Abstract Background: Infection of the urinary tract attributed to the use of an indwelling urinary catheter is one of the most common infections acquired by patients at health care centers. Aim: The primary aim of this study was to assess effect of catheter care maintenance bundle on reducing the incidence of urinary tract infection among catheterized patients. Setting: The study was conducted in the neurological department, affiliated to Assuit University Hospital Methods: This study was designed as interventional prospective study on purposive nonprobability sixty adult patients (experimental and control) undergoing urinary catheterization were included in this study. Results: There was a positive correlation between catheter care maintenance bundle and occurrence of urinary tract infection (P 0.003). Conclusion: Implementation of catheter care maintenance bundle intervention resulted in a reduction in the incidence of urinary tract infection among catheterized patients. Recommendations: Application of the catheter care maintenance bundle at all the settings that deal with urinary catheterization to decrease the infection rate.