also,10healthyvolunteersservedasrandomizedcontrols.Bothpatientsandcontrolsreceivedlevofloxacin(750mgoncedailyfor10days).SerumlevelsofTNF-αandIL-10weremeasuredinpatientsandcontrolbeforeandafterlevofloxacinadministration(750mgoncedailyfor10days)usinghumanTNF–αandIL-10ELISAkitsrespectively.LevofloxacincausedastatisticallysignificantdecreaseinthemeanlevelofTNF-αinbothpatients(20.82±1.31pg/ml)(P0.009)andcontrolgroup(17.12±0.84pg/ml)(P0.004).Incontrast,therewasstatisticallysignificantincrease(P0.000)inthemeanlevelofIL-10inpatients (61.75±2.85pg/ml)whilestatisticallysignificantdecrease(P0.005)incontrolgroup(28.57±1.37pg/ml).Inconclusion,ourstudydemonstratesthattreatmentwithlevofloxacinaffectsproductionofTNF-αasapro-inflammatorycytokineandIL-10asananti-inflammatorycytokineswhichmayprovide additionalbenefitsintreatmentof respiratorytract infectionsthatare independentofitsantibacterialproperties.
Research Authors
1MohamedS.Badari,
1ShereinG.Elgendy,
1AsmaaS.Mohamed,
2Alaa
T.Hassan
Research Department
Department of Microbiology and Immunology
Research Journal
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Member
Mohamed Saad Badary Ali
Research Pages
85-91
Research Publisher
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Rank
2
Research Vol
Vol. 22 (1),
Research Website
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Year
2015

Immunomodulatory Effects of Levofloxacin on Patients with Pneumonia in Assiut University Hospitals. THE EGYPTIAN JOURNAL OF IMMUNOLOGY
Vol. 22 (1), 2015 Page: 85-91

Research Abstract
Theimmunomodulatory effectsofantibioticscouldinfluencethedegreeofsystemicandlocalresponsestoinfection,soinvestigationoftheirintrinsicinfluenceonthehost’sinflammatoryresponseappearstobeessential.Fluoroquinolonesareknowntoexertmodulatoryactivityonimmuneresponsestomicrobialinfection.Howeverthemechanismofthisimmunmodulationhasnotbeenwellelucidated.Theaimofthework,istoassesstheimmunomodulatoryeffectsofalevofloxacin,throughexaminingitseffectontheconcentrationsoftumornecrosisfactorα(TNF-α)andInterleukin–10(IL-10)inserumofpneumonicpatients.Afterfollowinglocalresearchethicscommitteeapprovalandinformedconsent.Thisstudyincluded 40patientswithdifferenttypesofpneumonia,admittedtodepartmentofChestDiseases,FacultyofMedicine,AssiutUniversityHospitals,Egypt.Also,10healthyvolunteersservedasrandomizedcontrols.Bothpatientsandcontrolsreceivedlevofloxacin(750mgoncedailyfor10days).SerumlevelsofTNF-αandIL-10weremeasuredinpatientsandcontrolbeforeandafterlevofloxacinadministration(750mgoncedailyfor10days)usinghumanTNF–αandIL-10ELISAkitsrespectively.LevofloxacincausedastatisticallysignificantdecreaseinthemeanlevelofTNF-αinbothpatients(20.82±1.31pg/ml)(P0.009)andcontrolgroup(17.12±0.84pg/ml)(P0.004).Incontrast,therewasstatisticallysignificantincrease(P0.000)inthemeanlevelofIL-10inpatients (61.75±2.85pg/ml)whilestatisticallysignificantdecrease(P0.005)incontrolgroup(28.57±1.37pg/ml).Inconclusion,ourstudydemonstratesthattreatmentwithlevofloxacinaffectsproductionofTNF-αasapro-inflammatorycytokineandIL-10asananti-inflammatorycytokineswhichmayprovide additionalbenefitsintreatmentof respiratorytract infectionsthatare independentofitsantibacterialproperties.
Research Authors
1MohamedS.Badari,
1ShereinG.Elgendy,
1AsmaaS.Mohamed,
2Alaa
T.Hassan
Research Department
Department of Microbiology and Immunology
Research Journal
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Member
Shereen Gamal El-Din Abdel-Fattah Mostafa
Research Pages
85-91
Research Publisher
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Rank
2
Research Vol
Vol. 22 (1),
Research Website
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Year
2015

Immunomodulatory Effects of Levofloxacin on Patients with Pneumonia in Assiut University Hospitals. THE EGYPTIAN JOURNAL OF IMMUNOLOGY
Vol. 22 (1), 2015 Page: 85-91

Research Abstract
Theimmunomodulatory effectsofantibioticscouldinfluencethedegreeofsystemicandlocalresponsestoinfection,soinvestigationoftheirintrinsicinfluenceonthehost’sinflammatoryresponseappearstobeessential.Fluoroquinolonesareknowntoexertmodulatoryactivityonimmuneresponsestomicrobialinfection.Howeverthemechanismofthisimmunmodulationhasnotbeenwellelucidated.Theaimofthework,istoassesstheimmunomodulatoryeffectsofalevofloxacin,throughexaminingitseffectontheconcentrationsoftumornecrosisfactorα(TNF-α)andInterleukin–10(IL-10)inserumofpneumonicpatients.Afterfollowinglocalresearchethicscommitteeapprovalandinformedconsent.Thisstudyincluded 40patientswithdifferenttypesofpneumonia,admittedtodepartmentofChestDiseases,FacultyofMedicine,AssiutUniversityHospitals,Egypt.Also,10healthyvolunteersservedasrandomizedcontrols.Bothpatientsandcontrolsreceivedlevofloxacin(750mgoncedailyfor10days).SerumlevelsofTNF-αandIL-10weremeasuredinpatientsandcontrolbeforeandafterlevofloxacinadministration(750mgoncedailyfor10days)usinghumanTNF–αandIL-10ELISAkitsrespectively.LevofloxacincausedastatisticallysignificantdecreaseinthemeanlevelofTNF-αinbothpatients(20.82±1.31pg/ml)(P0.009)andcontrolgroup(17.12±0.84pg/ml)(P0.004).Incontrast,therewasstatisticallysignificantincrease(P0.000)inthemeanlevelofIL-10inpatients (61.75±2.85pg/ml)whilestatisticallysignificantdecrease(P0.005)incontrolgroup(28.57±1.37pg/ml).Inconclusion,ourstudydemonstratesthattreatmentwithlevofloxacinaffectsproductionofTNF-αasapro-inflammatorycytokineandIL-10asananti-inflammatorycytokineswhichmayprovide additionalbenefitsintreatmentof respiratorytract infectionsthatare independentofitsantibacterialproperties.
Research Authors
1MohamedS.Badari,
1ShereinG.Elgendy,
1AsmaaS.Mohamed,
2Alaa
T.Hassan
Research Department
Department of Microbiology and Immunology
Research Journal
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Member
Asmaa Salah Mohammed Hussein
Research Pages
85-91
Research Publisher
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Rank
2
Research Vol
Vol. 22 (1),
Research Website
EGYPTIAN JOURNAL OF IMMUNOLOGY
Research Year
2015
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