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Improvement of post-harvest quality of Balady lime fruit with Aloe vera gel and tea tree oil against green mold disease caused by Penicillium digitatum

Research Abstract

Penicillium digitatum Sacc is a common postharvest pathogen affecting lime fruits. It causes significant losses in postharvest lime production. An emerging alternative to chemical control is the use of environmentally friendly such as edible and biodegradable films and packaging. The goal of the current study was to test different concentrations of Aloe vera gel (5, 15, 25, 50, 75, and 100% v/v), tea tree oil coating (0.5, 1, 2.5, 5, and 10% v/v), and their combination for controlling P. digitatum in vitro. The results revealed that specific concentrations of Aloe vera gel (75% and 100% v/v), tea tree oil (2.5, 5, and 10% v/v), and Aloe vera gel (25%) + tea tree oil (1%) v/v showed the highest inhibitory effect against the pathogen. The most effective concentrations of Aloe vera gel (75%), tea tree oil (2.5%), and Aloe vera gel (25%) + tea tree oil (1%) were evaluated against the green mold disease of lime under natural infection through storage period (0, 7, 14, 21, and 28 days). Compared to the control group, all edible layers demonstrated a significant delay and reduced in disease severity percentage throughout the storage periods. However, coating lime fruits with a combination of Aloe vera gel (25%) + tea tree oil (1%) successfully prevented the green mold disease of lime fruits over 28 days. The impact of these coatings was evaluated on lime fruit quality maintenance (weight loss, firmness, juice weight, total soluble solids, total acidity, pH, vitamin C, lipid peroxidation content, total phenols, total flavonoid, and total protein) over 28 days of storage during the 2021 and 2022 seasons. All treatments improved fruit quality through the storage period compared with untreated fruits. Treated lime fruits with Aloe vera gel (25%) + tea tree oil (1%) exhibited slight reductions in weight loss and juice weight compared to alternative treatments as an average of the two successive seasons. Simultaneously, this treatment improved fruit firmness, vitamin C levels, total phenols, total flavonoid, and total protein when contrasted with other treatments as an average of the two studied seasons. These findings show that the combination of Aloe vera gel and tea tree oil coatings enhances the shelf life and maintains the quality of lime fruits. They can also be used as an environmentally acceptable, chemical-free alternative treatment to preserve the postharvest quality of lime fruit.

Research Authors
Eman A. A. Abou-Zaid, Azza S. Hussein, Raoof Sultan, Kamal A. M. Abo-Elyousr, Nashwa M. A. Sallam, Hadeel M. M. Khalil Bagy
Research Date
Research Department
Research Journal
Journal of Plant Pathology
Research Member
Research Pages
1715–1729
Research Publisher
Springer International Publishing
Research Vol
106
Research Website
https://link.springer.com/article/10.1007/s42161-024-01705-0
Research Year
2024

Exogenous Pre-harvest Application of Abscisic and Jasmonic Acids Improves Fruit Quality by Enhancing Sugar Synthesis and Reducing Acidity in Pomegranate (Punica granatum L. cv. Wonderful)

Research Abstract

One of the major issues with pomegranate production in arid and semi-arid climate is the white arils of fruit. The known plant regulators abscisic and jasmonic acids play a variety of roles in plant growth and the reduction of abiotic stresses. However, little is known about their application to improve the quality of pomegranate fruit, particularly white arils, in semi-arid environments. Herein, the experiments, in this study, were performed in two consecutive seasons 2020 and 2021 on 10-year-old Wonderful pomegranate trees to spotlight the improvement of the aril coloration and fruit quality by spraying with abscisic and jasmonic acids under semi-arid climatic conditions. Fifteen Wonderful pomegranate trees were subjected to five treatments (three replications for each). The tested treatments included abscisic acid (ABA) at the concentrations of 600 and 800 µg−1 mL, jasmonic acid (JA) at the concentrations of 10 and 15 Mm, and the control treatment (distilled water). The physical and chemical characteristics of the fruit quality of Wonderful pomegranate improved significantly when the trees were treated with abscisic or jasmonic acids. The foliar addition of ABA at 600 and 800 µg−1 mL increased the anthocyanin index by 96.74 and 114.75%, respectively, in the first year and by 49.48 and 67.62% in the second year, in comparison with the control. The foliar addition of ABA at 800 µg−1 mL was more effective than jasmonic acid in enhancing most of the chemical fruit properties, especially the anthocyanin index. The high level of ABA (800 µg−1 mL) gave the minimum fruit acidity and the highest fruit quality. Abscisic acid induces the fruit acidity and enhances the aril coloration in Wonderful pomegranate. In semi-arid conditions, it is recommended to spray the Wonderful pomegranate trees with abscisic acid at 800 µg−1 mL to improve the fruit quality and to increase the aril coloration.

Research Authors
Azza S. Hussein, Rashad A. Ibrahim, Mamdouh A. Eissa
Research Date
Research Department
Research Journal
Journal of Soil Science and Plant Nutrition
Research Pages
2237–2246
Research Publisher
Springer International Publishing
Research Vol
23
Research Website
https://link.springer.com/article/10.1007/s42729-023-01175-w
Research Year
2023

Response of Some Bread Wheat Cultivars to Foliar Applications Time of Salicylic Acid Concentrations Under the New Valley Conditions

Research Abstract

A field experiment was carried out during 2021/2022 and 2022/2023 seasons in Almawhoub, Aldakhla, New valley Governorate, Egypt. This investigation aimed to study the effect of spraying three wheat cultivars with different concentrations of salicylic acid (SA) on the growth and yield traits. Randomized complete block design (RCBD) using split plots in strips arrangement with three replications was used.
The cultivars Sakha 95, Giza 171, and Gemmiza 11 were assigned in vertical strips while spraying with three concentrations of salicylic acid (0, 45, 60, and 75 ppm) were allocated in horizontal strips. In addition, three SA application times, i.e., 30, 45 and 60 days after planting were allocated in the sub- units. The experimental unit area was 10.50 m2.
The results proved that mean effect of spraying wheat plants by salicylic acid at 75 ppm in both seasons gave the highest mean values of  plant height (116.5 and 121.8 cm), maximum number of spike/ m2 (344.4 and 358.2 spike / m2), the highest 1000 grain weight (49.5 and 51.66 g), and the maximum grain yield (19.37 and 20.18 ardab/ fed)in the first and second seasons respectively. Foliar application at 60 days from planting registered the maximum average values of all studied traits under investigation. Spike length was insignificantly affected. Here too, the obtained results showed that Sakha 95 cultivar surpassed the other studied cultivars in grain yield (ardab/ fed), No. of spike/m2, and 1000 grain/weight. Gemmiza 11 was better than the other cultivars in plant height and spike length. Most of interactions revealed that spraying wheat plants by 75 ppm salicylic acid after 60 days from planting registered the highest values of studied traits.

Research Authors
Mohamed A. E. Mohamed; Elmahdy A. Teama; El-Saady A. Ali; Mohamed T. Said, and Howida E. Abdelkader
Research Date
Research Department
Research File
Research Journal
Assiut Journal of Agricultural Sciences
Research Pages
60-77
Research Vol
56
Research Website
https://ajas.journals.ekb.eg/article_410012.html?lang=en
Research Year
2025

Impact of Gibberellic Acid and Phosphorus Application Time on Yield and Its Attributes for Some Faba Bean Cultivars

Research Abstract

A field experiment was carried out at the Experimental Farm of Agronomy Dept., Assiut University, during the 2021/2022 and 2022/2023 seasons. The work aimed to investigate the effect of three phosphate fertilizer applied dates (before the first P1, second P2, and third irrigation P3) and four gibberellic acid levels (0, 50 G1, 150 G2, and 250 G3 ppm) on three fab bean cultivars (Misr1, Sakha 4 and Giza 843). The experiment was laid out in randomized complete block design (RCBD) using a strip plot within a split plot with three replicates. The plot area was 9 m2.
The results revealed that maximum seed yield in addition to yield attributes were produced from plants receiving 250 or 150ppm gibberellic acid with third and second phosphorus application in both seasons. Giza 843 cultivar was significantly higher seed index, biological and seed yield in both seasons. The maximum Pod number/plant and seeds number/pod in the 1st season were recorded by 250ppm gibberellic acid concentration under the first and second phosphorus application dates (G3×Pand G3×P 2) in the 2nd season. Moreover, the maximum seeds and biological yield were recorded by 250ppm gibberellic acid concentration under the third phosphorus application date (G3×P3) in both seasons. Furthermore, the tallest plants, maximum seed index and biological yield were recorded by Giza 843 cultivar under the second and third phosphorus application dates (V3×Pand V3×P3) in both seasons. Maximum pod number/plant and seed yield were recorded by Misr1 cultivar under the first and third phosphorus application dates (V1×Pand V1×P3) in both seasons.

Research Authors
Warda Hamdy Sayed, Kamel Ali Abdel-Rahamn Othman,Ragab Ahmed El-Sayed Dawood,Inaam Helmy Galal Mohamed; Mohamed Tharwat Said Abd-El-all
Research Date
Research Department
Research File
Research Journal
Assiut Journal of Agricultural Sciences
Research Pages
48-59
Research Vol
56
Research Website
https://ajas.journals.ekb.eg/article_407883.html
Research Year
2024

Comprehensive characterisation of taste and aroma profiles of Daokou red-cooked chicken by GC-IMS and GC–MS combined with chemometrics

Research Abstract

Taste and aroma profiles of Daokou red-cooked chickens (DRCs) were analysed using different techniques and methods. Multivariate data analysis revealed that e-nose and e-tongue could successfully discriminate DRCs of various sources based on their taste and aroma profiles. Flavour nucleotide content and umami concentration of breasts were higher than those of thighs. Additionally, a fingerprint was established to analyse the distribution of different volatile compounds using gas chromatography-ion mobility spectrometry. A total number of 54 volatile components was identified in the DRCs, mostly aldehydes, esters, ketones, terpenes and alcohols. Odour activity value demonstrated that nine volatile components, including 1-octen-3-ol, 2-pentylfuran, eucalyptol, nonanal, estragole, anisaldehyde, anethole, eugenol and hexanal, could be considered as the characteristic flavour compounds of DRCs. Furthermore, partial least squares-discriminant analysis (PLS-DA) analysis showed that sweet tastes, anethole, 5′-inosine monophosphate, eugenol, eucalyptol, umami tastes, sour tastes, bitter tastes, 2,3-butanedione, hexanal, hypoxanthine, inosine and d-limonene were significant factors that caused the differences among DRCs of different sources. Sensory analysis revealed variations in consumer preferences of DRCs from different suppliers. Obtained results will help in understanding of how taste and flavour compounds affect quality and preferences of DRCs, which will help manufacturers improve processing methods and promote consumption of DRCs.

Research Authors
Xiangxiang Sun, Yumei Yu, Ahmed S. M. Saleh, Xinyu Yang, Jiale Ma, Dequan Zhang, Wenhao Li, Zhenyu Wang
Research Date
Research Journal
International Journal of Food Science and Technology
Research Member
Research Pages
4288-4300
Research Publisher
Wiley
Research Vol
58
Research Website
https://doi.org/10.1111/ijfs.16528
Research Year
2023

Insights into the interactions between etheric compounds and myofibrillar proteins using multi-spectroscopy, molecular docking, and molecular dynamics simulation

Research Abstract

This study aimed to examine how the addition of etheric compounds (EC) affects the characteristics of myofibrillar proteins (MP) and to understand underlying interaction mechanisms. Fourier transform infrared spectroscopy confirmed that the EC-MP complex was formed through hydrogen bonding. The addition of EC resulted in an increase in the α-helix content and a decrease in the β-sheet content of MP, which would promote the protein unfolding. The unfolding of MP led to aggregation and formation of larger and non-uniform particles. As a result, the exposure of negative charge on the MP surface was enhanced, and zeta potential was decreased from −5.33 mV to −7.45 mV. Moreover, the EC-induced modification of MP conformation resulted in a less rigid three-dimensional network structure of MP gel and enhanced the discharge of aldehyde compounds (C > 6). Moreover, the rheological characteristics of MP were enhanced by the suppression of protein–protein interactions due to the MP unfolding. Molecular dynamics simulations revealed that anethole reduced the binding capacity of myosin to decanal by raising its binding energy from –22.22 kcal/mol to −19.38 kcal/mol. In the meantime, anethole competed for the amino acid residue (PHE165) where myosin connects to decanal. This caused the hydrogen bonds and hydrophobic contacts between the two molecules to dissolve, altering myosin's conformation and releasing decanal. The results might be useful in predicting and controlling the ability of proteins to release and hold onto flavors.

Research Authors
Xiangxiang Sun a b c , Ahmed S.M. Saleh d , Zhenyu Wang b c , Yumei Yu b c , Wenhao Li a , Dequan Zhang b c
Research Date
Research Journal
Food Research International
Research Member
Research Pages
1-11
Research Publisher
Elsevier
Research Vol
175
Research Website
https://doi.org/10.1016/j.foodres.2023.113787
Research Year
2024

Curcumin-loaded soybean-dextran conjugate nanogels: Construction, characterization, and incorporation into orange juice beverage

Research Abstract

Although the health benefits of lipophilic bioactive compounds have received tremendous attention from both researchers and manufacturers, the practical application of these micronutrients in aqueous foods is still challenging due to their water insolubility and environmental instability. Therefore, curcumin (Cur) was selected to be encapsulated in soybean protein isolate-dextran conjugate-based nanogels (SDCNG) via the Maillard reaction combined with protein self-assembly to be incorporated into orange juice beverage (OJB) in this study. Cur-loaded SDCNG (Cur-SDCNG) prepared by adding Cur into the SDCNG solution exhibited an encapsulation efficiency of 89.10% ± 1.40% and a loading capacity of 17.11% ± 0.27%, enhanced 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical (•OH)-scavenging activities and ferrous reducing power, increased retention rate at both natural light and dark environment, and strengthened storage stability. Moreover, the loaded Cur showed a greater bioaccessibility (55.41% ± 0.71%) than free Cur (17.41% ± 1.04%). The OJB fortified with Cur by adding Cur-SDCNG exhibited an increasing color difference, particle size, polydispersity index (PDI), protein content, total soluble solid (TSS), and sensory score with the increasing Cur concentration. DPPH radical and •OH-scavenging activities and ferrous reducing power also further enhanced. The Cur-fortified OJB with an orange juice concentration of 30% exhibited improved quality attributes and satisfactory stability reflected by the increased TSS and sensory score, acceptable particle size, PDI, centrifugal sedimentation rate, and Cur retention rate under cold conditions. In conclusion, the findings confirmed the effectiveness of Cur encapsulation in SDCNG and the application feasibility of Cur-SDCNG in acidic beverages simultaneously fortified with lipophilic bioactive compounds and vegetable proteins.

Research Authors
Yixi Sun a , Wenting Yue a , Xianrong Xiang a , Zhihan Chen a , Junpeng Chen a , Shasha Li a , Shuxiang Liu a , Ahmed S.M. Saleh b , Wen Qin a , Qing Zhang a
Research Date
Research Journal
Food Bioscience
Research Member
Research Pages
1-12
Research Publisher
Elsevier
Research Vol
59
Research Website
https://doi.org/10.1016/j.fbio.2024.104140
Research Year
2024

Insights into flavor formation of braised chicken: Based on E-nose, GC–MS, GC-IMS, and UPLC-Q-Exactive-MS/MS

Research Abstract

Effects of braising duration on volatile organic compounds (VOCs) and lipids in chicken were investigated. Aroma profiles identified by an electronic nose were effective in differentiating braising stages. During braising process, a total of 25 key VOCs were detected in braised chicken, and sample braised for 210 min exhibited the highest level of key VOCs. Additionally, a gas chromatography mass spectrometry fingerprint was established to evaluate the distribution of VOCs throughout the braising process. Partial least square discriminant analysis indicated that 2-heptanone, 3-methyl-2-butanone, octanal, nonanal, butanal, (E)-2-pentenal, 1-octen-3-ol, 1-hexanol, pentanal, hexanal, and 1-pentanol significantly affected flavor characteristics of braised chicken. Furthermore, 88 differential lipids were screened, and glycerolipids metabolic was found to be main metabolic pathway during braising process. Triglycerides (TG) and phosphatidyl ethanolamine (PE), such as TG (16:0/18:1/18:2), TG (18:0/18:1/18:2), TG (18:1/18:2/18:3), TG (18:1/18:1/18:2), PE (O-18:2/18:2), PE(O-18:2/18:1), and TG (16:0/16:1/18:2), played a vital role in the generation of VOCs.

Research Authors
Xiangxiang Sun a b c , Yumei Yu a b , Zhenyu Wang a b , Kumayl Hassan Akhtar a b , Ahmed S.M. Saleh d , Wenhao Li c , Dequan Zhang a b
Research Date
Research Journal
Food Chemistry
Research Member
Research Pages
1-10
Research Publisher
Elsevier
Research Vol
448
Research Website
https://doi.org/10.1016/j.foodchem.2024.138972
Research Year
2024

Conformational changes induced by selected flavor compounds from spices regulate the binding ability of myofibrillar proteins to aldehyde compounds

Research Abstract

Interactions among flavor compounds from spices (FCS) and myofibrillar proteins (MP) were investigated. Fluorescence and Fourier transform infrared spectroscopy showed that hydrogen bonding and hydrophobic interactions were the main binding forces between FCS and MP. The FCS increased the particle size and SH content of MP and caused a reduction of zeta potential from −5.23 to −6.50 mV. Furthermore, FCS could modify the binding ability of MP and aldehydes. Eugenol reduced the ability of MP to bond with aldehydes by 22.70–47.87 %. Molecular dynamics simulations demonstrated that eugenol may combat nonanal to attain binding site of amino acid residue (PHE165) and induce protein conformational changes. Electrostatic interactions and van der Waals forces within myosin-nonanal may be disrupted by these alterations, which could reduce stability of complex and cause release of nonanal. This study could provide new insights into regulating the ability of proteins to release and hold flavors.

Research Authors
Xiangxiang Sun a b c , Yumei Yu a b , Ahmed S.M. Saleh d , Kumayl Hassan Akhtar a b , Wenhao Li c , Dequan Zhang a b , Zhenyu Wang a b
Research Date
Research Journal
Food Chemistry
Research Member
Research Pages
1-10
Research Publisher
Elsevier
Research Vol
451
Research Website
https://doi.org/10.1016/j.foodchem.2024.139455
Research Year
2024

Effect of β-sitosterol and palmitic acid mass ratio on structural, physicochemical, and rheological properties of rice bran oil-based oleogel

Research Abstract

Edible oil-based oleogels provide a viable and healthier alternative to traditional saturated fats. This study aimed to develop oleogels using rice bran oil combined with β-Sitosterol (Sit) and Palmitic Acid (PA). Structural, physicochemical, and rheological properties of the prepared oleogels were investigated. The results showed that Sit alone was insufficient to create a stable oleogel. However, when Sit was combined with PA, a firm and stable oleogel was obtained. The gel network was formed and stabilized through physical non-covalent interactions such as hydrogen bonding. Additionally, x-ray diffraction analysis indicated that the oleogel molecules rearranged and underwent a homogeneous polycrystalline transition due to the different gelator combinations, revealing the coexistence of β and β′ crystal types in the oleogels. Moreover, the firmness and oil binding capacity of the oleogels increased with higher PA content in the gelator mixture. Furthermore, the storage modulus (G′) and loss modulus (G″) were frequency-independent, with G′ significantly higher than G". The results suggest that fat-like oleogel can be produced using a combination of Sit and PA at mass ratios of Sit2:PA3 or Sit1:PA4 (w/w).

Research Authors
Shu Yang a , Xueping Zhang d , Ahmed S.M. Saleh c , Lishuang Wang b , Yumin Duan b , Zhigang Xiao b
Research Date
Research Journal
LWT-Food Science and Technology
Research Member
Research Pages
1-10
Research Publisher
Elsevier
Research Vol
209
Research Website
https://doi.org/10.1016/j.lwt.2024.116775
Research Year
2024
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