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The pesticides have different adverse impacts on different life stages of fish species with attempts to use dietary antioxidants to counteract their effects. So, the present study investigated the potential protective effects of vitamin E (50 mg/kg BW), tomato paste (in terms of 9 mg lycopene/kg BW) and their combination versus atrazine-induced changes in Clarias gariepinus. This species were exposed to sublethal doses of atrazine of 1.7 and 3.4 mg/L for 15 and 30 days. Atrazine significantly (P0.05) induced free radicals in serum constituents. It caused a significant (P0.05) increase in serum glucose, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and lipid peroxidation in liver. Addition of vitamin E and/or tomato paste to the diet of atrazine-treated fish improved the serum glucose, MCV, MCH and LPO levels in comparison to control fish. On the other hand, atrazine significantly (P0.05) led to decline in serum total protein, total lipid, blood hemoglobin, total erythrocytes count, packed cell volume, and mean corpuscular hemoglobin concentration.These atrazine-induced parameters were improved with the dietary supplemented tomato paste and/or vitamin E.

The present investigation aimed to determine the tox-icological effects of mercury chloride on biochemical parameters of the widely consumed African catfish, Clarias gariepinus. Adult specimens of Clarias garie-pinus were exposed to sublethal concentrations (0.04 and 0.12 ppm) of mercury chloride for 7, 14, 21 and 28 days. Empirical data of results obtained were subjected to statistical analysis of variance (ANOVA) to test the effects of mercury, selenium, vitamin E and exposure periods. The mean values of Na + , Urea, Creatinine, AST, ALT and ALP were significantly in-creased from the control values, while mean values of K + , Glucose and Triglyceride were significantly decreased from the control values. Selenium and Vitamin E supplementation play appositive role in detoxification of mercury toxicity specially the low dose. The results suggest that mercury chloride can negatively affect the physiology of fish. It was observ-ed that supplementation of selenium and vitamin E decreases the toxic effect of mercury.

The present investigation aimed to determine the tox-icological effects of mercury chloride on biochemical parameters of the widely consumed African catfish, Clarias gariepinus. Adult specimens of Clarias garie-pinus were exposed to sublethal concentrations (0.04 and 0.12 ppm) of mercury chloride for 7, 14, 21 and 28 days. Empirical data of results obtained were subjected to statistical analysis of variance (ANOVA) to test the effects of mercury, selenium, vitamin E and exposure periods. The mean values of Na + , Urea, Creatinine, AST, ALT and ALP were significantly in-creased from the control values, while mean values of K + , Glucose and Triglyceride were significantly decreased from the control values. Selenium and Vitamin E supplementation play appositive role in detoxification of mercury toxicity specially the low dose. The results suggest that mercury chloride can negatively affect the physiology of fish. It was observ-ed that supplementation of selenium and vitamin E decreases the toxic effect of mercury.

The pesticides have different adverse impacts on different life stages of fish species with attempts to use dietary antioxidants to counteract their effects. So, the present study investigated the potential protective effects of vitamin E (50 mg/kg BW), tomato paste (in terms of 9 mg lycopene/kg BW) and their combination versus atrazine-induced changes in Clarias gariepinus. This species were exposed to sublethal doses of atrazine of 1.7 and 3.4 mg/L for 15 and 30 days. Atrazine significantly (P0.05) induced free radicals in serum constituents. It caused a significant (P0.05) increase in serum glucose, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and lipid peroxidation in liver. Addition of vitamin E and/or tomato paste to the diet of atrazine-treated fish improved the serum glucose, MCV, MCH and LPO levels in comparison to control fish. On the other hand, atrazine significantly (P0.05) led to decline in serum total protein, total lipid, blood hemoglobin, total erythrocytes count, packed cell volume, and mean corpuscular hemoglobin concentration.These atrazine-induced parameters were improved with the dietary supplemented tomato paste and/or vitamin E.

The pesticides have different adverse impacts on different life stages of fish species with attempts to use dietary antioxidants to counteract their effects. So, the present study investigated the potential protective effects of vitamin E (50 mg/kg BW), tomato paste (in terms of 9 mg lycopene/kg BW) and their combination versus atrazine-induced changes in Clarias gariepinus. This species were exposed to sublethal doses of atrazine of 1.7 and 3.4 mg/L for 15 and 30 days. Atrazine significantly (P0.05) induced free radicals in serum constituents. It caused a significant (P0.05) increase in serum glucose, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and lipid peroxidation in liver. Addition of vitamin E and/or tomato paste to the diet of atrazine-treated fish improved the serum glucose, MCV, MCH and LPO levels in comparison to control fish. On the other hand, atrazine significantly (P0.05) led to decline in serum total protein, total lipid, blood hemoglobin, total erythrocytes count, packed cell volume, and mean corpuscular hemoglobin concentration.These atrazine-induced parameters were improved with the dietary supplemented tomato paste and/or vitamin E.