Blood parasites pose a significant threat to livestock production in southern Egypt, yet there is a scarcity of information regarding their circulation and epidemiology in sheep in this region. This study aimed to investigate the seroprevalence of blood parasite infections in sheep in Assiut governorate, Upper Egypt.

Methods: A total of 400 blood samples were collected from sheep of varying ages and genders. The preliminary screening for the presence of piroplasms, mainly Babesia and Theileria spp., via microscopic examination, followed by investigation of the potential risk factors linked with the exposure to infection. Moreover, molecular identification of both parasites on some of positive samples was performed using PCR targeting Babesia 18S rRNA and Theileria annulata Tams1 gene.

Results: The microscopic examination revealed that among the examined sheep, there was an overall prevalence of blood parasites at 44% (176 out of 400), with Babesia spp. observed in 14% (56 out of 400) and Theileria spp. in 30% (120 out of 400). Furthermore, the infection rate was non-significantly higher in young animals (50%) compared to adults (38.5%) (P = 0.246). Male sheep exhibited a significantly higher vulnerability to both parasites' infection (63.3%) compared to females (35.7%) (P = 0.011). Interestingly, the prevalence of both blood parasites was significantly higher during the cold season (66.1%) compared to the hot season (15.9%) (P = <0.001). The molecular analysis identified the presence of Babesia ovis and Theileria annulata among a subsample of the positive sheep's bloods films. The identified species were recorded in the GenBank™ databases and assigned specific accession numbers (OQ360720 and OQ360719 for B. ovis), and (OP991838 for T. annulata).

Conclusions: Taken together, this study confirms a high prevalence of piroplasmosis and offers epidemiological and molecular insights into blood parasites in sheep from Upper Egypt, highlighting the importance of detecting these parasites in various hosts and their competent vectors (ticks).

lood parasites pose a significant threat to livestock production in southern Egypt, yet there is a scarcity of information regarding their circulation and epidemiology in sheep in this region. This study aimed to investigate the seroprevalence of blood parasite infections in sheep in Assiut governorate, Upper Egypt.

Methods: A total of 400 blood samples were collected from sheep of varying ages and genders. The preliminary screening for the presence of piroplasms, mainly Babesia and Theileria spp., via microscopic examination, followed by investigation of the potential risk factors linked with the exposure to infection. Moreover, molecular identification of both parasites on some of positive samples was performed using PCR targeting Babesia 18S rRNA and Theileria annulata Tams1 gene.

Results: The microscopic examination revealed that among the examined sheep, there was an overall prevalence of blood parasites at 44% (176 out of 400), with Babesia spp. observed in 14% (56 out of 400) and Theileria spp. in 30% (120 out of 400). Furthermore, the infection rate was non-significantly higher in young animals (50%) compared to adults (38.5%) (P = 0.246). Male sheep exhibited a significantly higher vulnerability to both parasites' infection (63.3%) compared to females (35.7%) (P = 0.011). Interestingly, the prevalence of both blood parasites was significantly higher during the cold season (66.1%) compared to the hot season (15.9%) (P = <0.001). The molecular analysis identified the presence of Babesia ovis and Theileria annulata among a subsample of the positive sheep's bloods films. The identified species were recorded in the GenBank™ databases and assigned specific accession numbers (OQ360720 and OQ360719 for B. ovis), and (OP991838 for T. annulata).

Conclusions: Taken together, this study confirms a high prevalence of piroplasmosis and offers epidemiological and molecular insights into blood parasites in sheep from Upper Egypt, highlighting the importance of detecting these parasites in various hosts and their competent vectors (ticks).

Urinary bladder cancer (BC) is the ninth most common cancer worldwide. At present, the clinical diagnosis of BC depends on self-reported symptoms, tissue biopsy specimens by cystoscopy and from voided urine cytology. However, cystoscopy is an invasive examination and voided urine cytology has low sensitivity, which might provoke misdiagnosis. The search for cancer biomarkers in blood is worthy of intense attention due to patients' comfort and ease of sampling. This work aimed to study expression of mRNA metadherin (MTDH) in plasma, serum BC specific antigen 1 (BLCA-1) and cystatin C as biomarkers of BC and their relation to different disease stages. This study included 59 BC patients, 11 patients with benign bladder lesion and 18 subjects as normal controls. MTDH expression was assessed by real time polymerase chain reaction, BLCA-1, and cystatin C by the enzyme linked immunosorbent assay. The three biomarkers were elevated in BC patients than patients with benign bladder diseases and controls. Patients with BC grade 3 and 4 had higher cystatin C, BLCA-1 and MTDH in comparison to patients with grade 1 and grade 2 (p= 0.000). The receiver operating characteristic curve analysis showed that BLCA-1 at a cutoff point of 32.5 ng/ml and area under the curve of 1.00, had 100% accuracy, 100% sensitivity, 100% specificity, 100% positive predictive values and 100% negative predictive value. In conclusion, BLCA-1 was a better biomarker than cystatin C and MTDH. Cystatin C, BLCA-1 and MTDH levels, can differentiate between benign bladder lesion and BC and correlated with tumor grades. especially with OL-HDF …

Abstract
Co-infection between Intestinal parasites and Helicobacter pylori (H. pylori) in humans is a frequent health problem,
particularly in developing countries. In addition to having similar risk factors, both pathogen groups are significant contributors to
gastrointestinal disease in those with these co-infections and can influence the host's immune response. Intestinal parasitic infections
are considered as a prevalent disease globally, affecting approximately 3.5 billion individuals, particularly in developing countries.
These infections can result in anaemia, growth retardation in children and other mental and physical health problems. H. pylori is
the most prevalent chronic bacterial infection in humans globally affecting about 4.4 billion individuals worldwide. H. pylori
infection is associated with chronic gastritis, peptic ulcers and development of gastric malignant tumors. Due to their similar modes
of infection and environmental conditions, intestinal parasites and H. pylori frequently co-infect each other. The objectives of this
review were to study the association between different types of intestinal parasites and H. pylori infection. Data was collected
scrutinizing PubMed using a mesh search. Initial word searches included Helicobacter pylori; Intestinal protozoa; Entameba
histolytica; Giardia lamblia; intestinal helminthes.

B S T R A C T
Ticks, particularly Rhipicephalus annulatus, pose significant threats to livestock, causing economic losses and
transmitting various infectious diseases. This study aimed to evaluate the potential acaricidal properties of garlic
oil and its nanoemulsion against ticks infesting cattle, Rhipicephalus annulatus through the evaluation of mortality
rate and morphological changes of the treated ticks. The study also included prevalence, risk factors, and molecular
confirmation of tick species. Genetic characterization confirmed the identity of R. annulatus. Our results
revealed a high prevalence of R. annulatus (46.9%) with a higher risk in male cattle (50%) than females (44.9%)
and a nonsignificant high infection (49.1%) in animals ≤ 1 year old. The acaricidal efficiency of garlic oil and its
nanoemulsion was concentration and time-dependent. The high concentration of garlic oil (20 mg/L) induced
complete mortality within 48 hours. The nanoemulsion formulation enhanced efficacy, particularly at 5 mg/L,
which exhibited rapid and substantial acaricidal activity. Scanning electron microscopy revealed morphological
alterations induced by garlic oil and its nanoemulsion, including changes to the anterior capitulum, dorsal, and
ventral cuticles. The study contributes to the exploration of effective, safe, and eco-friendly alternatives for tick
control. Further research is warranted to validate their efficacy under diverse conditions and assess practical
strategies.