A total of 100 samples of Domiati and Kareish cheese were randomly collected and examined for the detection of Cronobacter species. Detection was performed using Cronobacter Screening Broth (CSB), Chromogenic Cronobacter Isolation Agar (mDFI) followed by biochemical testing. Cronobacter species were isolated from 8% of total samples. Cronobacter sakazakii was isolated from both Kareish and Domiati cheeses, while, Cronobacter malonaticus and Cronobacter turicensis were isolated only from Kareish cheese. The identified isolates of Cronobacter sakazakii were further tested by real time PCR. The results indicate that Kareish and Domiati cheeses represent risk for humans and further attention should be paid for manufacturing and handling of such cheeses.

A total of 100 samples of Domiati and Kareish cheese were randomly collected and examined for the detection of Cronobacter species. Detection was performed using Cronobacter Screening Broth (CSB), Chromogenic Cronobacter Isolation Agar (mDFI) followed by biochemical testing. Cronobacter species were isolated from 8% of total samples. Cronobacter sakazakii was isolated from both Kareish and Domiati cheeses, while, Cronobacter malonaticus and Cronobacter turicensis were isolated only from Kareish cheese. The identified isolates of Cronobacter sakazakii were further tested by real time PCR. The results indicate that Kareish and Domiati cheeses represent risk for humans and further attention should be paid for manufacturing and handling of such cheeses.

A total of one hundred and fifty milk-based desserts samples including rice with milk, pudding, and custard (50 each) were collected from different localities in Assiut city, Egypt. The collected samples were examined for the isolation and enumeration of Bacillus cereus. The Most Probable Number technique was used for the enumeration of Bacillus cereus using tryptone soy polymyxin broth and Mannitol Egg Yolk Polymyxin (MYP) agar. The incidence of Bacillus cereus was 62%, 44% and 32% in the examined rice with milk, pudding, and custard samples, respectively. The Bacillus cereus count in most of the examined samples was in the range of 10-102 CFU/g. The public health importance of Bacillus cereus was also discussed.

Two hundred and forty random samples of milk and some dairy products; Domiati, Kareish cheese and cream were collected from Assiut city, Egypt. The collected samples were examined for the incidence of Acinetobacter species using Leeds Acinetobacter selective medium. The obtained results revealed that Acinetobacter species was isolated from 15% of the raw milk samples, 3.3% of the Domiati cheese samples, 13.3% of the Kareish cheese samples and 13.3% of the cream samples, while couldn’t be detected in the pasteurized milk samples. The isolated species were Acinetobacter baumannii, Acinetobacter haemolyticus, Acinetobacter calcoaceticus and Acinetobacter junii. Acinetobacter baumannii was detected using blaOXA-23-like, blaOXA-51-like, and class 1 integrase genes. The characterization of the Acinetobacter species for the production of lipolytic enzyme was studied. Out of the 27 isolated Acinetobacter species, 23 possessed lipolytic activity. The public health hazard of this microorganism in milk and some dairy products was discussed.

Two hundred and forty random samples of milk and some dairy products; Domiati, Kareish cheese and cream were collected from Assiut city, Egypt. The collected samples were examined for the incidence of Acinetobacter species using Leeds Acinetobacter selective medium. The obtained results revealed that Acinetobacter species was isolated from 15% of the raw milk samples, 3.3% of the Domiati cheese samples, 13.3% of the Kareish cheese samples and 13.3% of the cream samples, while couldn’t be detected in the pasteurized milk samples. The isolated species were Acinetobacter baumannii, Acinetobacter haemolyticus, Acinetobacter calcoaceticus and Acinetobacter junii. Acinetobacter baumannii was detected using blaOXA-23-like, blaOXA-51-like, and class 1 integrase genes. The characterization of the Acinetobacter species for the production of lipolytic enzyme was studied. Out of the 27 isolated Acinetobacter species, 23 possessed lipolytic activity. The public health hazard of this microorganism in milk and some dairy products was discussed.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of heat stable toxin (STh) and heat labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.