Dimethoate (DM) and Lambda cyhalothrin (LCT) are commonly used insecticides. Human being and farm animals are expected to have acute toxicity. The present work aimed to explore the effect of acute exposure to DM and LCT on hematological parameters and to detect DNA damage in bone marrow of Sprague Dawley rats using the alkaline single cell gel electrophoresis assay (comet assay). Thirty animals were divided into three groups of ten rats each. LCT group administered 26 mg/kg body weight, DM group administered 103 mg/kg body weight orally for 24 and 48 hours, while the control group received the vehicle only. Blood samples were collected for hematological analysis, bone marrow was flushed from the femur bone for comet assay and spleen samples were preserved in formalin for histopathological examination. Results showed minor changes in blood profile in all exposed groups associated with mild changes in histology of spleen tissue. Alkaline single cell gel electrophoresis assay in bone marrow cells showed that LCT and DM caused extensive and severe DNA damage after 48 h exposure expressed as significant increases in all comet parameters (% DNA in tail, tail length, tail moment and Olive tail moment). The results concluded that LCT and DM induced DNA damage in bone marrow of rats, LCT showed higher degree of DNA damage in comparison with DM.

Dimethoate (DM) and Lambda cyhalothrin (LCT) are commonly used insecticides. Human being and farm animals are expected to have acute toxicity. The present work aimed to explore the effect of acute exposure to DM and LCT on hematological parameters and to detect DNA damage in bone marrow of Sprague Dawley rats using the alkaline single cell gel electrophoresis assay (comet assay). Thirty animals were divided into three groups of ten rats each. LCT group administered 26 mg/kg body weight, DM group administered 103 mg/kg body weight orally for 24 and 48 hours, while the control group received the vehicle only. Blood samples were collected for hematological analysis, bone marrow was flushed from the femur bone for comet assay and spleen samples were preserved in formalin for histopathological examination. Results showed minor changes in blood profile in all exposed groups associated with mild changes in histology of spleen tissue. Alkaline single cell gel electrophoresis assay in bone marrow cells showed that LCT and DM caused extensive and severe DNA damage after 48 h exposure expressed as significant increases in all comet parameters (% DNA in tail, tail length, tail moment and Olive tail moment). The results concluded that LCT and DM induced DNA damage in bone marrow of rats, LCT showed higher degree of DNA damage in comparison with DM.

Aluminum (Al) is a trace element available in the Earth’s crust naturally and also is the most toxic metals studied because it caused many affections in animals and human. It has been suggested as a contributing factor in the pathogenesis of encephalopathy, osteomalacia and microcytic anemia. So it causes many economical losses. Selenium (Se) is an essential trace mineral of primary importance for human and animal health. Vitamin E compounds and Selenium haveantioxidative characteristics which needed for the suitable function of the immune system. Meso-2,3-dimercaptosuccinic acid (DMSA) is a provocative chelation which bound to plasma albumin and appears to be excreted in the urine. Therefore, the present study has been designed to explore the protective effects of vitamin E & selenium and/ or DMSA against aluminum chloride intoxication in rats by monitoring hematological picture, serum testosterone hormone, Aluminum residues, lipid per oxidation, Nitric oxid, some antioxidant enzymes as catalase (CAT), glutathione peroxides (GPx) in brain and testicular tissues beside the pathological examination. 50 adult male albino rats were divided into five groups. The first group (group I) served as a control. The second group exposed to Aluminumchloride (AlCl3) twice weekly at a dose of 2 mg/kg b.w, twice / week, orally by stomach tube for three months. The third group received DMSA at a dose of 27 mg/kg b.w, twice/ week, orally by stomach tube in addition to AlCl in the same dose for 3 months. The fourth group (group IV) received Vitamin E & Selenium (1ml/ liter) in drinking water in addition to AlCl3 at the same dose for 3 months. The fifth group (group V) received Vitamin E & Selenium in drinking water and DMSA by stomach tube in addition to AlCl3 at the same doses for 3 months. Blood samples were taken for complete blood picture (CBC) also serum was obtained for determination of testosterone hormone levels as well as brain and testes tissue for biochemical parameters, AL residues estimation, and pathological examination . The results of hematological picture revealed that animals in group II showed a significant increase in WBCs, monocytes, lymphocytes, granulocytes and platelets when compared with other groups, while RBCs count significantly decreased. There was a highly significant increase in lipid per oxidation (MDA) and Nitric oxid in group II while GPx and CAT levels were significantly decreased. Rats in group II have a significantly higher concentration of AL in brain and testes tissue than in other treated groups specially group III and V. The histopathological examination of brain in group II showed demyelination, neuronal degeneration up to necrosis compared to other groups as well as degeneration of spermatogenic cell in the somniferous tubules of testes with formation of spermatid giant cells inside the lumen with interstitial inflammation and degeneration of pseudo stratified columnar epithelium of prostate gland. The results of this investiga- tion demonstrated that aluminum chloride toxicity induces morph pathological lesions in brain, testes and prostate gland. It decreases GPx and CAT activates. Vitamin E and selenium with DMSA have the best ameliorating effect against Al toxicity after three months post-treatment.

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Abstract Go to The lack of a national campylobacteriosis surveillance system in Egypt warrants the need for periodical disease evaluation. This study aimed to determine campylobacteriosis prevalence in broiler chicken farms and slaughterhouses as well as in humans at Assiut Governorate, Egypt. Biochemical and molecular reactions have been employed to determine Campylobacterioiss prevalence. Additionally, the predisposing factors for human campylobacteriosis were assessed. The overall prevalence of Campylobacter species detected by biochemical reactions and multiplex PCR assay was found to be 23.51% and 22.46%, respectively. Prevalence rates of 16.83%, 24% and 27.55% were recorded for the incorporated samples from broiler farms, slaughterhouses and humans, respectively. None of the analyzed demographic determinants (age, gender and residence) significantly affected Campylobacteriois prevalence in humans. Interestingly, mixed infection was the predominate finding among positive samples and none was confirmed to have C. jejuni alone. Additionally, Arcobacter was recovered either independently or coexisted with Campylobacter in poultry samples; nevertheless, zero detected from humans. This study confirms that broilers could represent an important public health threat to Assiut Governorate residents. Accordingly, measures on how to minimize the contamination level at farms, slaughterhouses and during culinary practices should be disseminated to farm workers, slaughterhouse men and consumers.

Abstract Go to The lack of a national campylobacteriosis surveillance system in Egypt warrants the need for periodical disease evaluation. This study aimed to determine campylobacteriosis prevalence in broiler chicken farms and slaughterhouses as well as in humans at Assiut Governorate, Egypt. Biochemical and molecular reactions have been employed to determine Campylobacterioiss prevalence. Additionally, the predisposing factors for human campylobacteriosis were assessed. The overall prevalence of Campylobacter species detected by biochemical reactions and multiplex PCR assay was found to be 23.51% and 22.46%, respectively. Prevalence rates of 16.83%, 24% and 27.55% were recorded for the incorporated samples from broiler farms, slaughterhouses and humans, respectively. None of the analyzed demographic determinants (age, gender and residence) significantly affected Campylobacteriois prevalence in humans. Interestingly, mixed infection was the predominate finding among positive samples and none was confirmed to have C. jejuni alone. Additionally, Arcobacter was recovered either independently or coexisted with Campylobacter in poultry samples; nevertheless, zero detected from humans. This study confirms that broilers could represent an important public health threat to Assiut Governorate residents. Accordingly, measures on how to minimize the contamination level at farms, slaughterhouses and during culinary practices should be disseminated to farm workers, slaughterhouse men and consumers.

Abstract Go to The lack of a national campylobacteriosis surveillance system in Egypt warrants the need for periodical disease evaluation. This study aimed to determine campylobacteriosis prevalence in broiler chicken farms and slaughterhouses as well as in humans at Assiut Governorate, Egypt. Biochemical and molecular reactions have been employed to determine Campylobacterioiss prevalence. Additionally, the predisposing factors for human campylobacteriosis were assessed. The overall prevalence of Campylobacter species detected by biochemical reactions and multiplex PCR assay was found to be 23.51% and 22.46%, respectively. Prevalence rates of 16.83%, 24% and 27.55% were recorded for the incorporated samples from broiler farms, slaughterhouses and humans, respectively. None of the analyzed demographic determinants (age, gender and residence) significantly affected Campylobacteriois prevalence in humans. Interestingly, mixed infection was the predominate finding among positive samples and none was confirmed to have C. jejuni alone. Additionally, Arcobacter was recovered either independently or coexisted with Campylobacter in poultry samples; nevertheless, zero detected from humans. This study confirms that broilers could represent an important public health threat to Assiut Governorate residents. Accordingly, measures on how to minimize the contamination level at farms, slaughterhouses and during culinary practices should be disseminated to farm workers, slaughterhouse men and consumers.

This study aimed to establish normal ultrasonographic reference values of kidney dimensions in donkeys (Equus asinus) and to describe and evaluate the clinicopathological variations associated with ultrasound-guided renal biopsy. The ultrasonographic dimensions of the right and left kidneys were conducted on 16 donkeys, which were then divided into two groups; eight each for biopsy of the right kidney (RK) and left kidney (LK). Three ultrasonographic cineloops were obtained at 17th intercostal space daily for 3 consecutive days. Renal length, width, and dimensions of the cortex, medulla, and pelvis for both the kidneys in each donkey were recorded. Maximal dimensions were obtained for the RK (length 10 ± 8 cm, width 4.9 ± 1 cm, thickness 4.2 ± 0.4 cm) and LK (length 8.9 ± 0.9 cm, width 4.7 ± 0.8 cm, thickness 3.5 ± 0.7 cm) with good-to-excellent repeatability for all measurements. Follow-up ultrasonography revealed development of postbiopsy subcapsular hematomas, which were confirmed postmortem, of mild (volume 20 mL), moderate (volume from 20 to 40 mL), and severe degrees (volume > 40 mL). Gross hematuria had been observed till 24 hours after biopsy, and then microscopic hematuria was noticed thereafter. Variable clinicopathological changes were noticed in blood and urine. All the biopsy specimens were adequate for histopathological assessment. Postmortem histopathological examination revealed various kidney changes. In conclusion, kidney dimensions can be used by veterinarians for accurate diagnosis and management of renal diseases. Ultrasound-guided renal biopsy is a relatively safe procedure; however, some complications may develop. Renal biopsy is commonly associated with clinicopathological variations; thus, caution should be taken during interpretation of these variables.