Aim: Evaluate the effect of omega-3 fatty acids( ω−3) on serum lipids (cholesterol and triglycerides) and blood pressure level in osteoarthritis (OA)patients. Patients& Methods: 200 female OA patients from Assiut University Hospitals, were diagnosed according the American College of Rheumatology Criteria( ACR). They were submitted to socioeconomic history taking, clinical, Rheumatological examination and laboratory investigations. They were equally devided to four groups as follow: group(1): patients treated with physiotherapy only (control group). (2): patients treated with physiotherapy + omega-3 fatty acids (3capsules/day). (3): patients treated with physiotherapy + (5gm/day linseed oil). (4): patients treated with physiotherapy + (350 gm/week) fish or salamon. Statistical analysis and nutrition education were applied. Results: revealed that mean level of cholesterol was significantly reduced in the participiant OA patients at (P0.01, P0.000, P0.05) . Also serum triglycerides concentration was significantly decreased in patients taking ω−3 fatty acids as linseed oil and fish (groups 3&4).The erthrocyte sedimentation rate (ESR) was significant decreased in OA patients taking ω−3 fatty acids (groups2,3,4). Omega-3 fatty acids from capsules, or linseed oil or fish decreased the systolic blood pressure. Also it was clear that knee joints which are affected were the most common, while hip joints were the least.