This study aimed to evaluate the impact of specifically designed nursing guidelines on the knowledge and practical skills of nurses caring for patients with burn injuries. A quasi-experimental design with pre-test and post-test assessments was employed. The study was conducted in the burn unit of a Main University Hospital in Egypt. A convenience sample of 20 nurses of various ages and both genders participated. Data were collected using two tools:(I) a structured interview questionnaire to assess demographic data and knowledge, and an observational checklist to evaluate practices; and (II) the Designed Nursing Care Guidelines for Burn Patients (a booklet). The intervention involved the implementation of these guidelines. Following the intervention, a significant improvement was observed in both nurses' knowledge and practical skills. The rate of correct knowledge answers increased from a pre-test mean of 72% to a post-test mean of 89%. The percentage of correctly performed practical tasks rose from 11% to 25%. The most substantial improvements were noted among younger nurses (20-30 years) and those holding a bachelor's degree. Furthermore, nurses with 5-10 years of experience in the burn unit demonstrated the highest overall levels of knowledge and practice post-intervention. The designed nursing guidelines were effective in significantly enhancing both the knowledge and practical skills of nurses providing burn care. To sustain and build upon these improvements, a commitment to continuous education and the standardized use of evidence-based protocols is essential. We recommend the establishment of regular training.