Synthesis of some new derivatives of Novel l-Alkyl-6-hydroxyperhydro-1,4-diazepine-2,3-diones. The chemical structure of the target compounds was confirmed using IR, NMR, MS analyses in addition to elemental microanalyses. The target compounds were test for their pharmacological activities.

Synthesis of some new derivatives of Novel l-Alkyl-6-hydroxyperhydro-1,4-diazepine-2,3-diones. The chemical structure of the target compounds was confirmed using IR, NMR, MS analyses in addition to elemental microanalyses. The target compounds were test for their pharmacological activities.

Synthesis of some new derivatives of Novel l-Alkyl-6-hydroxyperhydro-1,4-diazepine-2,3-diones. The chemical structure of the target compounds was confirmed using IR, NMR, MS analyses in addition to elemental microanalyses. The target compounds were test for their pharmacological activities.

Highly selective spectrofluorimetric detection of Fe3+ ions in acidic medium is developed using novel fluorescent sensor, namely 4-(2-(3-Methyl-5-oxo-1-tosyl-1H-pyrazol-4(5H)-ylidene) hydrazinyl)-N- (pyrimidin-2-yl) benzenesulfonamide (PHBS-sensor), which gives an selective fluorescence quenching response at λEmission 379 nm. The factors affecting fluorescence detection process, such as the pH, solvent type, buffer type, probe concentration, reaction time and co-existing ions were systematically studied and optimized. Theoretical modeling of Fe3+ monitoring through DFT calculation was investigated to display the mechanism of PHBS-Fe3+ complexation. The proposed PHBS-sensor can detect Fe3+ ions at ultra-trace levels with limit of detection 1.7 × 10−5 M. Moreover, this applied method was effectively utilized for the monitoring/detection of Fe3+ ions in real water, such as ground, tap and Nile river water, and pharmaceutical samples, such as hard gelatin capsule and syrup.

Highly selective spectrofluorimetric detection of Fe3+ ions in acidic medium is developed using novel fluorescent sensor, namely 4-(2-(3-Methyl-5-oxo-1-tosyl-1H-pyrazol-4(5H)-ylidene) hydrazinyl)-N- (pyrimidin-2-yl) benzenesulfonamide (PHBS-sensor), which gives an selective fluorescence quenching response at λEmission 379 nm. The factors affecting fluorescence detection process, such as the pH, solvent type, buffer type, probe concentration, reaction time and co-existing ions were systematically studied and optimized. Theoretical modeling of Fe3+ monitoring through DFT calculation was investigated to display the mechanism of PHBS-Fe3+ complexation. The proposed PHBS-sensor can detect Fe3+ ions at ultra-trace levels with limit of detection 1.7 × 10−5 M. Moreover, this applied method was effectively utilized for the monitoring/detection of Fe3+ ions in real water, such as ground, tap and Nile river water, and pharmaceutical samples, such as hard gelatin capsule and syrup.

Pseudomonas (Ps.) aeruginosa is one of the most common pathogens causing nosocomial infections. This pathogen causes several infections including urinary tract infection (UTI), wound infection, pneumonia, bacteremia,…etc. Immunocompromised patients and patients in intensive care unit are at high risk of acquisition of infection, in addition multidrug resistant Ps. aeruginosa isolates had been characterized. This study was planned to determine the frequency of Ps. aeruginosa in nosocomially infected patients in Assiut university hospital and to type isolated strains. In this study, 677 samples collected from 366 nosocomially infected patients admitted to different hospital wards at Assiut University Hospitals, including chest, trauma, neurology, internal medicine, post operative and pediatric ICUs, trauma and hematology units. Identification of bacterial strains was done by cultural and biochemical tests. Biotyping analysis for isolated strains was done using API 20NE. In our study, a total of 30 (8.2%) Ps. aeruginosa strains were isolated. Four API codes profile for Ps. aeruginosa isolates were identified, the isolate with API code profile 1154575 was more frequent distributed in Assiut University Hospital.

Pseudomonas (Ps.) aeruginosa is one of the most common pathogens causing nosocomial infections. This pathogen causes several infections including urinary tract infection (UTI), wound infection, pneumonia, bacteremia,…etc. Immunocompromised patients and patients in intensive care unit are at high risk of acquisition of infection, in addition multidrug resistant Ps. aeruginosa isolates had been characterized. This study was planned to determine the frequency of Ps. aeruginosa in nosocomially infected patients in Assiut university hospital and to type isolated strains. In this study, 677 samples collected from 366 nosocomially infected patients admitted to different hospital wards at Assiut University Hospitals, including chest, trauma, neurology, internal medicine, post operative and pediatric ICUs, trauma and hematology units. Identification of bacterial strains was done by cultural and biochemical tests. Biotyping analysis for isolated strains was done using API 20NE. In our study, a total of 30 (8.2%) Ps. aeruginosa strains were isolated. Four API codes profile for Ps. aeruginosa isolates were identified, the isolate with API code profile 1154575 was more frequent distributed in Assiut University Hospital.

Pseudomonas (Ps.) aeruginosa is one of the most common pathogens causing nosocomial infections. This pathogen causes several infections including urinary tract infection (UTI), wound infection, pneumonia, bacteremia,…etc. Immunocompromised patients and patients in intensive care unit are at high risk of acquisition of infection, in addition multidrug resistant Ps. aeruginosa isolates had been characterized. This study was planned to determine the frequency of Ps. aeruginosa in nosocomially infected patients in Assiut university hospital and to type isolated strains. In this study, 677 samples collected from 366 nosocomially infected patients admitted to different hospital wards at Assiut University Hospitals, including chest, trauma, neurology, internal medicine, post operative and pediatric ICUs, trauma and hematology units. Identification of bacterial strains was done by cultural and biochemical tests. Biotyping analysis for isolated strains was done using API 20NE. In our study, a total of 30 (8.2%) Ps. aeruginosa strains were isolated. Four API codes profile for Ps. aeruginosa isolates were identified, the isolate with API code profile 1154575 was more frequent distributed in Assiut University Hospital.

Pseudomonas (Ps.) aeruginosa is one of the most common pathogens causing nosocomial infections. This pathogen causes several infections including urinary tract infection (UTI), wound infection, pneumonia, bacteremia,…etc. Immunocompromised patients and patients in intensive care unit are at high risk of acquisition of infection, in addition multidrug resistant Ps. aeruginosa isolates had been characterized. This study was planned to determine the frequency of Ps. aeruginosa in nosocomially infected patients in Assiut university hospital and to type isolated strains. In this study, 677 samples collected from 366 nosocomially infected patients admitted to different hospital wards at Assiut University Hospitals, including chest, trauma, neurology, internal medicine, post operative and pediatric ICUs, trauma and hematology units. Identification of bacterial strains was done by cultural and biochemical tests. Biotyping analysis for isolated strains was done using API 20NE. In our study, a total of 30 (8.2%) Ps. aeruginosa strains were isolated. Four API codes profile for Ps. aeruginosa isolates were identified, the isolate with API code profile 1154575 was more frequent distributed in Assiut University Hospital.

Disturbances in autophagy are known to be implicated in autoimmune disorders. Many studies have connected polymorphisms in autophagy-related gene 5 (ATG-5) to systemic lupus erythematosus (SLE). Our aim was the determination of the expression level of ATG-5, Beclin-1 and microtubule-associated protein-light chain 3 (LC- 3) in Egyptian SLE patients to investigate the impact of disturbances in autophagy genes on the incidence and progression of the disease. Also, we investigated the incidence of single nucleotide polymorphism (SNP) rs573775 in ATG-5 gene among Egyptian SLE patients. Our results showed that the mean levels of Beclin-1, LC-3 and interleukin (IL)-10 transcripts were significantly higher in SLE patients compared to healthy controls. The previous transcripts were positively correlated with SLE Disease Activity Index (SLEDAI). Beclin-1 and LC-3 transcripts were negatively correlated to complement component 3 (C3) levels. Only LC-3 transcripts were negatively correlated to complement component 4 (C4). The rs573775 SNP of ATG-5 with the variant allele was significantly associated with disease susceptibility, conferring a higher risk of SLE development. This variant allele was more prevalent in patients below 30 years, patients with anemia and in patients with anti-double-stranded DNA (dsDNA), confirming the essential role of ATG-5 polymorphism in the susceptibility of Egyptian patients to SLE.