The morphological features, phytochemical composition and biological activities of Pteris vittata L., growing rarely in Egypt were investgated. The morphological features of P. vittata including pale brown scales, short creeping rhizome and different features of fronds and spores were investigated. A number of chemical compounds related to different families were screened for the first time in the hexane extract of P. vittata by the GC-MS method. Fourteen phenolic compounds including pyrogallol, coumaric acid, ellagic acid, ferulic acid and chlorogenic acid were identified by HPLC on methanol: acetone: H2O (2:2:1) extract of P. vittata.