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Characterization of aroma profiles of chinese four most famous traditional red-cooked chickens using GC–MS, GC-IMS, and E-nose

Research Abstract

The aroma profile of the four most popular types of red-cooked chickens in China was analyzed using a combination of gas chromatography-mass spectrometry (GC–MS), gas chromatography-ion mobility spectrometry (GC-IMS), and electronic nose (E-nose). Principal component analysis (PCA) demonstrated that the E-nose could successfully distinguish between the four types of red-cooked chickens. Additionally, a fingerprint was created using GC-IMS to examine the variations in volatile organic compounds (VOCs) distribution in the four chicken types. A total number of 84 and 62 VOCs were identified in the four types of red-cooked chickens using GC–MS and GC-IMS, respectively. Odor activity value (OAV) showed that 1-octen-3-ol, heptanal, hexanal, nonanal, octanal, eugenol, dimethyl trisulfide, anethole, anisaldehyde, estragole, and eucalyptol were the key volatile components in all samples. Furthermore, partial least squares-discriminant analysis (PLS-DA) demonstrated that (E, E)-2,4-decadienal, dimethyl trisulfide, octanal, eugenol, hexanal, (E)-2-nonenal, 1-octen-3-ol, butanal, ethyl acetate, ethyl acetate (D), nonanal, and heptanal could be used as markers to distinguish aroma of the four types of red-cooked chickens. Also, it is worth noting that levels of VOCs varied between chicken breast muscle and skin. The obtained results offer theoretical and technological support for flavor identification and control in red-cooked chickens to enhance their quality and encourage consumer consumption, which will be advantageous for the red-cooked chicken production chain. 

Research Authors
Xiangxiang Sun, Yumei Yu, Ahmed SM Saleh, Xinyu Yang, Jiale Ma, Ziwu Gao, Dequan Zhang, Wenhao Li, Zhenyu Wang
Research Date
Research Journal
Food Research International
Research Member
Research Publisher
Elsevier
Research Vol
173
Research Website
https://www.sciencedirect.com/science/article/abs/pii/S0963996923008803
Research Year
2023

Exploring the interaction between myofibrillar proteins and pyrazine compounds: Based on molecular docking, molecular dynamics simulation, and multi-spectroscopy techniques

Research Abstract

Flavor is one of the most important factors that affect consumers' preference for processed meat products. This study aimed to investigate effects of heating on interaction between myofibrillar proteins (MPs) and pyrazine compounds and understand the underlying mechanisms. A combination of multispectral, molecular docking, and molecular dynamics technologies was used to achieve study's aim. Results demonstrated that MPs underwent structural reconstruction and expansion during heating, which significantly altered surface hydrophobicity and SH content. MPs' zeta potential reduced from −7.29 to −10.47 when a short heating time. Additionally, a positive correlation was found between β-sheet content and ability of MPs to adsorb pyrazine compounds. Molecular docking analysis revealed 13 binding sites for pyrazines and MPs. Furthermore, amino acid residues and pyrazine compounds were found to interact by four different forms of forces, primarily van der Waals forces, carbon‑hydrogen bonds, alkyl groups, and π-alkyl groups. Obtained results demonstrated that adequate or optimized heat treatment could expose more binding sites, hence enhancing the binding of MPs to pyrazine compounds. This study may be used to better understand how structural changes in MPs during processing affect MPs' capacity to bind flavor substances, which can help improve flavor of processed meats to encourage their consumption.

Research Authors
Yumei Yu, Ahmed SM Saleh, Xiangxiang Sun, Zhenyu Wang, Yang Lu, Dequan Zhang, Chunjiang Zhang
Research Date
Research Journal
International Journal of Biological Macromolecules
Research Member
Research Publisher
Elsevier
Research Vol
253
Research Website
https://www.sciencedirect.com/science/article/abs/pii/S0141813023037418
Research Year
2023

Plant phenolic extracts for the quality protection of frying oil during deep frying: Sources, effects, and mechanisms

Research Abstract

Protection of frying oil from deterioration by adding plant phenolic extracts to guarantee the quality of fried foods becomes the primary approach to promote the sustainable development of deep frying. Therefore, sources, antioxidant effects, and mechanisms of plant phenolic extracts recently applied in the quality protection of frying oil as well as challenges for the actual use of these extracts are comprehensively reviewed in this study. Spices, herbs, berries, tea leaves, and fruit and vegetable wastes are common sources for preparing phenolic extracts showing comparative antioxidant capacity referring to the synthetic antioxidants. The general effect of using these natural antioxidants is the improvement of thermal stability to extend the shelf life of frying oil and thus the modification of edible quality of fried foods. Specifically, the increases in common quality attributes and amount of hazardous products and the oxidative reduction of unsaturated triacylglycerols without negatively influencing the sensory quality are inhibited when suitable plant extracts are applied. The incorporation of plant phenolic extracts other than synthetic counterparts in frying oil has been demonstrated as a potential method to improve the frying performance of oils. However, challenges for the scale application of plant phenolic extracts, such as the purity, thermal stability, and antioxidant timing, are still needed to be further investigated.

Research Authors
Fa Wang, Yixi Sun, Shanshan Li, Jing Yan, Wen Qin, Ahmed SM Saleh, Qing Zhang
Research Date
Research Journal
Grain & Oil Science and Technology
Research Member
Research Publisher
Elsevier
Research Website
https://www.sciencedirect.com/science/article/pii/S2590259823000195
Research Year
2023

Structural changes induced by ultrasound improve the ability of the myofibrillar protein to bind flavor compounds from spices

Research Abstract

Effects of ultrasound (UT) treatments on the structural, physicochemical, and functional properties of myofibrillar proteins (MPs), as well as their ability to bind to flavor compounds from spices, were investigated. The results demonstrated that UT treatment enhanced surface hydrophobicity, SH content, and absolute ζ-potential value of the MPs. Atomic force microscopy analysis displayed formation of MPs aggregates with small particle size in the UT-treated MPs samples. Meanwhile, UT treatment could improve the emulsifying properties and physical stability of MPs’ emulsion. Additionally, the MPs gel network structure and stability significantly improved following UT treatment. Changes in the structural, physicochemical, and functional properties enhanced the ability of MPs to bind to flavor substances from spices depending on the duration of UT treatment. Furthermore, correlation analysis showed that the ability of myristicin, anethole, and estragole to bind to MPs was highly correlated with surface hydrophobicity, ζ-potential value, and α-helix content of MPs. The results of this study may help in understanding the relationship between the changes in MPs properties during the processing of meat products and their ability to bind to flavors from spices, thereby improving flavors retention and taste of processed meat products. 

Research Authors
Xiangxiang Sun, Yumei Yu, Ahmed SM Saleh, Xinyu Yang, Jiale Ma, Ziwu Gao, Wenhao Li, Zhenyu Wang, Dequan Zhang
Research Date
Research Journal
Ultrasonics Sonochemistry
Research Member
Research Pages
106510
Research Publisher
Elsevier
Research Vol
98
Research Website
https://www.sciencedirect.com/science/article/pii/S1350417723002225
Research Year
2023

Reduction of the microbiological action during sugar beet extraction with focus on nitrite contamination

Research Abstract

Nitrite is one of the harmful pollutants for both people and animals. The formation of nitrite during the manufacturing of beet sugar was investigated. The results indicated that the cossettes contained lower nitrite content (1.23±0.1 mg/kg DM). However, the nitrite mainly formed during the extraction and purification processes. Most of the nitrite was transferred to molasses, with the remaining portion returning to the crystallization process through the circulation of juices. The nitrite content of raw juice was lower than 10 mg/kg DM in the aerobic extraction system, and it was reduced by 50% during the liming and carbonation. In the tower extraction system for both factories F1 and F2, the nitrite content increased from 5.5 and 25 mg/kg DM in the raw juice to 298 and 247%, 284 and 238%, 716 and 1032%, and 307 and 881% in the thin juice, thick juice, raw sugar green, and molasses, respectively. A significant reduction in the nitrite content of raw and de-foamed juice was achieved by the suggested point (L3) for dosing the disinfectant B (hop β-acids). The effect of the disinfectant lasted for more than 6 h at normal nitrite levels of juice and up to 90 min at high nitrite levels. Furthermore, the nitrite contents were reduced in thin juice, thick juice, and molasses by 58, 48 and 30%, respectively. By optimizing the dosing procedure and point of disinfectant hop β-acids dosage, the microbial load on the extraction system and formation rate of nitrite could be reduced significantly.

Research Authors
El-Sayed Abdel-Rahman, Thomas Frankenfeld, Ahmed SM Saleh, Eckhard Flöter
Research Date
Research Journal
Assiut Journal of Agricultural Sciences
Research Member
Research Pages
108-126
Research Publisher
Assiut University
Research Vol
54(2)
Research Website
https://ajas.journals.ekb.eg/article_294137.html
Research Year
2023

Toxicological Impact of Certain Pesticides on Honeybee, Apis melliferea L. (Hymenoptera: Apidae) under Iaboratory Conditions

Research Abstract

Honeybees exposed to pesticides direct or indirect ways through their food
research trips. Herein, this study examined the toxicity effects on laboratory of
seven tested pesticides insecticides from different pesticide groups. emamectin
benzoate, imidacloprid, chlorpyrifos, indoxacarb, lambada cyhalothrine,
glyphosate and thiophanate-methyl on honeybee workers, Apis mellifera L.
through different exposure periods of time at 24, 48, and 72 hours. The selected
pesticides were ranked based on their toxicity from the most to least one as
following: emamectin benzoate, imidacloprid, chlorpyrifos, indoxacarb, and
lambda cyhalothrine. Further, thiophanate-methyl and glyphosate were the lowest
toxicity among selected pesticides. The highest toxic pesticide during the exposure
period 24, 48 and 72 hrs was emamectin benzoate with LC50 values (0.247, 0.047
and 0.020 ppm) and with LC90 values (5.752, 0.302 and 0.072 ppm) respectively,
and the least one was glyphosate with LC50 values of (6861.151, 3366.968 and
2477.267 ppm) and LC90 values of (28243.795, 9033.695 and 6203.485 ppm) after
24, 48 and 72hr of exposure respectively. However, chlorpyrifos ranked the third
toxic pesticide at 24 and 48 hrs of treatment with LC50 values of 10.226 and 2.731
ppm and with LC90 values of (101.224 and 7.496 ppm for 24 and 48 hrs,
respectively). Whereas, after 72 hrs chlorpyrifos recorded the fourth toxic pesticide
with LC502.086 ppm and LC90 5.179ppm. Based on the toxicity index, results
showed that, the most to least toxic pesticides were arranged as followed:
emamectin benzoate>imidacloprid >chlorpyrifos >indoxacarb >lambada
cyhalothrine> thiophanate -methyl > glyphosate. These results demonstrated that
pesticides are very toxic to honeybee workers and must avoid applying them
during the times when bees are most active such as during flowering periods.
 

Research Authors
Mohammed A.A. Saad1; Aly A. Abd-Ella1*; Gamal A.M. Abdu-Allah1; Hosam ElDin A. Ezz El-Din1; Hend A. Mahmoud2 and Ahmed M.M. Ahmed
Research Department
Research Journal
Assiut Journal of Agricultural Sciences
Research Year
2023

Appearance of Pomegranate Butterfly Deudorix livia Klug (Lepidoptera: Lycaenidae) on Growth Stages of Different Host Plants and Its Fluctuation on Pomegranate Manfaloty Cultivar in Assiut Governorate, Northern Upper Egypt

Research Abstract

Incidence of mature and immature stages of pomegranate butterfly, Deudorix livia Klug (Lepidoptera: Lycaenidae) was studied regarding to some host plants phenology during growth stages. The hosts are sweet acacia, Acacia farnesiana L., and pomegranate, Punica granatum L.. Besides, study its eggs population fluctuation on Manfaloty pomegranate cultivar during 2020 and 2021 under climatic factors [Maximum (MaxT) and Minimum (MinT) Temp. in Celsius degree (°C), Relative humidity (RH %), and Wind Speed (km/hr.)]. The sweet acacia was the most favourable for D. livia and represented the main host plant during the absence of targeted stage of other host plants. Eggs and adults were first observed after 2 and 7 weeks from fruit setting on sweet acacia; respectively in 2020 and 2021. On pomegranate the previous stages appeared after 3 weeks of fruit formation in both seasons. October was the best month for egg deposition in both seasons, because egg averages highly increased and this was the damaging time for pomegranate fruits. The populations of eggs showed three peaks in 2020 and two in 2021. The correlation coefficient between average numbers of eggs and maximum wind speed was negative and highly significant (r= -0.36776, P= <.0001) in 2020, meanwhile in 2021 still negative with non-significant effect (r= -0.10447, P= 0.1410). In conclusion, determine pest peaks is important to establish control program before peaks occurrence or after infestation reaches to economic threshold levels. This will be important to directed control strategies to prevent pest from laying eggs on pomegranate fruits.

Research Authors
Farouk A. Abdel- Galil, Sara E. Mousa, Gaber H. Abo El-haggag, Mohammed Allam, and Ahmed M.M. Ahmed
Research Date
Research Department
Research Image
Research Journal
Assiut Journal of Agricultural Sciences
Research Member
Research Pages
174-188
Research Vol
54
Research Website
https://ajas.journals.ekb.eg/article_286876.html
Research Year
2023

Effects of Acetylsalicylic Acid, Echinacea Purpurea Extract, and Vitamin C On Survival, Immunity and Performance of Honey Bees

Research Abstract

From late autumn until the end of winter conditions, bees are exposed to many stress factors including low air temperature and several pathogens. This study aimed to estimate the effects of three commercial materials (acetylsalicylic acid, Echinacea purpurea extract, and vitamin (C) mixed with sugar feeding on some parameters related to stress tolerance using laboratory and field tests. The laboratory tests supported the potential role of vitamin C and Echinacea purpurea extract to enhance the tolerance ability of bees to low temperatures. The most promising results for the recovery time of bees after exposure to low temperatures, survival rates after narcosis, and hemocyte count were recorded with these two materials. After winter the strength of bee colonies fed on sugar feeding mixed with vitamin C and Echinacea purpurea extract were approximately equal to the strength before winter. However, the contrary was obtained with acetylsalicylic acid and the control group. Thus, using vitamin C or Echinacea purpurea extract as safe additives can boost the survival of bee colonies during the winter period.

Research Authors
Hossam Abou-Shaara, Abdulraouf Amro, Eslam Omar
Research Date
Research Department
Research Journal
Egyptian Academic Journal of Biological Sciences. A, Entomology
Research Member
Research Pages
81-91
Research Publisher
Egyptian Academic Journal of Biological Sciences
Research Vol
16
Research Year
2023

Bee pollen: Clinical trials and patent applications

Research Abstract

Bee pollen is a natural cocktail of floral nectar, flower pollen, enzymes, and salivary secretions produced by honeybees. Bee pollen is one of the bee products most enriched in proteins, polysaccharides, polyphenols, lipids, minerals, and vitamins. It has a significant health and medicinal impact and provides protection against many diseases, including diabetes, cancer, infectious, and cardiovascular. Bee pollen is commonly promoted as a cost-effective functional food. In particular, bee pollen has been applied in clinical trials for allergies and prostate illnesses, with a few investigations on cancer and skin problems. However, it is involved in several patents and health recipes to combat chronic health problems. This review aimed to highlight the clinical trials and patents involving bee pollen for different cases and to present the role of bee pollen as a supplementary food and a potential product in cosmetic applications.

Research Authors
Jari S Algethami, Aida A Abd El-Wahed, Mohamed H Elashal, Hanan R Ahmed, Esraa H Elshafiey, Eslam M Omar, Yahya Al Naggar, Ahmed F Algethami, Qiyang Shou, Sultan M Alsharif, Baojun Xu, Awad A Shehata, Zhiming Guo, Shaden AM Khalifa, Kai Wang, Hesham R El-
Research Date
Research Department
Research Journal
Nutrients
Research Member
Research Publisher
MDPI
Research Vol
14
Research Year
2022

Crushing corn pollen grains increased diet digestibility and hemolymph protein content while decreasing honey bee consumption

Research Abstract

A honey bee colony’s ability to grow and develop is dependent on adequate nutrition. Bees collect pollen from flowers as a source of protein, fat, vitamins, and minerals. The crude protein content of corn pollen is considered low, around 15%; however, bees frequently visit the male flowers of the tassels for pollen. In this study, we aimed for the first time to improve the nutritious value of corn pollen by mechanically crushing its external pollen wall. We then compared the effect of feeding crushed vs. non-crushed corn pollen grains on honey bee diet consumption, digestibility, hemolymph protein content, hypopharyngeal gland (HPG) size, and thorax weight under laboratory conditions. We found that crushing corn pollen grains increased diet digestibility and hemolymph protein content while decreasing honey bee pollen consumption (− 39.88%). Crushing pollen however had no effect on HPG size or thorax weight. These findings may be beneficial to beekeepers in areas where corn monoculture is prevalent. The effect of crushed corn pollen on larval development and growth, as well as colony development and vitality, should be investigated in future studies.

Research Authors
Eslam M Omar, Hesham YA Darwish, Ali A Othman, Hesham R El-Seedi, Yahya Al Naggar
Research Date
Research Department
Research Journal
Apidologie
Research Member
Research Pages
52
Research Publisher
Springer Paris
Research Vol
53
Research Year
2022
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