A total of 100 raw cow's milk samples were randomly collected from dairy shops and street vendors (50 samples each) in Assiut city, Egypt. Samples were examined for detection of adulteration and heat treatment. Automatic milk analyzer was used for determination of density, added water %, freezing point, fat%, S.N.F% for the detection of adulteration by addition of water and partial skimming. Also, milk samples were examined by general and specific tests for detection of inhibitory substances, as well as detection of some commercial additives and heat treatment. The results revealed adulteration of milk samples in different percentages. Street vendors’ milk samples were subjected to more adulteration than that of dairy shops. It could be concluded that milk marketed in Assiut city is produced and handled under inadequate control measures allowing greedy retailers and producers to adulterate milk to increase their gain.