Chemical investigation of the n-hexane extract of the sponge Diacarnus megaspinorhabdosa has provided a series of norterpenes, including three new norditerpene cyclic peroxides and five new norsesterterpene peroxides together with four known norterpene peroxides: nuapapuin A methyl ester, epimuqubilin B, methyl-2-epinuapapuinoate and methyl diacarnoate A. The structures of the new compounds were established on the basis of one and two dimensional NMR spectroscopic studies ( H, C, COSY, HMQC, HMBC and ROESY) as well as on mass spectral analysis. The isolated compounds exhibited moderate (2–5µg) to strong toxicity (0.01–0.10µg) toward L5178Y (mouse lymphoma) and HeLa (human cervix carcinoma) while the same congeners showed weaker activity on the PC-12 (rat pheochromocytoma) cell line. Capon's empirical rules1 were extensively used in this study to derive the relative stereochemistry at C-2, C-3 and C-6.Following Horeau's procedure, the peroxide ring was cleaved to yield its diol congener onto which the advanced Mosher method was utilized to confirm the stereochemistry obtained from Capon's empirical rules.

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The chloroform soluble fraction of the methanolic extract of the aerial parts of Tanacetum santolinoides afforded six sesquiterpene lactones. Tanacetolide A (1) was isolated for the first time from a natural source, in addition to five known sesquiterpene lactones. The structures were established on the basis of extensive studies of their 1D and 2D (1H-1H COSY,HMQC, HMBC, HOHAHA and NOESY) NMR spectra and FAB mass spectral determinations. The compounds showed good cytotoxic activity when tested using the brine shrimp bioassay.