Ethnopharmacological relevance

Although burn wound management in medical practice has improved, the challenge still exists and demands multidisciplinary participation. Wheat has been extensively used as a remedy of different skin conditions especially burns in almost all parts of Italy. However, the mechanism is still unclear.

Aim of the study

To examine the effects of different wheat bran extracts on the healing of burn wound estimated grossly, histopathologically, and immunologically.

Materials and methods

Wistar male rats were divided into 5 groups; (control) saline treated group, Mebo (Moist Exposed Burn Ointment), cold water extract, hot water extract and methanolic extract groups. After the burn, four groups from the experimental groups were treated with Mebo, cold water extract, hot water extract and methanolic extract, respectively, but the negative-control group was treated daily with saline only. The observation was made along days 0, 5, 9, 13, 17 and 21. At the end of the study, rats were sacrificed, and blood samples were collected for biochemical and cytokines estimation in addition to wound samples were collected for histopathological and immunohistochemical studies.

Results

The histopathological examination showed that the inflammation was significantly decreased in all extract-treated groups when compared with saline and Mebo-treated groups. Epithelial tissue and granulation tissue formation most significantly appeared in the cold water extract-treated group.

Conclusions

Cold water and methanolic extract groups showed the most rapid and effective healing of the burn wound. Particularly the cold water extract, due to the sufficient concentration of zinc and phenolic components.

Candida species are one of common opportunistic pathogens in humans. Detection of antifungal resistance between candida species become increasing, a great interest in creating new antifungals utilizing natural sources like plant essential oils (EOs) .

This study aimed to evaluate the effect of EOs of selected plants on Candida species growth, transition, and biofilm formation as well on the expression levels of certain virulence genes.

Patients and methods: Fifty candida isolates were collected from napkin dermatitis and oral candidiasis patients, different Candida species were identified phenotypically. Essential oils for some medicinal plants (basil, clove, garlic, and peppermint) were extracted by hydro-distillation. Their antifungal activity was assessed by minimal inhibitory concentration methods. In addition the effect of EOs on different Candida ultrastructure was detected by scanning electron microscope, the antibiofilm characteristics were examined by microtiter plate method. The ability of tested EOs to interfere with transition of candida from blastopore to hyphae form was evaluated. The effect of basil and clove oils on various virulent genes was determined by analyzing the expression levels of HWP, ALS3, SAP3 genes by RT-PCR.

Results: C. albicans was the most prevalent species. For all tested Candida isolates, the EOs displayed a high antifungal activity. The EOs decreased the Candida transition, disrupted the Candida ultrastructure and suppressed their ability of biofilm formation, clove oil exposure significantly downregulated the expression of HWP1, ALS3 and SAP3 genes, in addition basil oil downregulated the expression of ALS3 and SAP3.

Malaria as an infectious disease is one of the world's most dangerous parasitic diseases. There is an urgent need for the development of new antimalarial drugs. Natural products are a very rich source of new bioactive compounds. Our research aims to shed light on the recent studies which demonstrated the antimalarial potential of phenylpropanoids as a major natural-products class. This study involves an in silico analysis of naturally-occurring phenylpropanoids and phenylethanoids which showed 25 compounds with moderate to strong binding affinity to various amino acid residues lining the active site; P. falciparum kinase (PfPK5), P. falciparum cytochrome bc1 complex (cyt bc1), and P. falciparum lysyl-tRNA synthetase (PfKRS1); of Plasmodium falciparum parasite, a unicellular protozoan which causes the most severe and life-threatening malaria. Furthermore, the study was augmented by the assessment of antiplasmodial activity of glandularin, a naturally occurring dibenzylbutyrolactolic lignan, against chloroquine-sensitive 3D7 strain of P. falciparum using SYBR green I-based fluorescence assay, which showed high antimalarial activity with IC₅₀ value of 11.2 μM after 24 hours of incubation. Our results highlight phenylpropanoids and glandularin in particular as a promising chemical lead for development of antimalarial drugs.