ABSTRACT: Six isolates of Sclerotium cepivorum Berk were isolated from naturally infected garlic plants collected from different localities of El-minya, Assiut and Sohag Governorates. Pathogenicity tests indicated that isolates No.2, 3 and 6 were highly pathogenic to garlic as compared with isolates No. 1, 4 and 5. Protein of six isolates of S. cepivorum was compared by polyacrylamide gel electrophoresis (PAGE) and sodium dodecyel sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). Protein profiles separated by PAGE, isolate No. 1 showed the highest number of bands (20 bands), while isolate No. 4 showed the lowest number of bands (15 bands). The number of bands of other isolates was 16 or 17 bands. Protein profiles separated by SDS-PAGE, isolate No. 5 showed the highest number of bands (19 bands) while isolate No. 3 showed the lowest number of bands (6 bands). The other isolates showed a number of bands ranged from 13 to 17 bands. On the basis of electrophoretic dissimilarities among protein banding paterns, isolates were grouped by claster analysis and the results were expressed as phenograms. Grouping the isolates based on PAGE analysis was associated with geographic of isolates, however, grouping the isolates based on SDS-PAGE was associated with virulence of isolates.

ABSTRACT: Six isolates of Sclerotium cepivorum Berk were isolated from naturally infected garlic plants collected from different localities of El-minya, Assiut and Sohag Governorates. Pathogenicity tests indicated that isolates No.2, 3 and 6 were highly pathogenic to garlic as compared with isolates No. 1, 4 and 5. Protein of six isolates of S. cepivorum was compared by polyacrylamide gel electrophoresis (PAGE) and sodium dodecyel sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). Protein profiles separated by PAGE, isolate No. 1 showed the highest number of bands (20 bands), while isolate No. 4 showed the lowest number of bands (15 bands). The number of bands of other isolates was 16 or 17 bands. Protein profiles separated by SDS-PAGE, isolate No. 5 showed the highest number of bands (19 bands) while isolate No. 3 showed the lowest number of bands (6 bands). The other isolates showed a number of bands ranged from 13 to 17 bands. On the basis of electrophoretic dissimilarities among protein banding paterns, isolates were grouped by claster analysis and the results were expressed as phenograms. Grouping the isolates based on PAGE analysis was associated with geographic of isolates, however, grouping the isolates based on SDS-PAGE was associated with virulence of isolates.

ABSTRACT: Six isolates of Sclerotium cepivorum Berk were isolated from naturally infected garlic plants collected from different localities of El-minya, Assiut and Sohag Governorates. Pathogenicity tests indicated that isolates No.2, 3 and 6 were highly pathogenic to garlic as compared with isolates No. 1, 4 and 5. Protein of six isolates of S. cepivorum was compared by polyacrylamide gel electrophoresis (PAGE) and sodium dodecyel sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). Protein profiles separated by PAGE, isolate No. 1 showed the highest number of bands (20 bands), while isolate No. 4 showed the lowest number of bands (15 bands). The number of bands of other isolates was 16 or 17 bands. Protein profiles separated by SDS-PAGE, isolate No. 5 showed the highest number of bands (19 bands) while isolate No. 3 showed the lowest number of bands (6 bands). The other isolates showed a number of bands ranged from 13 to 17 bands. On the basis of electrophoretic dissimilarities among protein banding paterns, isolates were grouped by claster analysis and the results were expressed as phenograms. Grouping the isolates based on PAGE analysis was associated with geographic of isolates, however, grouping the isolates based on SDS-PAGE was associated with virulence of isolates.

The dytiscid beetle, Cybister fimbriolatus (Say), (Coleoptera: Dytiscidae), found in the temporary pools in a village of Assiut, was noted to predate on the larvae and pupae of Culex pipiens L. (Diptera: Culicidae). The daily consumption of the beetle larvae C. fimbriolatus on the 2nd and 4th instar larvae and pupae of Culex pipiens was recorded .First larva of C. fimbriolatus consumed 1.33 to 19.00 2nd instar larvae/day, 2.00 to 15.67 4th instar larvae/day, and 6.00 to 18.67 pupae/day in average mosquito. The second instar larva of C. fimbriolatus consumed from 13.00 to 23.33 2nd instar larvae/day, 8.67 to 19.33 4th instar larvae/day and 9.33 to 19.67 pupae/day. The third larva of C. fimbriolatus consumed 13.67 to 24.33 2nd instar larvae/day, 10.00 to 20.67 4th instar larvae/day and 1.33 to 24.67 pupae/day. The last result confirmed that the third instar larvae of C. fimbriolatus played a bigger role than the first larvae and second larvae. Generally, larvae of the dytiscid beetle C. fimbriolatus proved to be an efficient predator of mosquito immature and useful in bio-control of Culex pipiens L.

The current study focused on certain aquatic arthropods extracted from some ponds located at El-Ghorieb area during two years starting from March, 2011. In the present study, twenty species of aquatic arthropods pertaining to six orders (i.e., Diptera, Hemiptera, Coleoptera, Odonata, Cladocera and Acari) were recorded from some water pools. All the aquatic species increase to enumerate through the spring season and the least numbers were recorded during winter, autumn and summer were inbetween. The occurrence percentage of each species inside its own order was also calculated. The study showed that the highest proportion of mosquito species with an average ratio of 62.29 and the least was with Odonata within average of 3.6.

Two spinosyn compounds, spinosad 12% SC and spinetoram 12% SC were evaluated against housefly under laboratory and field conditions. In field study, the two compounds showed high reduction in fly population especially at the highest rate used (2.4 ml/m2). According to LT50 value spinosad showed relatively higher activity than spinetoram. This indicated that females were more tolerant two spinosyn products than males. In laboratory study the toxicity of spinosad against 1st and 3rd instars was higher than spinetoram. The third instar larvae were more tolerant to the two compounds, than the first instar larvae. Against pupae, spinosad showed slightly high toxicity as compared with spinetoram.

The present study was designed and compares the efficacy of three Pesticides against the buffaloes lice (Haematopinus tuberculatus) infesting buffalo in the farmer houses in Sohag Governorate .The pesticides which treated were Malathion 57%, Diazinon15% and Diazinon 60% EC at concentration of ml/ liter water against lice. The pesticides were spray on all treatment and the animal examined was after 24 hrs till 45 days. The decrease or increase of the population at buffalo lice was estimated and comparison between the three pesticide. Diazinon 60% showed variable toxicity to animal parasites. According to the percentage of mortality recorded after24 hrs till 45 days followed by Malathion 57% with the same concentrations. The late efficiency was Diazinon 15%.This may be due to the low of the active ingredient in Diazinon 15% compared with Diazinon 60%.In general we can use Diazinon 60% with 1cm/liter water as spray on the animal body to control the buffalo lice.

The Ectoparasites arthropod of rodent infesting animals and human have gained much attention in Assiut. Rodent species in both old land and new reclaimed land was infested with various species from arthropods such as mites and ticks (Acari) and lice, fleas (Insecta), from this studied found that three species of mites Amerosieus sp., Hypoaspis koseii, Cheyletus zaheri and one species of hard tick was Amblyomma sp., infested rodent species which captured from the study areas. The study of ectoparasite from insecta show that three species of fleas were Xenopsylla cheopis, Leptopsylla segnis and Pulex irritans and one of lice was Polyp lax spinulosa infested the captured rodent species. In this study we found that 6 species (1 sucking lice, 2 fleas,l tick and 2 mites) of Ectoparasites arthropod infesting rodent species and different location. The Faculty of Agriculture farm ranked the first in high recorded number of Ectoparasites arthropod of rodent species while the Arab El- Awamer farm low recorded number of Ectoparasites arthropod of rodent species.

The present study was designed to determine the effect of seven chemicals used to control mange mite on sheep. These chemicals were (1) Mange cides 10%, (2) Tincture idone 4%, (3) Noromectin 1.0% used once a month, (4) Noromectin 1.0% used twice a month, (5) Mange cides + skin recovery, (5) Noromectin 1.0% injection + Mange cides and (7) Mange cides + skin recovery + Noromectin 1.0% treated twice a month. Sheep infected with scabies were responded to the healing of the disease when treated with the compounds mentioned above (i.e., Mange cide 10%; Tincture idone 4%; Norometcin 1.0% once a month and Noromectin 1.0% twice a month) are just 45, 45, 30, and 20 days, respectively.

Canola plantations are considered as an important r eservoir of several arthropod pests and natural ene mies. Two canola cultivars (Serw and Bactol) were cultivated during 2011/2012 growing season at Assiut Governora te. Sixteen arthropod species belonging to 12 families and 6 orders, rather than the predatory true spider were recorded. The collected species were divided into: 7 phytopahgous; 5 predators; 2 parasitoids and 2 be neficial species. The cabbage aphid Brevicoryne brassicae L. ; the peach aphid Myzus persicae (Sulz.) and their parasitoids Diaeretiella rapae (McIntoch) and Praon nicans (Mac kauer) in addition to thrips, Thrips tabaci Lind. c onstituted the most frequent, dominant and abundant species inhabiting canola plantations. The phytophagous group constituted more than 85%, while the predatory one's constituted less than 14%. The abovementioned species were found to be active from the last week of February till harvesting after (April 9). Concerning the aphi d parasitism, the first appearance of aphid parasitized mummies b y D. rapae and/or P. nicans was recorded in the sec ond half of March. The greatest parasitism percentage was recor ded on April 9 with an average of 79.5% and 77.58% on Serw and Bactol cultivars, respectively. Both of the tested cultivars were appeared as susceptible (S) to o nion Thrips T. tabaci and showed different resistance categories t o aphid species. This study needs more attention by plant breeders to transfer genes responsible for resistan ce to the newly produced and/or improved cultivars.