The current study investigated telocytes (TCs) in the intestinal bulb of Grass carp using light microscopy (LM), Transmission electron microscopy (TEM), scanning electron microscopy, and immunohistochemistry (IHC). By LM, TCs were distinguished by the typical morphological features that had a cell body and telopodes using HE, toluidine blue, methylene blue, Marsland silver stain, Grimelius’s silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon’s and Mallory triple trichrome, Van Gieson stains, Verhoef’s stain, Sudan black, osmic acid, performic acid with methylene blue and bromophenol blue. TCs were identifed under the epithelium as an individual cell or formed a TCs sheath. They detected in the lamina propria, between muscle fbers, around the myenteric plexus and fbrous tissue. TCs acquired immunological features of endocrine cells that exhibited high afnity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical staining using chromogranin A. Sub epithelial TCs were closely related to the endocrine cells. TCs and their secretory activities were recognized using acridine orange. TCs were identifed by IHC using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fbers, fbroblast, Schwann cells and nerve fbers. In conclusion, the localization of TCs in relation to diferent types of immune cells indicated their potential role in the maintenance of intestinal immunity.