Field experiment was performed during the 2004-2005 and 2005-2006 seasons at the Floriculture Nursery, Experimental Farm, Faculty of Agriculture, Assiut University to investigate the effects of plant spacing (15 and 30cm) and various fertilizer treatments; cattle manure (15m3/feddan), NPK fertilization [ammonium nitrate (33.5% N) 60, calcium superphosphate (15.5%P2O5) 45, and potassium sulphate(48%K2O) 48kg/feddan] and bio-fertilizers [Biogen (500g) and phosphorein (300g/Kg seeds) were added either individually or in combination]. A complete randomized block design in a split-plot arrangement with four replicates was used in this experiment. Plant spacing was randomly distributed in the main plots and fertilizer treatments in the sub plots. Data obtained showed that significant increases were found in branch number, seed production, yields of volatile and fixed oil in seeds in relation to plant spacing of 30cm comparison to that of 15 cm. Moreover, leaf contents of carbohydrates, nitrogen, phos phorous and potassium recorded similar trend. All fertilizer treatments significantly increased plant height and branch number per plant compared to unfertilized plants. However, cattle manure was more effective in this concern. Cattle manure produced higher yields of seeds and volatile oil than other treatments. In addition, it significantly increased leaf contents of carbohydrates, N,P and K. The interaction among treatments cleared that the space of 30 cm along with cattle manure yielded the best results.