The growing demand for medicinal plants in herbal medicine highlights their therapeutic value, yet heavy
metal contamination, such as cobalt (Co), poses potential health risks. Cobalt’s dual role as an essential
micronutrient and a toxic pollutant necessitates a more profound understanding of its impact on medicinal
plants like Adhatoda vasica. We investigated how varying Co concentrations affect A. vasica shoot
growth, leaf anatomy, antioxidant enzyme activity, and secondary metabolite profiles. Additionally,
molecular docking was performed to assess the interaction of the metabolites with the skin cancer-related
protein anti-ssDNA antigen-binding fragment (PDB code: 1P7K). Low Co concentrations (50 µM)
enhanced shoot dry weight by 41.45%, while higher levels (100–1000 µM) reduced it by up to 66.86%.
Cobalt exposure increased hydrogen peroxide (H2O2) and lipoxygenase (LOX) activity, indicating
reactive oxygen species (ROS)-induced oxidative stress. Higher Co levels increased superoxide dismutase
(SOD), catalase (CAT), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO) but
decreased peroxidase (POD) and ascorbate peroxidase (APX) activity. HPLC-UV and GC-MS data
showed that Co altered the secondary metabolites of A. vasica, including phenolics, flavonoids, alkaloids,
and terpenoids, in both qualitative and quantitative ways. Molecular docking shows that naringin has a
higher binding affinity (-9.2 kcal/mol) to PDB: 1P7K than phenolics (-4.8 to -6.4 kcal/mol). Cobalt stress
significantly impacts A. vasica, altering its leaf structure, growth, and antioxidant defenses. These effects
extend to secondary metabolites in a dose-dependent manner.
These findings highlight the plant’s potential for Co tolerance and its metabolites’ therapeutic promise,
particularly naringin, for skin cancer applications.
Keywords Adhatoda vasica L., Antioxidant enzymes, Cobalt, Anatomy, Metabolite profiling, Molecular
docking