The bird cherry-oat aphid, Rhopalosiphum padi (Linnaeus), is considered one of the most dangerous and destructive pests of the cereal family. It directly affects nutrition and indirectly transmits viral diseases, leading to a reduction in quantity and quality. Therefore, the use of appropriate and effective control methods is vital. In this study, the effectiveness of emamectin benzoate on the nymph stage of R. padi on wheat was evaluated using the leaf-dip method. Mortality was recorded after exposure to different concentrations of the pesticide after 24, 48, and 72 hours under laboratory conditions. The results revealed the clear effectiveness of emamectin benzoate, especially after exposure at 48 and 72 hours, with LC50 values of 0.017 and 0.004 μg/ml, respectively. Additionally, the toxicity of emamectin benzoate increased from 10.59-fold after 48 hours to 45-fold after 72 hours based on the time dependent increase in toxicity. Therefore, emamectin benzoate is an effective pesticide for controlling R. padi on wheat and a promising tool in integrated pest management programs.