The present study was conducted on the period from April 2008 to July 2009 and included at 150 cattle and 35 Egyptian buffalo. The age of these animals ranged from one day to above five years old. The animals belonged to farms and villages of EL-Wady EL-geded, Assiut, ELFayoum, EL- Minia and Sohage Governorates. The results of the present study cleared that the (Tams-1 primer) based PCR assay was the most sensitive test in detection of the infection with tropical theileriosis in all cases (acute, chronic and carriers). The infection rates in blood and lymph samples taken from cattle were 65.6% and 45.3%, respectively. On the other hand the infection rates were 16.7% and 25% in blood and lymph samples taken from buffaloes, respectively. PCR used as golden standard test to evaluate the conventional tests. The sensitivity of this method was 58.3% and 50% in cattle and buffaloes, respectively. While the specificity were 100% in both cattle and buffaloes. We concluded that, Tams-1 target–based PCR is the most sensitive and specific test used for diagnosis of the disease in either acute or chronic cases and also in carrier animals of tropical theileriosis.

The present study was conducted on the period from April 2008 to July 2009 and included at 150 cattle and 35 Egyptian buffalo. The age of these animals ranged from one day to above five years old. The animals belonged to farms and villages of EL-Wady EL-geded, Assiut, ELFayoum, EL- Minia and Sohage Governorates. The results of the present study cleared that the (Tams-1 primer) based PCR assay was the most sensitive test in detection of the infection with tropical theileriosis in all cases (acute, chronic and carriers). The infection rates in blood and lymph samples taken from cattle were 65.6% and 45.3%, respectively. On the other hand the infection rates were 16.7% and 25% in blood and lymph samples taken from buffaloes, respectively. PCR used as golden standard test to evaluate the conventional tests. The sensitivity of this method was 58.3% and 50% in cattle and buffaloes, respectively. While the specificity were 100% in both cattle and buffaloes. We concluded that, Tams-1 target–based PCR is the most sensitive and specific test used for diagnosis of the disease in either acute or chronic cases and also in carrier animals of tropical theileriosis.

The present study was conducted on the period from April 2008 to July 2009 and included at 150 cattle and 35 Egyptian buffalo. The age of these animals ranged from one day to above five years old. The animals belonged to farms and villages of EL-Wady EL-geded, Assiut, ELFayoum, EL- Minia and Sohage Governorates. The results of the present study cleared that the (Tams-1 primer) based PCR assay was the most sensitive test in detection of the infection with tropical theileriosis in all cases (acute, chronic and carriers). The infection rates in blood and lymph samples taken from cattle were 65.6% and 45.3%, respectively. On the other hand the infection rates were 16.7% and 25% in blood and lymph samples taken from buffaloes, respectively. PCR used as golden standard test to evaluate the conventional tests. The sensitivity of this method was 58.3% and 50% in cattle and buffaloes, respectively. While the specificity were 100% in both cattle and buffaloes. We concluded that, Tams-1 target–based PCR is the most sensitive and specific test used for diagnosis of the disease in either acute or chronic cases and also in carrier animals of tropical theileriosis.

The present study was conducted on the period from April 2008 to July 2009 and included at 150 cattle and 35 Egyptian buffalo. The age of these animals ranged from one day to above five years old. The animals belonged to farms and villages of EL-Wady EL-geded, Assiut, ELFayoum, EL- Minia and Sohage Governorates. The results of the present study cleared that the (Tams-1 primer) based PCR assay was the most sensitive test in detection of the infection with tropical theileriosis in all cases (acute, chronic and carriers). The infection rates in blood and lymph samples taken from cattle were 65.6% and 45.3%, respectively. On the other hand the infection rates were 16.7% and 25% in blood and lymph samples taken from buffaloes, respectively. PCR used as golden standard test to evaluate the conventional tests. The sensitivity of this method was 58.3% and 50% in cattle and buffaloes, respectively. While the specificity were 100% in both cattle and buffaloes. We concluded that, Tams-1 target–based PCR is the most sensitive and specific test used for diagnosis of the disease in either acute or chronic cases and also in carrier animals of tropical theileriosis.

The prevalence of parasitic infestation was studied in camels. A total of 460 camels of different age, sex and localities were examined. Collected fecal samples were subjected to sedimentation and floatation techniques and then examined for parasitic eggs. Fecal examination revealed that 26.9% (124 out of 460) of the camels parasitic eggs in their feces. Different types of parasites eggs prevalence were Strongylus sp. 8.2%, Trichostrongylus sp. 6.7, Trichuris sp. 4.7%, and mixed infections with parasites were recorded in 7.1 %. In these results recorded hard ticks infestation in camels 28.6%. Sarcoptic scabei var cameli (mange) infest camels 12.1%. Moreover, camels are infected by Trypanosoma evansi 9.5%. The prevalence of parasitic infestation internal and external observed in the present study suggests that parasites are more common in the farms or herds that examined and may be leads to economic camel production losses, there is need to control program application.