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ABSTRACT : This is the first report of attempt to identify QTL(s) for the Beet Cyst Nematode (Heterodera schachtii Schm) resistant segregating in an F2 population of the oil radish (Raphanus sativus L.) cross ‘Pegletta x Silettanova’. A total of 290 F2 individuals were derived from self-pollinating single F1 plant of the cross ‘Pegletta x Silettanova’. The F2 individuals distributed in two discreet genotype classes, complying with the assumption of monogenic dominant inheritance of the BCN resistance in oil radish. Bulked segregant analysis (BSA) revealed 16 RAPD and AFLP markers differentiating the resistance and the susceptible bulks based on band intensity. The RAPD OPD-04-568 and the AFLP E39M48-268(D) markers were the most differentiated candidates be the resistant and the susceptible bulks. Quantitative trait analysis was applied using MAPQTL 4.0 computer software. The Kruskal-Wallis and IM results the identified one major QTL dispread on one arm of linkage group 6. Multiple QTL Model (MQM) analyses indicated that the BCN resistance is controlled by one major QTL on LG 6. Moreover, the RAPD marker OPD-04-568 and the AFLP marker E39M48-268(D) were candidate to flanking the QTL of BCN resistance. These markers explained 62% and 48% of the total phenotypic variability, respectively. The detected QTL explanted 83% of the total phenotypic variations. The implications of these findings for breeders are discussed.

ABSTRACT : This is the first report of attempt to identify QTL(s) for the Beet Cyst Nematode (Heterodera schachtii Schm) resistant segregating in an F2 population of the oil radish (Raphanus sativus L.) cross ‘Pegletta x Silettanova’. A total of 290 F2 individuals were derived from self-pollinating single F1 plant of the cross ‘Pegletta x Silettanova’. The F2 individuals distributed in two discreet genotype classes, complying with the assumption of monogenic dominant inheritance of the BCN resistance in oil radish. Bulked segregant analysis (BSA) revealed 16 RAPD and AFLP markers differentiating the resistance and the susceptible bulks based on band intensity. The RAPD OPD-04-568 and the AFLP E39M48-268(D) markers were the most differentiated candidates be the resistant and the susceptible bulks. Quantitative trait analysis was applied using MAPQTL 4.0 computer software. The Kruskal-Wallis and IM results the identified one major QTL dispread on one arm of linkage group 6. Multiple QTL Model (MQM) analyses indicated that the BCN resistance is controlled by one major QTL on LG 6. Moreover, the RAPD marker OPD-04-568 and the AFLP marker E39M48-268(D) were candidate to flanking the QTL of BCN resistance. These markers explained 62% and 48% of the total phenotypic variability, respectively. The detected QTL explanted 83% of the total phenotypic variations. The implications of these findings for breeders are discussed.

ABSTRACT : This is the first report of attempt to identify QTL(s) for the Beet Cyst Nematode (Heterodera schachtii Schm) resistant segregating in an F2 population of the oil radish (Raphanus sativus L.) cross ‘Pegletta x Silettanova’. A total of 290 F2 individuals were derived from self-pollinating single F1 plant of the cross ‘Pegletta x Silettanova’. The F2 individuals distributed in two discreet genotype classes, complying with the assumption of monogenic dominant inheritance of the BCN resistance in oil radish. Bulked segregant analysis (BSA) revealed 16 RAPD and AFLP markers differentiating the resistance and the susceptible bulks based on band intensity. The RAPD OPD-04-568 and the AFLP E39M48-268(D) markers were the most differentiated candidates be the resistant and the susceptible bulks. Quantitative trait analysis was applied using MAPQTL 4.0 computer software. The Kruskal-Wallis and IM results the identified one major QTL dispread on one arm of linkage group 6. Multiple QTL Model (MQM) analyses indicated that the BCN resistance is controlled by one major QTL on LG 6. Moreover, the RAPD marker OPD-04-568 and the AFLP marker E39M48-268(D) were candidate to flanking the QTL of BCN resistance. These markers explained 62% and 48% of the total phenotypic variability, respectively. The detected QTL explanted 83% of the total phenotypic variations. The implications of these findings for breeders are discussed.

ABSTRACT : This is the first report of attempt to identify QTL(s) for the Beet Cyst Nematode (Heterodera schachtii Schm) resistant segregating in an F2 population of the oil radish (Raphanus sativus L.) cross ‘Pegletta x Silettanova’. A total of 290 F2 individuals were derived from self-pollinating single F1 plant of the cross ‘Pegletta x Silettanova’. The F2 individuals distributed in two discreet genotype classes, complying with the assumption of monogenic dominant inheritance of the BCN resistance in oil radish. Bulked segregant analysis (BSA) revealed 16 RAPD and AFLP markers differentiating the resistance and the susceptible bulks based on band intensity. The RAPD OPD-04-568 and the AFLP E39M48-268(D) markers were the most differentiated candidates be the resistant and the susceptible bulks. Quantitative trait analysis was applied using MAPQTL 4.0 computer software. The Kruskal-Wallis and IM results the identified one major QTL dispread on one arm of linkage group 6. Multiple QTL Model (MQM) analyses indicated that the BCN resistance is controlled by one major QTL on LG 6. Moreover, the RAPD marker OPD-04-568 and the AFLP marker E39M48-268(D) were candidate to flanking the QTL of BCN resistance. These markers explained 62% and 48% of the total phenotypic variability, respectively. The detected QTL explanted 83% of the total phenotypic variations. The implications of these findings for breeders are discussed.

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