The goals of the present study are to identify the causative microorganism of CSN in the Arabian camels and to evaluate the effect of CSN with or without trypanosomosis on the health status of camels. Out of the examined cases, 10 camels showed clinical signs of CSN, with or without trypanosomosis. The following samples were collected; sterile bacteriological swabs from skin necrosis area, whole blood samples for hematological analysis and for diagnosis of trypanosomosis, and serum samples for measuring lipid peroxidation product (Malondialdehyde, MDA).The bacteriological examination of collected swabs from dermal lesion of CSN revealed that Staphylococcus aureus was the predominant bacterial isolate alone in 6 cases and coupled with other bacteria in the remained 4 cases, coupled with coagulase negative staphylococci in 3 cases and coupled with Streptococcus agalactiae in one case. Trypanosoma evansi infection was identified using polymerase chain reaction in 5 camels that had CSN. MDA showed significant increase in camels affected with CSN, whether associated or not associated with trypanosomosis and when compared with control healthy camels. The current study revealed that Staphylococcus aureus was the predominant bacterial isolate, camels may be infected with both trypanosomosis and CSN, lipid peroxidation products increased in the blood of camels with CSN and it is recommended to supply camels with antioxidants to overcome the deterioration in blood antioxidants status. Key words: camel, trypanosoma, MDA

The goals of the present study are to identify the causative microorganism of CSN in the Arabian camels and to evaluate the effect of CSN with or without trypanosomosis on the health status of camels. Out of the examined cases, 10 camels showed clinical signs of CSN, with or without trypanosomosis. The following samples were collected; sterile bacteriological swabs from skin necrosis area, whole blood samples for hematological analysis and for diagnosis of trypanosomosis, and serum samples for measuring lipid peroxidation product (Malondialdehyde, MDA).The bacteriological examination of collected swabs from dermal lesion of CSN revealed that Staphylococcus aureus was the predominant bacterial isolate alone in 6 cases and coupled with other bacteria in the remained 4 cases, coupled with coagulase negative staphylococci in 3 cases and coupled with Streptococcus agalactiae in one case. Trypanosoma evansi infection was identified using polymerase chain reaction in 5 camels that had CSN. MDA showed significant increase in camels affected with CSN, whether associated or not associated with trypanosomosis and when compared with control healthy camels. The current study revealed that Staphylococcus aureus was the predominant bacterial isolate, camels may be infected with both trypanosomosis and CSN, lipid peroxidation products increased in the blood of camels with CSN and it is recommended to supply camels with antioxidants to overcome the deterioration in blood antioxidants status. Key words: camel, trypanosoma, MDA

A total number of 100 female buffaloes were subjected to study. Macroscopic sarcocystis were collected from the esophagus of buffaloes slaughtered in Mosha slaughterhouse (Mosha, Assiut Governorate, Egypt) during the period from February to June 2010. Part from the esophagus containing the sarcocystis was fixed in 10% formol saline and was processed for histopathological exam. Serum samples from all animals were subjected to ELISA for detection of antibody to sarcocystis. The prevalence of macroscopic sarcocystis was 23%. On the other hand, ELISA technique revealed that 94.44% of examined animals were infected with sarcocystis. The sensitivity of the macroscopic method was 27%, specificity was 100%, positive predictive value was 100%, and negative predictive value was 7.46%. Histopathological sections of infected muscles showed cross and longitudinal sections of sarcocystis with different shape and size. The current study revealed that macroscopic examination for detection of sarcocystis is insufficient. Animals must be subjected to ELISA test to ensure that the animals are free from the parasite. It is recommended to apply control measures for the source of infection at the area of study.

A total number of 100 female buffaloes were subjected to study. Macroscopic sarcocystis were collected from the esophagus of buffaloes slaughtered in Mosha slaughterhouse (Mosha, Assiut Governorate, Egypt) during the period from February to June 2010. Part from the esophagus containing the sarcocystis was fixed in 10% formol saline and was processed for histopathological exam. Serum samples from all animals were subjected to ELISA for detection of antibody to sarcocystis. The prevalence of macroscopic sarcocystis was 23%. On the other hand, ELISA technique revealed that 94.44% of examined animals were infected with sarcocystis. The sensitivity of the macroscopic method was 27%, specificity was 100%, positive predictive value was 100%, and negative predictive value was 7.46%. Histopathological sections of infected muscles showed cross and longitudinal sections of sarcocystis with different shape and size. The current study revealed that macroscopic examination for detection of sarcocystis is insufficient. Animals must be subjected to ELISA test to ensure that the animals are free from the parasite. It is recommended to apply control measures for the source of infection at the area of study.

A total number of 100 female buffaloes were subjected to study. Macroscopic sarcocystis were collected from the esophagus of buffaloes slaughtered in Mosha slaughterhouse (Mosha, Assiut Governorate, Egypt) during the period from February to June 2010. Part from the esophagus containing the sarcocystis was fixed in 10% formol saline and was processed for histopathological exam. Serum samples from all animals were subjected to ELISA for detection of antibody to sarcocystis. The prevalence of macroscopic sarcocystis was 23%. On the other hand, ELISA technique revealed that 94.44% of examined animals were infected with sarcocystis. The sensitivity of the macroscopic method was 27%, specificity was 100%, positive predictive value was 100%, and negative predictive value was 7.46%. Histopathological sections of infected muscles showed cross and longitudinal sections of sarcocystis with different shape and size. The current study revealed that macroscopic examination for detection of sarcocystis is insufficient. Animals must be subjected to ELISA test to ensure that the animals are free from the parasite. It is recommended to apply control measures for the source of infection at the area of study.