The present study investigated effects of environmental relevant concentrations of 4-nonylphenol (0.05
to 0.08 to 0.1 mg/l) on the reproduction and embryonic developmental stages of catfish (Clarias
gariepinus). To determine the effect of 4-nonylphenol on reproduction; catfish were exposed to three
concentrations of 4-nonylphenol in a flow-through-system during spawning period (some for one week
and other for two weeks). At an estimated 4-nonylphenol concentration the fertilization rate and
hatching rate were significantly decreased with 4-nonylphenol concentrations increasing while the
incubation period, the mortality rate and malformed embryos ratio were increased. Also, the
development of embryos and larvae was affected by 4-nonylphenol in terms of morphological changes
and histopathological alterations.