Campylobacter is considered among the most important pathogens reported as a cause of bacterial enteritis in human. Epidemiological evidence has linked Campylobacter infection in human with poultry and poultry products. One hundred and eighty random samples from broiler carcasses and some poultry products including cecal contents, muscles, liver, gizzard, chicken minced meat and chicken luncheon samples ( 30 each) were aseptically collected from local poultry slaughter shops and supermarkets at different districts in Assiut province at the period from February to April 2004.These samples were examined for the occurrence of Campylobacter species. The obtained results indicated that Campylobacter species were isolated from 39.17% and 10% of the examined broiler carcasses and poultry products , respectively. Campylobacter species were recovered from muscles, liver and cecal contents with a rate of 53.33% , 20% and 83.33%, respectively. C. jejuni was detected in muscles (16.7%), liver (3.33%), cecal contents (46.7%) and chicken minced meat (20% ), meanwhile C. coli was determined in muscles (36.7%), liver (16.7%) and cecal contents (36.7%). Antibiotic resistance patterns were determined for C. jejuni isolates obtained from muscles, liver and chicken minced meat. In additio’’’n plasmid profile were performed to correlate between antibiotic resistance and plasmid carriage among these isolates. It was found that 75% of C. jejuni isolates obtained from muscles, liver and chicken minced meat showed resistance to ampicillin, followed by 50% of the strains were resistant to chloramphenicol in addition 25% of the strains were resistant to erythromycin, gentamycin and tetracycline. Correlation between plasmid profile analysis and antibiotic resistance of the examined strains were discussed. Public health hazard of multiple antibiotic resistant enteropathogenic Campylobacter was discussed and suggestive measures for reduction of Campylobacter in broilers and poultry products were explained.

Brucellosis is the most important zoonotic disease constituting a public health problem in Assiut Governorate, hence this study was carried out to determine the prevalence of brucellosis among humans in Assiut Governorate. A total of 7154 peripheral blood samples were collected from patients with fever at Assiut Fever Hospital during the period from 2002-2003. A full detailed anamnestic and clinical assessment in the form of questionnaire was designed for each individual to determine the risk factors with specific emphasis to age, sex, residence and occupation. All serum samples were screened for Brucella antibodies by slide agglutination test. Positive sera were further analyzed by standared tube agglutination test. Enzyme linked immunosorbent assay (ELISA) was carried out to detect IgM and IgG Brucella antibodies. Statistical analysis was performed and correlation coefficient was done between all risk factors. Results declared that the prevalence of brucellosis was (1.29 +/- 0.004 %) and (1.22 +/- 0.002 %) as detected by agglutination and ELISA, respectively. IgM antibodies were estimated in 9.8 % of the examined patients, while IgG antibodies were found in 30.4 % of the examined patients, moreover both IgM and IgG antibodies were detected in 54.3 % of the examined patients. The prevalence of brucellosis was significantly (P 0.05) affected by sex, where the rate of detection was higher among females (1.76 +/- 0.009 %) than males (1.05 +/- 0.004 %) as detected by agglutination test. On the other hand, the prevalence rate based on ELISA was (1.64 +/- 0.39 % and 1.01 +/- 0.89 %) for females and males, respectively. Prevalence of brucellosis was higher in rural areas (1.3 +/- 0.005 % & 1.25 +/- 0.009 %) than in urban areas (1.23 +/- 0.001% & 1.12 +/- 0.01 %) as detected by agglutination test and ELISA, respectively. The prevalence of brucellosis varied significanctly between different occupational and age groups. Public health impact of brucellosis is discussed and suggestive measures for control are explained.

Brucellosis is the most important zoonotic disease constituting a public health problem in Assiut Governorate, hence this study was carried out to determine the prevalence of brucellosis among humans in Assiut Governorate. A total of 7154 peripheral blood samples were collected from patients with fever at Assiut Fever Hospital during the period from 2002-2003. A full detailed anamnestic and clinical assessment in the form of questionnaire was designed for each individual to determine the risk factors with specific emphasis to age, sex, residence and occupation. All serum samples were screened for Brucella antibodies by slide agglutination test. Positive sera were further analyzed by standared tube agglutination test. Enzyme linked immunosorbent assay (ELISA) was carried out to detect IgM and IgG Brucella antibodies. Statistical analysis was performed and correlation coefficient was done between all risk factors. Results declared that the prevalence of brucellosis was (1.29 +/- 0.004 %) and (1.22 +/- 0.002 %) as detected by agglutination and ELISA, respectively. IgM antibodies were estimated in 9.8 % of the examined patients, while IgG antibodies were found in 30.4 % of the examined patients, moreover both IgM and IgG antibodies were detected in 54.3 % of the examined patients. The prevalence of brucellosis was significantly (P 0.05) affected by sex, where the rate of detection was higher among females (1.76 +/- 0.009 %) than males (1.05 +/- 0.004 %) as detected by agglutination test. On the other hand, the prevalence rate based on ELISA was (1.64 +/- 0.39 % and 1.01 +/- 0.89 %) for females and males, respectively. Prevalence of brucellosis was higher in rural areas (1.3 +/- 0.005 % & 1.25 +/- 0.009 %) than in urban areas (1.23 +/- 0.001% & 1.12 +/- 0.01 %) as detected by agglutination test and ELISA, respectively. The prevalence of brucellosis varied significanctly between different occupational and age groups. Public health impact of brucellosis is discussed and suggestive measures for control are explained.

Background/Aim: Helicobacter pylori is considered as one of the most common chronic bacterial zoonotic infections worldwide. H. pylori infection constitutes a public health hazard problem with a fatal sequele. This study was designed to estimate the prevalence of H. pylori among children and adults in Assiut Governorate in Upper Egypt and to evaluate the risk factors affecting the increase of infection. Methods: A total of 200 peripheral blood samples constituting 50 samples from children and 150 samples from adults were collected from hospital patients. A full detailed anamnestic and clinical assessment in the form of questionnaire was designed for each patient to determine the risk factors with specific emphasis to age, sex and residence. Seroprevalence of H. pylori IgG antibodies was evaluated by using enzyme linked immunosorbent assay. Results: We estimated a seroprevalence rate of 75.5%. H. pylori exposure was higher in adults (79.33%) than in children (64%) and it was statistically significant. The seroprevalence of infection was higher in rural areas (87.3%) than in urban areas (40%). Conclusion: H.pylori infection was prevalent among humans in the areas studied. Public health hazard of H. pylori infection and preventive measures to control the infection were discussed