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Congratulations - The Faculty volleyball team won third place at the Assiut University level

تحت رعاية السيدة الأستاذ الدكتور / مديحه درويش عميد الكلية والمشرف العام علي الانشطه الطلابيه بالجامعة
والسيد الاستاذ الدكتور / مؤمن عبد العظيم وكيل الكلية لشئون التعليم والطلاب 
فوز فريق الكلية للكرة الطائرة بالمركز الثالث علي مستوي جامعة أسيوط بعد فوزه علي كلية الصيدليه

مشرف الفرق ا. محمد حسن

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An integrated calibration strategy for the development and validation of an LC-MS/MS method for accurate quantification of egg allergens (Gal d 1–6) in foods

Research Abstract

Accurate determination of egg allergens in food is vital for allergen management and labeling. However, quantifying egg allergens with mass spectrometry poses challenges and lacks validation methods. Here, we developed and validated an LC-MS/MS method for quantifying egg allergens (Gal d 1–6) in foods. Sample extraction, enzymatic digestion, purification, proteins/peptides selection, and calibration curves were optimized. VMVLC[+57]NR (Gal d 1) and GTDVQAWIR (Gal d 5) exhibited outstanding sensitivity and stability, serving as quantitation markers for egg white and yolk. Using a matrix-matched calibration curve with allergen ingredients as calibrants and labeled peptides as standards, we achieved highly accurate quantitation. Validation involved spiking egg protein into egg-free foods, showing excellent sensitivity (LOQ: 1–5 mg/kg), accuracy (62.4 %–88.5 %), and reproducibility (intra-/inter-day precision: 3.5 %–14.2 %/8.2 %–14.6 %). Additionally, we successfully applied this method to commercial food analysis. These findings demonstrate optimal allergen selection, peptides, and calibration strategy are crucial parameters for food allergen quantification via MS-based methods.

Research Authors
Shupeng Yang , Jingjing Chen, Mohamed F. Abdallah, Haopeng Lin, Peijie Yang, Jianxun Li, Rong Zhang, Qianqian Li, Peng Lu, Suyan Liu, Yi Li
Research Date
Research Department
Research File
Research Journal
Food Chemistry
Research Member
Research Pages
137922
Research Publisher
Elsevier
Research Rank
Q1
Research Website
https://www.sciencedirect.com/science/article/pii/S0308814623025402
Research Year
2023

Immunopathogenesis of the Canadian Delmarva (DMV/1639) infectious bronchitis virus (IBV): Impact on the reproductive tract in layers

Research Authors
Mohamed SH Hassan, Shahnas M Najimudeen, Ahmed Ali, Danah Altakrouni, Dayna Goldsmith, Carla S Coffin, Susan C Cork, Frank van der Meer, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Microbial Pathogenesis
Research Pages
105513
Research Publisher
Academic Press
Research Vol
166
Research Website
https://sciencedirect.com/science/article/pii/S0882401022001267
Research Year
2022

Replication of infectious bronchitis virus (IBV) Delmarva (DMV)/1639 variant in primary and secondary lymphoid organs leads to immunosuppression in chickens

Research Authors
Shahnas M Najimudeen, Reham M Abd-Elsalam, Hiruni A Ranaweera, Ishara M Isham, Mohamed SH Hassan, Muhammad Farooq, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Virology
Research Pages
109852
Research Publisher
Academic Press
Research Vol
587
Research Website
https://www.sciencedirect.com/science/article/pii/S0042682223001654
Research Year
2023

Impact of Maternal Antibodies on Infectious Bronchitis Virus (IBV) Infection in Primary and Secondary Lymphoid Organs of Chickens

Research Authors
Ishara M Isham, Mohamed SH Hassan, Reham M Abd-Elsalam, Hiruni A Ranaweera, Motamed E Mahmoud, Shahnas M Najimudeen, Awais Ghaffar, Susan C Cork, Ashish Gupta, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Vaccines
Research Pages
1216
Research Publisher
MDPI
Research Vol
11 (7)
Research Website
https://www.mdpi.com/2076-393X/11/7/1216
Research Year
2023

The impact of the experimental route of challenge on the host responses and pathogenesis of the Canadian Delmarva (DMV/1639) infectious bronchitis virus infection in laying chickens

Research Authors
Mohamed SH Hassan, Reham M Abd-Elsalam, Natalya Ratcliff, Heshanthi Herath-Mudiyanselage, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Veterinary Immunology and Immunopathology
Research Pages
110623
Research Publisher
Elsevier
Research Vol
261
Research Website
https://www.sciencedirect.com/science/article/abs/pii/S0165242723000776
Research Year
2023

Efficacy of Two Vaccination Strategies against Infectious Bronchitis in Laying Hens

Research Authors
Ahmed Ali, Mohamed SH Hassan, Shahnas M Najimudeen, Muhammad Farooq, Salama Shany, Mounir Mohamed El-Safty, Adel A Shalaby, Mohamed Faizal Abdul-Careem
Research Date
Research Journal
Vaccines
Research Pages
338
Research Publisher
MDPI
Research Vol
11 (2)
Research Website
https://www.mdpi.com/2076-393X/11/2/338
Research Year
2023
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