Background: ChatGPT, a language model developed by Open AI, has the potential to play a role in nursing education. This study aims to evaluate effectiveness of ChatGpt as an educational tool for nurses regarding ICU-acquired muscle weakness. Design: A quasi-experimental study design was used. Setting: The study was conducted in general intensive care unit at Sohag main University Hospital. Subjects: Convenient sample of all available nurses working in general ICU. Three tools were used in this study. Tool I: Structured Interview Questionnaire, Part I: Personal and Sociodemographic Data, Part II: Nurses Knowledge Assessment Questionnaire pre &post-test. Tool II: Nurses’ Attitude Assessment Questionnaire: Pré/Post test. Tool III: Feedback from Nurses about ChatGPT Usability and Satisfaction. Results: 77% of the studied nurses had good knowledge versus 23% had inadequate level of knowledge in the Post-test. Also, 72% of the studied nurses revealed satisfied attitude toward using Chat GPT posttest versus 28% of them had unsatisfactory attitude in the post-test. Conclusion: ChatGPT was effective in improving nurses’ knowledge and attitude regarding ICU acquired weakness. Recommendations: Nurses as educators should carefully consider how to integrate ChatGPT into their learning practices.