The objective of the study is to evaluate efficiency of in vitro isolation and myogenic differentiation of mesenchymal stem cells (MSCs) derived from adipose connective tissue (AD-MSCs), bone marrow (BM-MSCs), and skeletal muscle tissue (MC-MSCs). MSCs were isolated from adipose connective tissue, bone marrow, and skeletal muscle tissue of two adult 6-wk-old rats. Cultured MSCs were treated with 5-azacytidine (AZA) to induce myogenic differentiation. Isolated MSCs and differentiated cells were evaluated by immunocytochemistry (ICC), fluorescenceactivated cell sorting (FACS), PCR, and RT-PCR. ADMSCs showed the highest proliferation rate while BMMSCs had the lowest one. In ICC, isolated MSCs had strong CD90- and CD44-positive expression and negative expression of CD45, CD31, and CD34, while AZA-treated MSCs had strong positive desmin expression. In FACS analysis, AD-MSCs had the highest percentage of CD90- and CD44- positive-expressing cells (99% and 96%) followed by BMMSCs (97% and 94%) and MC-MSCs (92% and 91%).At 1 wk after incubation with AZA treatment, the peak of myogenin expression reached 93% in differentiated MCMSCs, 83.3% in BM-MSCs, and 77% in AD-MSCs. MSCs isolated from adipose connective tissue, bone marrow, and skeletal muscle tissue have the same morphology and phenotype, but AD-MSCs were the most easily accessible and had the highest rate of growth on cultivation and the highest percentage of stem cell marker expression. Moreover, although MC-MSCs showed the highest rate of myogenic differentiation potential and expression of myoblast markers, AD-MSCs and BM-MSCs still can be valuable alternatives. The differentiated myoblastic cells could be an available new choice for myoblastic auto-transplantation in regeneration medicine.

Purpose of review This review outlines the advantages and the differences of femtosecond laser-assisted cataract surgery (FLACS) following a coaxial or microincision cataract surgery phacoemulsification in the surgical outcome and greater control of cataract surgery. Recent findings FLACS offers minimal tissue damage and extreme precision during corneal incision creation, continuous circular capsulorhexis (CCC) and nuclear fragmentation. It also allows diminishing the mean average ultrasound power to emulsify the nucleus followed by a coaxial or a biaxial procedure. The impact of reduced phacoemulsification energy on the corneal endothelium is an interesting topic that is being investigated. Despite its benefits, this technology has relevant financial issues and a high learning curve. Summary FemtoMICS appears to be surgically and statistically more efficient than the FemtoCoaxial technique and Femtoincisions prove to be stable and do not change the corneal high order aberration significantly with favorable results of the triplanar configuration.

The appendix is a precious substitute that could be relied upon for compensation of extensive ureteral losses either alone or combined with bladder flaps without adding morbidity either to the patient or to the urinary tract in terms of electrolyte disturbances or mucous production in addition to preservation of functional integrity of the involved renal unit

PURPOSE: Analysis of the femtosecond laser refractive lens surgery corneal incision confi guration and corneal higher-order aberration (HOA) effect from the fi rst postoperative day. METHODS: High-resolution anterior segment optical coherence tomography was used to assess 20 eyes undergoing femtosecond laser refractive lens surgery with 2.2-mm minimal incision. The primary incision (triplanar) actual length, cord length, surface angle, surface irregularity, and regional pachymetry values and the secondary incision (uni-planar) length, angle, surface irregularity, and pachymetry values were analyzed. Hartmann-Shack aberrometer was used to assess corneal HOAs to correlate the effect. Assessment was done preoperatively and 1 month postoperatively. RESULTS: The actual length, cord length, and surface angle means for the primary incision in the fi rst postoperative day and month were 1.50 ± 0.1 and 1.47 ± 0.2 mm (P = .5), 1.41 ± 0.1 and 1.42 ± 0.2 mm (P = .8), and 27° ± 4° and 23° ± 5° (P = .07), respectively. The length and surface angle for the secondary incision in the fi rst postoperative day and month were 1.17 ± 0.01 and 1.04 ± 0.1 mm (P = .05) and 52° ± 3° and 42° ± 5° (P = .007). The regional pachymetry values for the primary and secondary incisions were signifi cantly increased in the fi rst postoperative day and then signifi cantly decreased after 1 month. All irregularities occurred in the posterior surface (endothelium): 2 cases of posterior gap (fi rst day) and 1 case of posterior retraction (fi rst month). The HOAs had not signifi cantly changed preoperatively and after 1 month. CONCLUSIONS: The femtosecond laser refractive lens surgery incision is stable and does not signifi cantly change the HOA.

إن التثبيت الداخلي المحدود لكسور عظمة حق مفصل الحوض قد أصبح طريقة علاجية للكسور الغير منقولة مع تثبيت الكسور باستخدام مسامير من خلال فتحات صغيرة الجلد. هذه الدراسة تصف طريقة علاجية مستحدثة جديدة لكسور عظمة حق مفصل الحوض باستخدام الشق المحدود جار العضلة المستقيمة بجدار البطن لتصليح الكسور المنقولة وتثبيتها داخلياً باستخدام مسامير من خلال فتحات صغيرة الجلد.

إن التثبيت الداخلي المحدود لكسور عظمة حق مفصل الحوض قد أصبح طريقة علاجية للكسور الغير منقولة مع تثبيت الكسور باستخدام مسامير من خلال فتحات صغيرة الجلد. هذه الدراسة تصف طريقة علاجية مستحدثة جديدة لكسور عظمة حق مفصل الحوض باستخدام الشق المحدود جار العضلة المستقيمة بجدار البطن لتصليح الكسور المنقولة وتثبيتها داخلياً باستخدام مسامير من خلال فتحات صغيرة الجلد.

إن التثبيت الداخلي المحدود لكسور عظمة حق مفصل الحوض قد أصبح طريقة علاجية للكسور الغير منقولة مع تثبيت الكسور باستخدام مسامير من خلال فتحات صغيرة الجلد. هذه الدراسة تصف طريقة علاجية مستحدثة جديدة لكسور عظمة حق مفصل الحوض باستخدام الشق المحدود جار العضلة المستقيمة بجدار البطن لتصليح الكسور المنقولة وتثبيتها داخلياً باستخدام مسامير من خلال فتحات صغيرة الجلد.

إن التثبيت الداخلي المحدود لكسور عظمة حق مفصل الحوض قد أصبح طريقة علاجية للكسور الغير منقولة مع تثبيت الكسور باستخدام مسامير من خلال فتحات صغيرة الجلد. هذه الدراسة تصف طريقة علاجية مستحدثة جديدة لكسور عظمة حق مفصل الحوض باستخدام الشق المحدود جار العضلة المستقيمة بجدار البطن لتصليح الكسور المنقولة وتثبيتها داخلياً باستخدام مسامير من خلال فتحات صغيرة الجلد.

Many non-fusion instrumentation techniques were shown to be effective in controlling the progression of spinal deformity in young children, and maintaining the growth potential of the immature spine. Of these, the Vertical Expandable Prosthetic Titanium Rib (VEPTR) has been approved by the FDA in the treatment of Thoracic Insufficincy Syndrome (TIS) in skeletally immature patients. The aim of this presentation is to demonstrate the effect of expansion thoracoplasty and implantation of a VEPTR in three children with progressive spinal deformity despite previous convex- sided hemiepiphysiodesis. Three patients with progressive congenital thoracic scoliosis, who had previously undergone anterior and posterior convex hemiepiphysiodesis of the thoracic spine at the age of 17 to 36 months, were treated with expansion thoracoplasty of the concavity using the VEPTR device at the age of 5 to 9 years. They were evaluated after a minimum of two years in order to assess the effect of the procedure on the spinal deformity, spinal growth, and chest wall expansion. At the latest follow-up, the Cobb angle of the fused segments, the length of the thoracic spine from T1 o T12, and the Space Availabel for the Lungs (SAL) improved in all three patients. Detailed results are listed in the next table. Thoracic spinal height increased from 11.25 to 15.68 cm in patient 1, from 15.40 to 17.15 cm in patient 2, and from 16.35 to 18.25 cm in patient 3. Sagittal Balance improved from +4.5 cm to neurtral in patient 1, and from +6 cm to +1 cm in patient 2, but deteriorated from -0.5 cm to -1.5 cm in patient 3. Expansion Thoracoplasty and VEPTR implantation seem to be effective in rigid congenital scoliosis even after prior convex-sided hemipepiphysiodesis.