Serial concentrations of aflatoxin B1 ranged from 200 to 1000 p.p.b. were assayed for detoxification by acidogenous yoghurt. Thin-layer chromatography analysis revealed a complete transformation of 800 p.p.b. of aflatoxin B1 to a new fluorescing compound corresponding to aflatoxin B2a which is referred as hydroxydihydroaflatoxin B1. Partial conversion was present in yoghurt sample containing 1000 p.p.b. Toxicity test on chickens, confirmed Ciegler findings

The main aim of this study was to determine the effect of aflatoxin on male reproduction by exposure to fodders contaminated by moulds producing toxins as a natural environmental contaminants. Semen was collected from three normal healthy buffalo bulls every ten days for one month before adding the toxin and examined for livability and abnormality percentages. Moldy rice containing aflatoxin B and G was incorporated into the ration of the studied bulls in a daily amount of 2.0, 3.0 and 4.0 gm respectively for each bull for two weeks. Each gramme of the rice powder contained 15.6 ppm aflatoxin B and 9.84 ppm aflatoxin G as determined spectrophotometrically. Semen was collected in the middle of the two weeks experimental period. Moreover, one week, one month and two months later, semen was collected again and examined for the same parameters. The results showed marked decrease in the alive percentage of spermatozoa from 91.11% to 80.8% after the addition of aflatoxin. Moreover, a very high increase in the sperm abnormalities up to 54% was recorded

The present study aimed at: 1. Examination of commercial chicken and duck eggs for the possible presence of aflatoxins. 2. Study of the sex susceptibility of mature chickens when fed on ration containing aflatoxins. 3. Study of clinicopathological effects on their ovaries and testicles. In this investigation, 100 chicken and 50 duck eggs collected from markets in the vicinity of Assiut Governorate, were examined individually for the presence of aflatoxins in the whites and yolks. The study revealed that the examined eggs contained no aflatoxins. Experimentally, aflatoxin BV(8.1 ppm) and G. (1.6 ppm) were incorporated into the feed of five laying hens and five mature cooks for three weeks duration. The layers showed cessation of egg production during the whole feeding period. Histopathological examinations revealed that, the ovaries showed follicular atresia while testicles were normal. It was shown that, aflatoxicosis cause pathological changes in the chicken ovaries, which has a detrimental effect on egg production

A wide range of fungi, amounting to fifty-six species belonging to twenty-two genera, have been recovered from animal feeds and foodstuffs. The most frequent fungi were Aspergillus niger, and A. flavus, followed by Mucor racemosus, Alternaria alternata. Rhizopus stolonifer, Penicillium corylophilum and P. notatum. Three genera were found to be of moderate occurrence, namely, Mucor, Rhizopus and alternaria. The three following genera were of low occurrence: cladosporium, Fusarium, and Neurospora. The fluorescence method of detecting aflatoxin-producing strains demonstrated that only one is isolated of A. flavus possesses this property. Certain species of Penicillium and Aspergillus produced fluorescent substances (metabolites) similar in color to B and G aflatoxin. These substances were subsequently proved not to be aflatoxin by (TLC) chromatography. The animal and public health significance from such toxins was also discussed.