tIn this study were aimed to identify the association of SNPs candidate genes of TNF- and IL-6 with hor-mones levels and sperm cells death in infertile subjects of Uttar Pradesh population in North India. Thestudy population comprised, fertile donor (control group) and infertile group patients i.e. normozoosper-mic (idiopathic unexplained), oligozoospermic and asthenozoospermic groups, with 260 subjects in eachgroup. Subjects were selected from the Departments of Urology, K.G’s Medical University and Urology,SGPGIMS, Lucknow, India. The allele-specific polymerase chain reaction (PCR) and PCR-RFLP were usedto investigate the substitution of the guanine (G)-to-adenosine (A) at position-308 and guanine (G)-to-cytosine (C) at position-174 in the promoter regions of the TNF- and IL-6 genes, respectively. Furthertheir relation to male fertility and sperm function were also investigated. It was found that the substi-tution levels from G to A and from G to C in the TNF- and IL-6 genes, respectively, were significantlyhigher in the infertile subjects as compared to that of control group. The apoptosis and necrosis lev-els were also higher in oligozoospermic and asthenozoospermic infertile subjects. Further it was foundto be associated with increased level of reactive oxygen species as observed in oligozoospermic andasthenozoospermic subjects. However, a significant decrease in testosterone and luteinizing hormonewith increased prolactin and follicle stimulating hormones was observed in infertile subjects. The studypopulations indicating a strong association between TNF- G-308A and IL-6 G-174C substitution withinfertile men which is further supported by allele and genotype meta-analysis and thus established it asa risk factor.