Background and aim

Growing evidence highlighted the primary role of the immune system in the disease course of triple-negative breast cancer (TNBC). The study aim was to investigate the expression of PD-1 and CD39 on CD4⁺ and CD8⁺ cells infiltrating tumor tissue compared to their counterparts in peripheral blood and explore its association with tumor characteristics, disease progression, and prognosis in females with TNBC.

Patients and methods

The study included 30 TNBC patients and 20 healthy controls. Cancer and normal breast tissue and peripheral blood samples were collected for evaluation of the expression of PD-1 and CD39 on CD4⁺ and CD8⁺T cells by flow cytometry.

Results

A marked reduction in the percentage of CD8⁺ T lymphocytes and a significant increase in the frequencies of CD4⁺ T lymphocytes and CD4⁺ and CD8⁺ T lymphocytes expressing PD1 and CD39 were evident in tumor tissue in comparison with the normal breast tissue. The DFS was inversely related to the cancer tissue PD1⁺CD8⁺ and CD39⁺CD8⁺ T lymphocytes. Almost all studied cells were significantly increased in the tumor tissue than in peripheral blood. Positive correlations were detected among peripheral PD1⁺CD4⁺T lymphocytes and each of cancer tissue PD1⁺CD4⁺, PD1⁺CD8⁺and CD39⁺CD8⁺T cells, and among peripheral and cancer tissue CD39⁺CD4⁺and CD39⁺CD8⁺ T cells.

Conclusions

The CD39 and PD1 inhibitory pathways are synergistically utilized by TNBC cells to evade host immune response causing poor survival. Hence, combinational immunotherapy blocking these pathways might be a promising treatment strategy in …

CD45 is a transmembrane glycoprotein and protein tyrosine phosphatase expressed on the surface of all nucleated hematopoietic cells. While there is increasing evidence demonstrating the involvement of CD45 in immune system regulation, no information on CD45 expression in inflammation and sepsis is currently available. Therefore, we determined the CD45 surface expression on granulocytes, lymphocytes, and monocytes in patients with COVID-19 and healthy volunteers in both absence and presence of lipopolysaccharide (LPS). Following approval by the local ethics committee, whole blood samples were obtained from patients with COVID-19 infection on day 1 of hospital admission and healthy volunteers. Samples were incubated in absence and presence of LPS and CD45 was measured in granulocytes, lymphocytes, and monocytes using flow cytometry. In comparison with healthy individuals, COVID-19 patients showed an increased CD45 expression on the surface of granulocytes (+35%, p < 0.02) and lymphocytes (+39%, p < 0.0001), but a reduced CD45 expression on monocytes (−35%, p < 0.0001). LPS incubation of whole blood from healthy individuals increased the CD45 expression on granulocytes (+430%, p < 0.0001), lymphocytes (+32%, p = 0.0012), and monocytes (+36%, p = 0.0005), respectively. LPS incubation of whole blood samples from COVID-19 patients increased the CD45 expression on granulocytes and monocytes, and decreased the CD45 expression on lymphocytes. In conclusion, CD45 expression on leucocytes is altered: (1) in COVID-19 patients, and (2) in in vitro endotoxemia in a complex cell-specific

Early diagnosis and proper management of hepatocellular carcinoma (HCC) improve patient prognosis. Several studies attempted to discover new genes to understand the pathogenesis and identify the prognostic and predictive factors in HCC patients, to improve patient’s overall survival (OS) and maintain their physical and social activity. The transcription factor FOS-like antigen 1 (FOSL1) acts as one of the important prognostic factors in different tumors, and its overexpression correlates with tumors’ progression and worse patient survival. However, its expression and molecular mechanisms underlying its dysregulation in human HCC remain poorly understood. Our study was conducted to evaluate the expression of FOSL1 in HCC tissues and its relationship with various clinicopathological parameters besides OS.

Early diagnosis and proper management of hepatocellular carcinoma (HCC) improve patient prognosis. Several studies attempted to discover new genes to understand the pathogenesis and identify the prognostic and predictive factors in HCC patients, to improve patient’s overall survival (OS) and maintain their physical and social activity. The transcription factor FOS-like antigen 1 (FOSL1) acts as one of the important prognostic factors in different tumors, and its overexpression correlates with tumors’ progression and worse patient survival. However, its expression and molecular mechanisms underlying its dysregulation in human HCC remain poorly understood. Our study was conducted to evaluate the expression of FOSL1 in HCC tissues and its relationship with various clinicopathological parameters besides OS.

Background: Metastatic breast cancer (Stage IV) accounts for 3% of all newly diagnosed patients with breast cancer in Japan, which is not different from that of USA (6%) reported according to the Surveillance, Epidemiology and End Results data. The treatment of Stage IV breast cancer has traditionally been palliative care with chemotherapy, hormonal therapy and/or radiation therapy. Young patients with limited metastatic (oligometastatic) disease and an excellent performance status tend to be long-term survivors. Combined treatment for such patients including surgery, provide better chance for longterm progression-free survival than systemic therapy alone. Objective: Evaluating overall survival and progression free survival for metastatic breast cancer patients underwent mastectomy plus systemic therapy versus that on systemic therapy alone.

Patients and methods: This randomized study was conducted in surgical oncology department, medical oncology department, South Egypt Cancer Institute, Assiut University. We prospectively review and compare women presented with metastatic breast cancer between from January 2014 and December 2020, who received primary tumor resection (group A) and women treated non-operatively (group B).

Results: This study was conducted in surgical oncology department, medical oncology department-South Egypt Cancer Institute, Assiut University. we retrospectively and prospectively review and compare women (200 patients) presented with metastatic breast cancer between January 2014 and December 2019, who received surgery to the breast and women treated non-operatively. The study includes …