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Understanding interactions among flavor compounds from spices and myofibrillar proteins by multi-spectroscopy and molecular docking simulation

Research Abstract

Influence of the constant heating treatment on structural and adsorption properties of myofibrillar proteins (MPs) of chicken was investigated. The results showed that heat treatment enhanced the exposure of sulfhydryl groups and improved hydrophobicity of MPs surface. Particle size distribution of MPs significantly varied depending on heat treatment duration. Also, heat treatments resulted in significant changes in the α-helix and β-sheet structures of MPs. Besides, the MPs formed larger, irregular, and cluster-like aggregates after heat treatments. Moreover, heat treatments increased viscosity and surface roughness of MPs, while zeta potential value was reduced after heat treatments. Furhthermore, binding interactions between the MPs and spices flavors signifcanlty varied relying on nature of MPs and flavor compounds, as well as heat treatments duration. Amino acid residues were interacted with flavor compounds of spices via a variety of bonds and a stable MPs-flavors complex was performed. The obtained results provide a basis for understanding structural and physicochemical changes that occur in MPs during cooking and the interactions between MPs and flavors of spices.

Research Authors
Xiangxiang Sun, Yumei Yu, Ahmed SM Saleh, Xinyu Yang, Jiale Ma, Wenhao Li, Dequan Zhang, Zhenyu Wang
Research Date
Research Journal
International Journal of Biological Macromolecules
Research Member
Research Pages
188-198
Research Publisher
Elsevier
Research Vol
229
Research Website
https://www.sciencedirect.com/science/article/pii/S0141813022032330
Research Year
2023

Effects of various microwave intensities collaborated with different cold plasma duration time on structural, physicochemical, and digestive properties of lotus root starch

Research Abstract

Lotus root starch was treated with single and combined microwave (300 and 700 W) and cold plasma (60, 90, and 120 s) treatments. Effects of treatments on multi-structural, physicochemical, and digestive properties of lotus root starch were investigated. The results revealed that a combination of cold plasma and microwave treatments significantly affected the morphology of starch granules and reduced the relative crystallinity of starch compared with a single treatment. However, no changes were found in the chemical functional groups of starch after single or combined treatments. Additionally, the amylose content, amylopectin branch chain length distribution, solubility, and swelling power of the starch significantly varied depending on cold plasma treatment duration and microwave power. Furthermore, the treated starch showed lower peak viscosity and higher pasting temperature than the native one. Moreover, the resistant starch content significantly decreased as cold plasma treatment was prolonged and microwave power increased.

Research Authors
Xiangxiang Sun, Zhuangzhuang Sun, Ahmed SM Saleh, Yifan Lu, Xiuyun Zhang, Xiangzhen Ge, Huishan Shen, Xiuzhu Yu, Wenhao Li
Research Date
Research Journal
Food Chemistry
Research Member
Research Pages
30 March 2023, 134837
Research Publisher
Elsevier
Research Vol
405
Research Website
https://www.sciencedirect.com/science/article/pii/S0308814622027996
Research Year
2023

Effect of extrusion parameters on the interaction between rice starch and glutelin in the preparation of reconstituted rice

Research Authors
Li-shuang Wang, Yu-min Duan, Li-feng Tong, Xiao-shuai Yu, Ahmed SM Saleh, Zhi-gang Xiao, Peng Wang
Research Date
Research Journal
International Journal of Biological Macromolecules
Research Member
Research Pages
277-285
Research Publisher
Elsevier
Research Vol
225
Research Website
https://www.sciencedirect.com/science/article/pii/S0141813022025521
Research Year
2023

Fabrication of biodegradable blend plastic from konjac glucomannan/zein/PVA and understanding its multi-scale structure and physicochemical properties

Research Abstract

Exploration and synthesis of degradable plastics can alleviate and avoid environmental pollution induced by petroleum-based plastics. In this study, a konjac glucomannan (KGM)/zein/PVA ternary blend plastic was successfully prepared by casting. The results showed that, despite the presence of particle aggregation from incompatible components in blend plastic, the addition of KGM and zein improved its compatibility which is consistent with the formation of continuous dark regions and the reduction of roughness average (Ra) results in the AFM characterization. Also, XRD and FT-IR results indicated that the addition of KGM and zein disrupted the molecular and crystalline structure of PVA, induced stretching vibration of alcohol and hydroxyl groups, and crystallinity reduction. In addition, KGM deacetylation (d-KGM) reduced the intramolecular hydroxyl groups, reduced the water absorption and water vapor transmission rate of the blend plastics, and increased the crystallization temperature (Tc) and melting temperature (Tm). Furthermore, the blended plastics exhibited the best tensile strength (TS), elongation at break (E), and elastic modulus (EM) when the proportion of KGM to zein was 9:1. Notably, the blended plastic with KGM and zein added displayed more pores and cracks after soil burial, implying that the lack of degradability of pure PVA plastic was improved.

Research Authors
Wei Liang, Jiayu Zheng, Ahmed SM Saleh, Wenqing Zhao, Xinyue Liu, Chunyan Su, Mengting Yan, Xiangzhen Ge, Huishan Shen, Gulnazym Ospankulova, Kakimova Zhainagul Kh, Wenhao Li
Research Date
Research Journal
International Journal of Biological Macromolecules
Research Member
Research Pages
172-184
Research Publisher
Elsevier
Research Vol
225
Research Website
https://www.sciencedirect.com/science/article/pii/S0141813022024412
Research Year
2022

Influence of Pomegranate Butterfly, Virachola livia (Klug)(Lepidoptera: Lycaenidae) Infestation on Chemical Composition of Pomegranate Fruits

Research Abstract

The pomegranate butterfly, Virachola livia (Klug), is one of the most important pests in Egypt affects the quantity and quality of fruits. At the end of the season, 20 healthy fruits and 20 infested fruits were collected from Manfaloty, Higazy and Araby cultivars to determine the effect of infestation on some chemical compositions of pomegranate fruits (juice and peels). Results showed that the infestation of pomegranate butterfly reduced the percentage of total soluble solids (14.73, 13.93 and 13.90%), TSS/acidity ratio (12.42, 11.71 and 8.49%), total sugars (12.92, 12.48 and 12.34%) and total reducing sugars (11.32, 11.03 and 10.33%) and caused a significant increase of total titratable acidity (1.19,1.39 and 1.54) in the infested fruits compared to the healthy fruits TSS (16.43, 16.33 and 14.37%), TSS/acidity ratio (14.41, 14.24 and 10.97%), TS (14.88, 14.62 and 13.42%) and TRS (14.13, 13.23 and 11.19%) and TTA (1.13, 1.26 and 1.35) of Mnafaloty, Higazy and Araby cultivars, respectively. In addition, the infestation of this pest also affects the chemical composition of pomegranate peels which reduced the percentage of moisture (72.96, 65.53 and 61.78%), protein (4.52, 4.07 and 3.62%), Potassium (12.70, 10.72 and 10.01 g/kg) and caused a significant increase in total carbohydrates (28.89, 27.27 and 12.93%), Calcium (3.26, 3.70 and 3.95 g/kg) and pH values (3.87, 3.90 and 4.19) in the infested fruits compared to the healthy fruits moisture (74.53, 67.08 and 62.76%), protein (4.93, 4.28 and 4.91%), potassium (15.92, 13.16 and 12.21g/kg), total carbohydrates (22.37, 25.51 and 12.21%), Calcium (2.59, 2.54 and 3.21g/kg) and pH values (4.09, 3.99 and 4.46). These results show that the pomegranate butterfly infestation affects the chemical composition of fruits and decreased the economic importance of pomegranate. Therefore, an integrated control program must be developed for this pest to improve the quality and quantity of pomegranate fruits and to reduce its damage to the crop.

Keywords: Pomegranate, Virachola livia, chemical composition, cultivars

Research Authors
Aly Abd-Ella, Hazem Thabet Abu Omira, Gaber Hassan Abou-Elhagag, Mahmoud Abd El-Hamid Mahmoud, Ibrahim Abdel-Wahab Mohamed
Research Date
Research Department
Research Journal
Assiut Journal of Agricultural Sciences
Research Pages
39-51
Research Publisher
Assiut University, Faculty of Agriculture
Research Vol
53 (2)
Research Website
https://ajas.journals.ekb.eg/
Research Year
2022

Weed Control, Growth, and Yield of Tomato After Application of Metribuzin and Different Pendimethalin Products in Upper Egypt.

Research Abstract

Weeds are very problematic for tomato production worldwide. Differences in formulations of the same herbicide have different effects on weeds and crops. There are no published studies on the effect of the capsule suspension of pendimethalin (Pend) products on tomato in Egypt. The present study aims at evaluating three pre-plant Pend products compared with a post-plant metribuzin (Met) and hand hoeing on their efficiency on weed control and on the growth, yield, yield attributes, stand loss rate of tomato plants, and their economic benefit implications in tomato production. During the fall-winter seasons of 2019/2020 and 2020/2021, six weed control treatments were studied including three pre-plant Pend products, a post-plant Met, hand hoeing, and un-weeded control in tomato field experiments. Weed density, fresh weight [FW], and FW reduction% were measured. Tomato measurements included stand loss rate, vegetative growth parameters, leaf chlorophyll concentration, fruit diameter and length, marketable and total yields, fruit total soluble solids, and pH. All herbicides and hand hoeing treatments significantly reduced weeds FW. Stand loss rates of tomato were 0% in hand hoeing followed by Sencor (~ 9.3–11.1%). Vegetative growth and leaf chlorophyll concentration were improved in all treated plots as compared to the control. The highest significant increases in tomato branch number, plant height, stem diameter, and shoot FW were observed in Sencor and Mostmicro treatments. The highest marketable and total fruit yields were observed with Sencor. Met had the highest benefit–cost ratio in the study. All herbicides were effective against various noxious weeds, but tomato “hybrid 65,010” was more tolerant to Met which resulted in better yields than those obtained with Pend products. The most cost-effective method of weed control was Met.

Keywords:Solanum lycopersicum; Herbicides; Hand hoeing; Plant stand loss; Vegetative growth; Marketable and total yield

Research Authors
Ibrahim A. Mohamed & Reham M. Abdalla
Research Date
Research Department
Research Journal
Journal of Soil Science and Plant Nutrition
Research Pages
XX
Research Publisher
the Springer Nature
Research Rank
Q2
Research Vol
XX
Research Website
https://doi.org/10.1007/s42729-022-01093-3.
Research Year
2023

Sublethal effects of herbicides clethodim, haloxyfop-P-methyl, and their mixture on honey bee health.

Research Abstract

 Herbicides are the most frequently used pesticides, accounting for more than 60% of all pesticides used in agriculture around the world. Clethodim (CTD) (Vixtol  Super® 24% EC), haloxyfop-p-methyl (HFM) (Gallant  Super®10.8% EC), and their mixture (CTD (15%) + HFM (7.5%) (Fine®22.5% EC) are among the most  commonly used post-emergence graminicides in Egypt and worldwide. However, little data on their toxicological effects on honey bees (Apis melliferaL.) is known. In this study, the lethal and sublethal effects of CTD or HFM and their mixture were assessed in the laboratory on foragers and nurse honey bee workers, respectively. In the acute toxicity test, HFM was more toxic to forager workers   (LC50= 0.95 mg a.i./ml) after 96 h of oral exposure, followed by CTD then the mixture which was the least toxic one. In a chronic toxicity test, newly emerged bees were fed sugar syrup that was herbicide-free (control) or sugar syrup that contained a sublethal concentration of HFM (0.2 mg/ml; 1/5 of 96 h-LC50) or CTD (0.6 mg/ml; 1/5 96 h-LC50) or the mixture (0.2 mg/ml; 1/15 96 h-LC50) for 20 days. CTD significantly reduced bee survival and dry body weight compared to nonexposed control bees and bees exposed to HFM or the mixture. Chronic sublethal exposure to these herbicides also caused a considerable reduction in hypopharyngeal glan (HPG) acini volume, severe morphologic alterations, and a deleterious impact on hemocyte account and differentiation. This is the first study of its kind to indicate that herbicides that inhibit acetyl-CoA-carboxylase (ACCase) have a negative impact on honey bee 
health at sublethal levels.
Keywords: honey bees / herbicides / hypopharyngeal glands / hemolymph / sublethal concentrations

 

Research Authors
Ibrahim A. Mohamed , Eslam M. omar , Amer I. Tawfik , Abdulraouf M. A. amro , and Yahya al Naggar
Research Date
Research Department
Research Journal
Apidologie
Research Pages
2
Research Publisher
© INRAE, DIB and Springer-Verlag France SAS, part of Springer Nature, 2022
Research Rank
Q1
Research Vol
54
Research Website
https://doi.org/10.1007/s13592-022-00982-x
Research Year
2023

Neuro-hepatopathological changes in juvenile Oreochromis niloticus exposed to sublethal concentrations of commercial herbicides

Research Abstract

The current study estimates the impact of different common herbicides on antioxidant defenses and histological 
structure of liver and spinal cord of juvenile tilapia. Eighty-four fish were divided into seven groups: group 1 fish 
acted as controls and the remaining fish were exposed to sublethal concentrations of acetochlor, bispyribac- 
sodium, bentazon, bensulfuron-methyl, halosulfuron-methyl, or quinclorac at sublethal concentrations 2.625, 
0.800, 36.00, 2.50, 1.275, and 11.250 mg/l, respectively, for 96 h. Antioxidant parameters changed in response 
to some test herbicides and the greatest effects were caused by exposure to acetochlor and quinelorac for all 
antioxidant measurements. Prominent histological changes in liver tissue included loss of liver architecture and 
the appearance of fatty liver cells, necrotic areas, foci of leukocytic infiltration and many apoptotic cells. The 
most obvious changes in the spinal cord in all treated fish were degradation of myelinated white matter fibers 
with the emergence of empty spaces, large aggregation of pyknotic neuroglial nuclei, and damaged areas in the 
dorsal horn of gray matter. Collectively, the harmful effect of tested herbicides on antioxidant capacity and 
significant alterations in histological structures of liver and spinal cord of Oreochromis niloticus.

Keywords: Nile tilapia; Antioxidant activity; Liver; Spinal cord; Histological structure

Research Authors
Shaimaa M.M. Saleh, Ibrahim A. Mohamed, Mohamed Fathy, Alaa El-Din H. Sayed
Research Date
Research Department
Research Journal
Environmental Toxicology and Pharmacology
Research Pages
103871
Research Publisher
Elsevier B.V.
Research Rank
Q1
Research Vol
93
Research Website
https://doi.org/10.1016/j.etap.2022.103871
Research Year
2022

Multi-biomarkers approach to assess the toxicity of novel insecticide (Voliam flexi®) on Clarias gariepinus: From behavior to immunotoxicity.

Research Abstract

This study was conducted to determine for the first time the immunological, histopathological, histochemical, 
and ultrastructural changes; hematological and biochemical alterations; and poikilocytosis induced in Clarias 
gariepinus by Voliam flexi® 40% WG (thiamethoxam +chlorantraniliprole). Beside control fish, juvenile 
C. gariepinus were subjected to three sublethal concentrations of Voliam flexi® (43.5, 87.5, and 175 mg/L) for 15
days. Voliam flexi® induced immunotoxic impairments in C. gariepinus, such as a decrease in some immunity 
variables (lysozyme and phagocyte activity, immunoglobulin concentration, and nitro blue tetrazolium level). It 
also caused an extreme increase in the levels of primary cytokines (interleukin-1β and IL-6), compared with the 
control. The toxic effects of Voliam flexi® increased gradually with the increasing concentrations tested. Histological examination of the liver demonstrated necrosis, vacuolated hepatocytes (fatty deposition), melanomacrophage centers, foci of inflammatory cells, congested and dilated blood sinusoids, hepatic degeneration, fibrosis increment (Sirius Red stain), and glycogen depletion, as well as cytopathological alterations. We conclude that the toxic effects of Voliam flexi® must be restricted or prevented by using control mechanisms in aquatic systems

Keywords: 
Thiamethoxam; Chlorantraniliprole; Clarias gariepinus; IL-1β; Immunity

Research Authors
Ibrahim A. Mohamed , Mohamed Hamed , Hanem S. Abdel-Tawab , Salwa Mansour , Hamdy A.M. Soliman, Jae-Seong Lee , Alaa El-Din H. Sayed
Research Date
Research Department
Research Journal
Fish & shellfish immunology
Research Pages
54–64
Research Publisher
Elsevier Ltd.
Research Rank
Q1
Research Vol
125
Research Website
https://doi.org/10.1016/j.fsi.2022.04.051
Research Year
2022

Hepato-morpholoy and biochemical studies on the liver of albino rats after exposure to glyphosate-Roundup®.

Research Abstract

Background:The object of this work was to evaluate of the hepatic effects of the herbicides glyphosate-Roundup®
by different doses in both sexes of albino rats.
Methods:Forty animals divided into four groups with ten animals for each (both sexes) were treated orally with
vehicle (controls) and 25, 50, and 100 mg/kg bwt of glyphosate-Roundup® (treated groups) for 15 days daily.
Results:The most conspicuous changes occurred on the liver treated groups due to glyphosate toxicity were the
increase of enzymes activities of ALT and AST, cellular infiltration, many signs of nucleus degeneration, focal
necrosis, rarified cytoplasm, disorganization of cellular organelles, and deposition of lipid droplets. The increase in
the amount of collagenous fibers and the number of the mast cell were also observed.
Conclusion:Our results indicated that the administration of glyphosate-Roundup® in different doses may cause
adverse effects on the histopathological, ultrastructure, and biochemical alternations on the liver of the albino rats.
Keywords:Glyphosate-Roundup®, ALT and AST, Liver, Histopathology, Ultrastructure

Research Authors
Shaimaa M. M. Saleh , Tasneem A. Elghareeb , Mohamed A. I. Ahmed , Ibrahim A. Mohamed and Hosam A. Ezz El-Din
Research Date
Research Department
Research Journal
The Journal of Basic and Applied Zoology
Research Pages
48
Research Publisher
the Springer Nature
Research Vol
79
Research Website
https://doi.org/10.1186/s41936-018-0060-4
Research Year
2018
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