The aim of this study was to investigate the influence of long-term high-protein flushing on ovarian function and metabolic status in Ossimi ewes. Fourteen cycling Ossimi ewes were divided into high protein (210 % of maintenance crude protein) or treatment group (TG; n = 7) and control group (CG; n = 7). Oestrous was synchronized by PGF2α, monitored ultrasonographically daily for two oestrous cycles. The CG fed a maintenance diet (9.5 % CP) through-out the experiment. The mean number of small (2–2.9 mm), medium (3–5 mm) ovarian follicles, and follicular waves were higher (P0.01) in ewes of TG compared to that of CG in the first and second oestrous. The size of large, subordinate and ovulatory follicles were greater (p 0.05) in ewes of TG compared to those of CG. Ewes of the TG recorded a higher (p 0.05) ovulation rate in the first cycle while, ovulation rate did not show any significant change in second oestrous. Long term flushing of high-protein ration significantly influenced the concentration of glucose and some metabolic profiles. Concentration of serum total proteins was greater at 0, 1 and 13 days, while serum total cholesterol was greater at day 0 and 1 after first oestrous (ovulation is day 0) in ewes of TG as compared to ewes of CG. Serum glucose was increased significantly (p0.05) at day 3 and 13 after first oestrous and at day 0 and 7 after second ovulation in TG when comparing to CG. There was significant (p 0.05) increase in serum concentrations of progesterone at day 13 after first oestrous in TG and at day 3 after second oestrous. The level of serum estradiol 17-β increased significantly at day 0, 1, 3, 7 and 13 in the first oestrous and at day 0 on second oestrous in TG as compared to CG. In conclusion, the long term protein flushing increased the number of small and medium follicles and follicular waves, the size of ovulatory follicles in the first and second oestrous, the reproductive efficiency in subtropical ewes. Long term protein flushing increase ovulation rate in the first but had no effect on the second oestrous.