Feline parvovirus infection (FPV) is one of the serious diseases in Kittens that causes substantial morbidity and death. For the treatment of affected cats and the prevention of disease spread, early diagnosis of FPV infection is critical. To our knowledge, there have been no reports about the disease’s situation in Egypt’s Assiut province. As a result, the goal of this study was to find out how common FPV infection is among ill cats in this province. A total of 30 cats suspected of being infected with FPV were screened using an antigen rapid test to determine whether they were clinically suspicious. To determine the prevalence of FPV, each investigated cat’s age, sex, breed, season, lifestyle (whether kept indoors or outdoors), and immunization were all documented. Overall, 26.7% of examined cats were affected. FPV infection was more common in young, unvaccinated cats who lived outdoor. Epizootiological monitoring of the prevalence rate based on cat breeds and sex revealed no statistically significant differences. In terms of season, spring had the highest infection rate (57.1%), followed by winter (33.3%), and autumn (7.69%). The rapid one-step test is a useful diagnostic tool for detecting FPV, which was found in the research area’s cats.