Background We aim to report the short-term and long-term outcome of surgical repair following Roux-en-Y hepaticojejunostomy for postcholecystectomy bile duct injury (BDI) in a tertiary hepatobiliary center in Upper Egypt (Assiut University). Patients and methods A retrospective study was conducted on all patients diagnosed with postcholecystectomy BDI during the period 2014–2018 at Al-Rajhi Liver Institute, Assiut University, who had undergone surgical repair of BDI. Patients were grouped according to the time interval between previous surgery and surgical repair into two groups: group 1: time interval less than or equal to 6 weeks and group 2: time interval more than 6 weeks. Results Of 43 patients enrolled, 18 were in group 1 and 25 in group 2. The overall incidence of stricture was 14% (27.9% in group 1 and 4% in group 2), which is statistically significant (P=0.026). Other factors that have a significant association with incidence of stricture included bilirubin level. On multivariate analysis, none of these factors had a significant effect on the development of biliary stricture. Conclusion We believe that timing of repair of BDI following cholecystectomy has a significant effect on the outcome of repair. Moreover, best results in biliary reconstruction can be achieved in a specialized hepatobiliary center.

Background We aim to report the short-term and long-term outcome of surgical repair following Roux-en-Y hepaticojejunostomy for postcholecystectomy bile duct injury (BDI) in a tertiary hepatobiliary center in Upper Egypt (Assiut University). Patients and methods A retrospective study was conducted on all patients diagnosed with postcholecystectomy BDI during the period 2014–2018 at Al-Rajhi Liver Institute, Assiut University, who had undergone surgical repair of BDI. Patients were grouped according to the time interval between previous surgery and surgical repair into two groups: group 1: time interval less than or equal to 6 weeks and group 2: time interval more than 6 weeks. Results Of 43 patients enrolled, 18 were in group 1 and 25 in group 2. The overall incidence of stricture was 14% (27.9% in group 1 and 4% in group 2), which is statistically significant (P=0.026). Other factors that have a significant association with incidence of stricture included bilirubin level. On multivariate analysis, none of these factors had a significant effect on the development of biliary stricture. Conclusion We believe that timing of repair of BDI following cholecystectomy has a significant effect on the outcome of repair. Moreover, best results in biliary reconstruction can be achieved in a specialized hepatobiliary center.

Background We aim to report the short-term and long-term outcome of surgical repair following Roux-en-Y hepaticojejunostomy for postcholecystectomy bile duct injury (BDI) in a tertiary hepatobiliary center in Upper Egypt (Assiut University). Patients and methods A retrospective study was conducted on all patients diagnosed with postcholecystectomy BDI during the period 2014–2018 at Al-Rajhi Liver Institute, Assiut University, who had undergone surgical repair of BDI. Patients were grouped according to the time interval between previous surgery and surgical repair into two groups: group 1: time interval less than or equal to 6 weeks and group 2: time interval more than 6 weeks. Results Of 43 patients enrolled, 18 were in group 1 and 25 in group 2. The overall incidence of stricture was 14% (27.9% in group 1 and 4% in group 2), which is statistically significant (P=0.026). Other factors that have a significant association with incidence of stricture included bilirubin level. On multivariate analysis, none of these factors had a significant effect on the development of biliary stricture. Conclusion We believe that timing of repair of BDI following cholecystectomy has a significant effect on the outcome of repair. Moreover, best results in biliary reconstruction can be achieved in a specialized hepatobiliary center.

Background We aim to report the short-term and long-term outcome of surgical repair following Roux-en-Y hepaticojejunostomy for postcholecystectomy bile duct injury (BDI) in a tertiary hepatobiliary center in Upper Egypt (Assiut University). Patients and methods A retrospective study was conducted on all patients diagnosed with postcholecystectomy BDI during the period 2014–2018 at Al-Rajhi Liver Institute, Assiut University, who had undergone surgical repair of BDI. Patients were grouped according to the time interval between previous surgery and surgical repair into two groups: group 1: time interval less than or equal to 6 weeks and group 2: time interval more than 6 weeks. Results Of 43 patients enrolled, 18 were in group 1 and 25 in group 2. The overall incidence of stricture was 14% (27.9% in group 1 and 4% in group 2), which is statistically significant (P=0.026). Other factors that have a significant association with incidence of stricture included bilirubin level. On multivariate analysis, none of these factors had a significant effect on the development of biliary stricture. Conclusion We believe that timing of repair of BDI following cholecystectomy has a significant effect on the outcome of repair. Moreover, best results in biliary reconstruction can be achieved in a specialized hepatobiliary center.

Background: Our study aimed to evaluate the clinical utility of detecting plasma microRNAs (miR-21 & miR-92a) for diagnosis of colorectal cancer patients and its relation to tumor staging. Patients and Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 and miR-92a in serum. The sensitivity and specificity of these markers were evaluated by receiver operating characteristic (ROC) curve analysis. Final staging of colorectal cancer cases was assigned according to results of histopathologic examination of surgically resected specimens. Results: This study included 52 cases of colorectal cancer (CRC), 20 cases of precancerous colorectal lesions, and 20 healthy controls. Both Plasma miR-21 and Plasma miR-92a were significantly higher in CRC group compared to both the control group and precancerous group. Also, they were significantly higher in advanced CRC stages than early CRC stages. The sensitivity and specificity of miR-21 for discriminating CRC from controls were found to be 90.38% and 100.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 94.23% and 100.0%, respectively. For discriminating CRC cases from precancerous lesions, the sensitivity and specificity of miR-21 were found to be 75.08% and 95.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 80.77% and 100.0%, respectively. Conclusions: both plasma miR-21 and miR-92a have significant value for early detection of CRC as non-invasive screening molecular biomarkers with high sensitivity and specificity. They also help for differentiation between patients with benign and malignant colorectal lesions and those with early and advanced CRC.

Background: Our study aimed to evaluate the clinical utility of detecting plasma microRNAs (miR-21 & miR-92a) for diagnosis of colorectal cancer patients and its relation to tumor staging. Patients and Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 and miR-92a in serum. The sensitivity and specificity of these markers were evaluated by receiver operating characteristic (ROC) curve analysis. Final staging of colorectal cancer cases was assigned according to results of histopathologic examination of surgically resected specimens. Results: This study included 52 cases of colorectal cancer (CRC), 20 cases of precancerous colorectal lesions, and 20 healthy controls. Both Plasma miR-21 and Plasma miR-92a were significantly higher in CRC group compared to both the control group and precancerous group. Also, they were significantly higher in advanced CRC stages than early CRC stages. The sensitivity and specificity of miR-21 for discriminating CRC from controls were found to be 90.38% and 100.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 94.23% and 100.0%, respectively. For discriminating CRC cases from precancerous lesions, the sensitivity and specificity of miR-21 were found to be 75.08% and 95.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 80.77% and 100.0%, respectively. Conclusions: both plasma miR-21 and miR-92a have significant value for early detection of CRC as non-invasive screening molecular biomarkers with high sensitivity and specificity. They also help for differentiation between patients with benign and malignant colorectal lesions and those with early and advanced CRC.

Background: Our study aimed to evaluate the clinical utility of detecting plasma microRNAs (miR-21 & miR-92a) for diagnosis of colorectal cancer patients and its relation to tumor staging. Patients and Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 and miR-92a in serum. The sensitivity and specificity of these markers were evaluated by receiver operating characteristic (ROC) curve analysis. Final staging of colorectal cancer cases was assigned according to results of histopathologic examination of surgically resected specimens. Results: This study included 52 cases of colorectal cancer (CRC), 20 cases of precancerous colorectal lesions, and 20 healthy controls. Both Plasma miR-21 and Plasma miR-92a were significantly higher in CRC group compared to both the control group and precancerous group. Also, they were significantly higher in advanced CRC stages than early CRC stages. The sensitivity and specificity of miR-21 for discriminating CRC from controls were found to be 90.38% and 100.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 94.23% and 100.0%, respectively. For discriminating CRC cases from precancerous lesions, the sensitivity and specificity of miR-21 were found to be 75.08% and 95.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 80.77% and 100.0%, respectively. Conclusions: both plasma miR-21 and miR-92a have significant value for early detection of CRC as non-invasive screening molecular biomarkers with high sensitivity and specificity. They also help for differentiation between patients with benign and malignant colorectal lesions and those with early and advanced CRC.

Background: Our study aimed to evaluate the clinical utility of detecting plasma microRNAs (miR-21 & miR-92a) for diagnosis of colorectal cancer patients and its relation to tumor staging. Patients and Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 and miR-92a in serum. The sensitivity and specificity of these markers were evaluated by receiver operating characteristic (ROC) curve analysis. Final staging of colorectal cancer cases was assigned according to results of histopathologic examination of surgically resected specimens. Results: This study included 52 cases of colorectal cancer (CRC), 20 cases of precancerous colorectal lesions, and 20 healthy controls. Both Plasma miR-21 and Plasma miR-92a were significantly higher in CRC group compared to both the control group and precancerous group. Also, they were significantly higher in advanced CRC stages than early CRC stages. The sensitivity and specificity of miR-21 for discriminating CRC from controls were found to be 90.38% and 100.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 94.23% and 100.0%, respectively. For discriminating CRC cases from precancerous lesions, the sensitivity and specificity of miR-21 were found to be 75.08% and 95.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 80.77% and 100.0%, respectively. Conclusions: both plasma miR-21 and miR-92a have significant value for early detection of CRC as non-invasive screening molecular biomarkers with high sensitivity and specificity. They also help for differentiation between patients with benign and malignant colorectal lesions and those with early and advanced CRC.

Background: Our study aimed to evaluate the clinical utility of detecting plasma microRNAs (miR-21 & miR-92a) for diagnosis of colorectal cancer patients and its relation to tumor staging. Patients and Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 and miR-92a in serum. The sensitivity and specificity of these markers were evaluated by receiver operating characteristic (ROC) curve analysis. Final staging of colorectal cancer cases was assigned according to results of histopathologic examination of surgically resected specimens. Results: This study included 52 cases of colorectal cancer (CRC), 20 cases of precancerous colorectal lesions, and 20 healthy controls. Both Plasma miR-21 and Plasma miR-92a were significantly higher in CRC group compared to both the control group and precancerous group. Also, they were significantly higher in advanced CRC stages than early CRC stages. The sensitivity and specificity of miR-21 for discriminating CRC from controls were found to be 90.38% and 100.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 94.23% and 100.0%, respectively. For discriminating CRC cases from precancerous lesions, the sensitivity and specificity of miR-21 were found to be 75.08% and 95.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 80.77% and 100.0%, respectively. Conclusions: both plasma miR-21 and miR-92a have significant value for early detection of CRC as non-invasive screening molecular biomarkers with high sensitivity and specificity. They also help for differentiation between patients with benign and malignant colorectal lesions and those with early and advanced CRC.

Background: Our study aimed to evaluate the clinical utility of detecting plasma microRNAs (miR-21 & miR-92a) for diagnosis of colorectal cancer patients and its relation to tumor staging. Patients and Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 and miR-92a in serum. The sensitivity and specificity of these markers were evaluated by receiver operating characteristic (ROC) curve analysis. Final staging of colorectal cancer cases was assigned according to results of histopathologic examination of surgically resected specimens. Results: This study included 52 cases of colorectal cancer (CRC), 20 cases of precancerous colorectal lesions, and 20 healthy controls. Both Plasma miR-21 and Plasma miR-92a were significantly higher in CRC group compared to both the control group and precancerous group. Also, they were significantly higher in advanced CRC stages than early CRC stages. The sensitivity and specificity of miR-21 for discriminating CRC from controls were found to be 90.38% and 100.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 94.23% and 100.0%, respectively. For discriminating CRC cases from precancerous lesions, the sensitivity and specificity of miR-21 were found to be 75.08% and 95.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 80.77% and 100.0%, respectively. Conclusions: both plasma miR-21 and miR-92a have significant value for early detection of CRC as non-invasive screening molecular biomarkers with high sensitivity and specificity. They also help for differentiation between patients with benign and malignant colorectal lesions and those with early and advanced CRC.