The current experiment was designed to evaluate the effects of dietary supplementations of zinc oxide nanoparticles (ZONPs) on some behavioural, performance, welfare and histopathological changes in broilers exposed to multidrug resistant Staphylococcus aureus (MRSA)-induced footpad dermatitis(FPD). Eighty-four males Indian River (IR) broilers were randomly allotted to six different dietary treatments as follows C-ve, C+ve, 10, 20, 30 and 40 ppm ZONPs from 7- 49ds of age. At day 28, broilers (n = 70) were subcutaneously injected 0.5mL of saline containing 5.3 × 107 cfu/ mL of S. aureus (MRSA) in each metatarsal foot pad. Control (non-infected) broilers were given 0.5 mL of saline (n = 14). Results clarified that non-infected birds and ZONPs fed birds had significantly higher standing and feeding activities and lower resting activities in comparison with the infected group. Also, the S. aureus infected group had significantly lower body weight gain (BWG) and higher feed conversion ratio (FCR) than the noninfected group. In addition, the non-infected birds and ZONPs groups had significantly lower object crossing and tonic immobility times (TI) and gait scores (GS) in comparison with the S. aureus group. Only ZONPs 30, 40 ppm and non-infected groups had significantly higher latency to lie time (LLT) and lower serum cortisol level in comparison with the S. aureus group. Moreover, 4 there were significant changes in the gross lesion score and histopathological lesion between the different groups. In conclusion, the dietary supplementation of ZONPs can reduce S. aureus induced negative effects of FPD in broilers

The current experiment was designed to evaluate the effects of dietary supplementations of zinc oxide nanoparticles (ZONPs) on some behavioural, performance, welfare and histopathological changes in broilers exposed to multidrug resistant Staphylococcus aureus (MRSA)-induced footpad dermatitis(FPD). Eighty-four males Indian River (IR) broilers were randomly allotted to six different dietary treatments as follows C-ve, C+ve, 10, 20, 30 and 40 ppm ZONPs from 7- 49ds of age. At day 28, broilers (n = 70) were subcutaneously injected 0.5mL of saline containing 5.3 × 107 cfu/ mL of S. aureus (MRSA) in each metatarsal foot pad. Control (non-infected) broilers were given 0.5 mL of saline (n = 14). Results clarified that non-infected birds and ZONPs fed birds had significantly higher standing and feeding activities and lower resting activities in comparison with the infected group. Also, the S. aureus infected group had significantly lower body weight gain (BWG) and higher feed conversion ratio (FCR) than the noninfected group. In addition, the non-infected birds and ZONPs groups had significantly lower object crossing and tonic immobility times (TI) and gait scores (GS) in comparison with the S. aureus group. Only ZONPs 30, 40 ppm and non-infected groups had significantly higher latency to lie time (LLT) and lower serum cortisol level in comparison with the S. aureus group. Moreover, 4 there were significant changes in the gross lesion score and histopathological lesion between the different groups. In conclusion, the dietary supplementation of ZONPs can reduce S. aureus induced negative effects of FPD in broilers

The current experiment was designed to evaluate the effects of dietary supplementations of zinc oxide nanoparticles (ZONPs) on some behavioural, performance, welfare and histopathological changes in broilers exposed to multidrug resistant Staphylococcus aureus (MRSA)-induced footpad dermatitis(FPD). Eighty-four males Indian River (IR) broilers were randomly allotted to six different dietary treatments as follows C-ve, C+ve, 10, 20, 30 and 40 ppm ZONPs from 7- 49ds of age. At day 28, broilers (n = 70) were subcutaneously injected 0.5mL of saline containing 5.3 × 107 cfu/ mL of S. aureus (MRSA) in each metatarsal foot pad. Control (non-infected) broilers were given 0.5 mL of saline (n = 14). Results clarified that non-infected birds and ZONPs fed birds had significantly higher standing and feeding activities and lower resting activities in comparison with the infected group. Also, the S. aureus infected group had significantly lower body weight gain (BWG) and higher feed conversion ratio (FCR) than the noninfected group. In addition, the non-infected birds and ZONPs groups had significantly lower object crossing and tonic immobility times (TI) and gait scores (GS) in comparison with the S. aureus group. Only ZONPs 30, 40 ppm and non-infected groups had significantly higher latency to lie time (LLT) and lower serum cortisol level in comparison with the S. aureus group. Moreover, 4 there were significant changes in the gross lesion score and histopathological lesion between the different groups. In conclusion, the dietary supplementation of ZONPs can reduce S. aureus induced negative effects of FPD in broilers

The current experiment was designed to evaluate the effects of dietary supplementations of zinc oxide nanoparticles (ZONPs) on some behavioural, performance, welfare and histopathological changes in broilers exposed to multidrug resistant Staphylococcus aureus (MRSA)-induced footpad dermatitis(FPD). Eighty-four males Indian River (IR) broilers were randomly allotted to six different dietary treatments as follows C-ve, C+ve, 10, 20, 30 and 40 ppm ZONPs from 7- 49ds of age. At day 28, broilers (n = 70) were subcutaneously injected 0.5mL of saline containing 5.3 × 107 cfu/ mL of S. aureus (MRSA) in each metatarsal foot pad. Control (non-infected) broilers were given 0.5 mL of saline (n = 14). Results clarified that non-infected birds and ZONPs fed birds had significantly higher standing and feeding activities and lower resting activities in comparison with the infected group. Also, the S. aureus infected group had significantly lower body weight gain (BWG) and higher feed conversion ratio (FCR) than the noninfected group. In addition, the non-infected birds and ZONPs groups had significantly lower object crossing and tonic immobility times (TI) and gait scores (GS) in comparison with the S. aureus group. Only ZONPs 30, 40 ppm and non-infected groups had significantly higher latency to lie time (LLT) and lower serum cortisol level in comparison with the S. aureus group. Moreover, 4 there were significant changes in the gross lesion score and histopathological lesion between the different groups. In conclusion, the dietary supplementation of ZONPs can reduce S. aureus induced negative effects of FPD in broilers

This study was conducted in the kingdom of Saudi Arabia during the period from (August 2019 to August 2020) to investigate the sarcoptic mange infestation level in one humped camels (Camelus dromedarius), and to assess the effect of the age and season on the mange infestation, to assess the effect of the mange infestation on the appetite and body condition score (BCS) and to develop a good an applicable treatment protocol. 250 female camels were examined clinically and parasitologically, skin scraping revealed that Sarcoptes scabiei var. cameli mite was found in 28% of the examined animals, data analysis of the selected parameters showed that there was an effect for the age and season on the mange infestation, on the other hand there was no effect for the mange infestation on the appetite and BCS. Administration of Doramectin injection and Deltamethrin spray together founded to be the best treatment protocol for the sarcoptic mange infestation in camels, this finding indicates that using the acaricides alone is not enough for the disease eradication and must combined with treatment of the contaminated environment to prevent the reinfection from surrounding arears and achieve the highest cure level.

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Lung cancer remains incurable; therefore, novel therapeutical approaches are ofgreat demand. This study was designed to investigate the effectiveness of cisplatin nanoparticles combined with vitamin-D3 on urethane-induced early lung cancer in rats and to clarify the underlying signaling mechanisms. Early lung cancer was induced in male Wistar rats by urethane. Rats were divided into six groups:I-control, II-cancer untreated, III-cancer + free cisplatin, IV-cancer + cisplatin nanoparticles, V-cancer + free cisplatin + vitamin-D3, VI-cancer + cisplatin nanoparticles + vitamin-D3. Inflammation, proliferation, and apoptosis were evaluated together with the levels of tumor marker CK-19 along with histological assessment. Treatment of lung cancer with either free or nanoparticles of cisplatin alone demonstrated significant suppression in the expression of inflammatory, anti-apoptotic and tumor markers compared to rats with lung cancer. Moreover, vitamin-D3 supplementation with either cisplatin forms lead to a further decrease of all markers, markedly with cisplatin nanoparticles. The presentstudy shows the synergistic effect of cisplatin-nanoparticles combined with vitamin-D3 as a new therapy regimen against lung cancer. Further studies with larger sample sizes and longer duration are needed to confirm these results.

Lung cancer remains incurable; therefore, novel therapeutical approaches are ofgreat demand. This study was designed to investigate the effectiveness of cisplatin nanoparticles combined with vitamin-D3 on urethane-induced early lung cancer in rats and to clarify the underlying signaling mechanisms. Early lung cancer was induced in male Wistar rats by urethane. Rats were divided into six groups:I-control, II-cancer untreated, III-cancer + free cisplatin, IV-cancer + cisplatin nanoparticles, V-cancer + free cisplatin + vitamin-D3, VI-cancer + cisplatin nanoparticles + vitamin-D3. Inflammation, proliferation, and apoptosis were evaluated together with the levels of tumor marker CK-19 along with histological assessment. Treatment of lung cancer with either free or nanoparticles of cisplatin alone demonstrated significant suppression in the expression of inflammatory, anti-apoptotic and tumor markers compared to rats with lung cancer. Moreover, vitamin-D3 supplementation with either cisplatin forms lead to a further decrease of all markers, markedly with cisplatin nanoparticles. The presentstudy shows the synergistic effect of cisplatin-nanoparticles combined with vitamin-D3 as a new therapy regimen against lung cancer. Further studies with larger sample sizes and longer duration are needed to confirm these results.

Lung cancer remains incurable; therefore, novel therapeutical approaches are ofgreat demand. This study was designed to investigate the effectiveness of cisplatin nanoparticles combined with vitamin-D3 on urethane-induced early lung cancer in rats and to clarify the underlying signaling mechanisms. Early lung cancer was induced in male Wistar rats by urethane. Rats were divided into six groups:I-control, II-cancer untreated, III-cancer + free cisplatin, IV-cancer + cisplatin nanoparticles, V-cancer + free cisplatin + vitamin-D3, VI-cancer + cisplatin nanoparticles + vitamin-D3. Inflammation, proliferation, and apoptosis were evaluated together with the levels of tumor marker CK-19 along with histological assessment. Treatment of lung cancer with either free or nanoparticles of cisplatin alone demonstrated significant suppression in the expression of inflammatory, anti-apoptotic and tumor markers compared to rats with lung cancer. Moreover, vitamin-D3 supplementation with either cisplatin forms lead to a further decrease of all markers, markedly with cisplatin nanoparticles. The presentstudy shows the synergistic effect of cisplatin-nanoparticles combined with vitamin-D3 as a new therapy regimen against lung cancer. Further studies with larger sample sizes and longer duration are needed to confirm these results.