Abstract The present study was conducted for one year period extended from April 2018 to March 2019 which aimed to evaluate some epidemiological, clinical and diagnostic state of tropical theileriasis and tick infestation on cattle in New-Valley Governorate. For this purpose a total of 414 cattle of different ages, sexes and from different localities were examined for Theileria annulata infection and tick infestation. The result of tick infestation on cattle showed that the prevalence was 116 out of 414 examined samples (28%) were positive for tick infestation. Only one tick species, Hyalomma anatolicum was identified during the present study. Also, the results revealed that 160/414 (38.65%) of examined cattle were positive for Theileria annulata infection